Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia
West Nile virus is a mosquito-borne neurotropic pathogen with a wide host range that constitutes a significant risk to public and animal health. There is limited information on WNV infection in domesticated mammals in Malaysia; however, current reports indicate infections in birds, macaques, bats an...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/109481/1/fvets-10-1126199.pdf |
| _version_ | 1817927634597707776 |
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| author | Mohammed, Mohammed Nma Yasmin, Abd Rahaman Ramanoon, Siti Zubaidah Noraniza, Mohd Adzahan Ooi, Peck Toung Ain-Najwa, Mohd Yuseri Natasha, Jafar Ali Nur-Fazila, Saulol Hamid Arshad, Siti Suri Mohammed, Hussni Omar |
| author_facet | Mohammed, Mohammed Nma Yasmin, Abd Rahaman Ramanoon, Siti Zubaidah Noraniza, Mohd Adzahan Ooi, Peck Toung Ain-Najwa, Mohd Yuseri Natasha, Jafar Ali Nur-Fazila, Saulol Hamid Arshad, Siti Suri Mohammed, Hussni Omar |
| author_sort | Mohammed, Mohammed Nma |
| collection | UPM |
| description | West Nile virus is a mosquito-borne neurotropic pathogen with a wide host range that constitutes a significant risk to public and animal health. There is limited information on WNV infection in domesticated mammals in Malaysia; however, current reports indicate infections in birds, macaques, bats and pigs from Malaysia. In this study, 203 serum samples from cattle, goats, and horses were tested for the presence of anti-WNV IgG using a competitive enzyme-linked immunosorbent assay (c-ELISA). Additionally, using one-step RT-PCR, nasopharyngeal swabs were analyzed for WNV RNA from all 203 animals in this study. The WNV seroprevalence was 32.53% (27/83) at 95% CI (0.2342–0.4319) in cattle, 48.27% (14/29) at 95% CI (0.3139–0.6557) in goats and 53.84% (49/91) at 95% CI (0.4366–0.6373) in horses. Cross-reactive JEV antibodies were detected in two cattle and 34 horses. None of the cattle or goats tested positive for WNV RT-PCR. Seven horses were positive for WNV RT-PCR, a molecular prevalence of 7.69% (7/91) at 95% CI (0.0353–0.1528). This is the first reported detection of WNV in domesticated mammals of Malaysia, a significant addition to the growing evidence that WNV is being transmitted from vectors to susceptible hosts in Malaysia. |
| first_indexed | 2024-12-09T02:21:34Z |
| format | Article |
| id | upm.eprints-109481 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-12-09T02:21:34Z |
| publishDate | 2023 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | upm.eprints-1094812024-11-06T01:33:59Z http://psasir.upm.edu.my/id/eprint/109481/ Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia Mohammed, Mohammed Nma Yasmin, Abd Rahaman Ramanoon, Siti Zubaidah Noraniza, Mohd Adzahan Ooi, Peck Toung Ain-Najwa, Mohd Yuseri Natasha, Jafar Ali Nur-Fazila, Saulol Hamid Arshad, Siti Suri Mohammed, Hussni Omar West Nile virus is a mosquito-borne neurotropic pathogen with a wide host range that constitutes a significant risk to public and animal health. There is limited information on WNV infection in domesticated mammals in Malaysia; however, current reports indicate infections in birds, macaques, bats and pigs from Malaysia. In this study, 203 serum samples from cattle, goats, and horses were tested for the presence of anti-WNV IgG using a competitive enzyme-linked immunosorbent assay (c-ELISA). Additionally, using one-step RT-PCR, nasopharyngeal swabs were analyzed for WNV RNA from all 203 animals in this study. The WNV seroprevalence was 32.53% (27/83) at 95% CI (0.2342–0.4319) in cattle, 48.27% (14/29) at 95% CI (0.3139–0.6557) in goats and 53.84% (49/91) at 95% CI (0.4366–0.6373) in horses. Cross-reactive JEV antibodies were detected in two cattle and 34 horses. None of the cattle or goats tested positive for WNV RT-PCR. Seven horses were positive for WNV RT-PCR, a molecular prevalence of 7.69% (7/91) at 95% CI (0.0353–0.1528). This is the first reported detection of WNV in domesticated mammals of Malaysia, a significant addition to the growing evidence that WNV is being transmitted from vectors to susceptible hosts in Malaysia. Frontiers Media 2023-06-29 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/109481/1/fvets-10-1126199.pdf Mohammed, Mohammed Nma and Yasmin, Abd Rahaman and Ramanoon, Siti Zubaidah and Noraniza, Mohd Adzahan and Ooi, Peck Toung and Ain-Najwa, Mohd Yuseri and Natasha, Jafar Ali and Nur-Fazila, Saulol Hamid and Arshad, Siti Suri and Mohammed, Hussni Omar (2023) Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia. Frontiers in Veterinary Science, 10. pp. 1-8. ISSN 2297-1769 https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1126199/full 10.3389/fvets.2023.1126199 |
| spellingShingle | Mohammed, Mohammed Nma Yasmin, Abd Rahaman Ramanoon, Siti Zubaidah Noraniza, Mohd Adzahan Ooi, Peck Toung Ain-Najwa, Mohd Yuseri Natasha, Jafar Ali Nur-Fazila, Saulol Hamid Arshad, Siti Suri Mohammed, Hussni Omar Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia |
| title | Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia |
| title_full | Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia |
| title_fullStr | Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia |
| title_full_unstemmed | Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia |
| title_short | Serological and molecular surveillance of West Nile virus in domesticated mammals of Peninsular Malaysia |
| title_sort | serological and molecular surveillance of west nile virus in domesticated mammals of peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/109481/1/fvets-10-1126199.pdf |
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