The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel
Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degra...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Multidisciplinary Digital Publishing Institute
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/109545/1/plants-12-02536-v2.pdf |
| _version_ | 1824452249924927488 |
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| author | Zamree, Nur Diyanah Puasa, Nurul Aini Lim, Zheng Syuen Wong, Chiew-Yen Shaharuddin, Noor Azmi Zakaria, Nur Nadhirah Merican, Faradina Convey, Peter Ahmad, Syahida Shaari, Hasrizal Azmi, Alyza Azzura Ahmad, Siti Aqlima Zulkharnain, Azham |
| author_facet | Zamree, Nur Diyanah Puasa, Nurul Aini Lim, Zheng Syuen Wong, Chiew-Yen Shaharuddin, Noor Azmi Zakaria, Nur Nadhirah Merican, Faradina Convey, Peter Ahmad, Syahida Shaari, Hasrizal Azmi, Alyza Azzura Ahmad, Siti Aqlima Zulkharnain, Azham |
| author_sort | Zamree, Nur Diyanah |
| collection | UPM |
| description | Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degraders. Therefore, this study focused on the utilisation of an Antarctic microalga in the bioremediation of diesel. The studied microalgal strain was originally obtained from a freshwater ecosystem in Paradise Bay, western Antarctic Peninsula. When analysed in systems with and without aeration, this microalgal strain achieved a higher growth rate under aeration. To maintain the growth of this microalga optimally, a conventional one-factor-at a-time (OFAT) analysis was also conducted. Based on the optimized parameters, algal growth and diesel degradation performance was highest at pH 7.5 with 0.5 mg/L NaCl concentration and 0.5 g/L of NaNO3 as a nitrogen source. This currently unidentified microalga flourished in the presence of diesel, with maximum algal cell numbers on day 7 of incubation in the presence of 1% v/v diesel. Chlorophyll a, b and carotenoid contents of the culture were greatest on day 9 of incubation. The diesel degradation achieved was 64.5% of the original concentration after 9 days. Gas chromatography analysis showed the complete mineralisation of C7–C13 hydrocarbon chains. Fourier transform infrared spectroscopy analysis confirmed that strain WCY_AQ5_3 fully degraded the hydrocarbon with bioabsorption of the products. Morphological and molecular analyses suggested that this spherical, single-celled green microalga was a member of the genus Micractinium. The data obtained confirm that this microalga is a suitable candidate for further research into the degradation of diesel in Antarctica. |
| first_indexed | 2025-02-19T02:47:32Z |
| format | Article |
| id | upm.eprints-109545 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2025-02-19T02:47:32Z |
| publishDate | 2023 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | upm.eprints-1095452024-12-17T03:41:55Z http://psasir.upm.edu.my/id/eprint/109545/ The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel Zamree, Nur Diyanah Puasa, Nurul Aini Lim, Zheng Syuen Wong, Chiew-Yen Shaharuddin, Noor Azmi Zakaria, Nur Nadhirah Merican, Faradina Convey, Peter Ahmad, Syahida Shaari, Hasrizal Azmi, Alyza Azzura Ahmad, Siti Aqlima Zulkharnain, Azham Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degraders. Therefore, this study focused on the utilisation of an Antarctic microalga in the bioremediation of diesel. The studied microalgal strain was originally obtained from a freshwater ecosystem in Paradise Bay, western Antarctic Peninsula. When analysed in systems with and without aeration, this microalgal strain achieved a higher growth rate under aeration. To maintain the growth of this microalga optimally, a conventional one-factor-at a-time (OFAT) analysis was also conducted. Based on the optimized parameters, algal growth and diesel degradation performance was highest at pH 7.5 with 0.5 mg/L NaCl concentration and 0.5 g/L of NaNO3 as a nitrogen source. This currently unidentified microalga flourished in the presence of diesel, with maximum algal cell numbers on day 7 of incubation in the presence of 1% v/v diesel. Chlorophyll a, b and carotenoid contents of the culture were greatest on day 9 of incubation. The diesel degradation achieved was 64.5% of the original concentration after 9 days. Gas chromatography analysis showed the complete mineralisation of C7–C13 hydrocarbon chains. Fourier transform infrared spectroscopy analysis confirmed that strain WCY_AQ5_3 fully degraded the hydrocarbon with bioabsorption of the products. Morphological and molecular analyses suggested that this spherical, single-celled green microalga was a member of the genus Micractinium. The data obtained confirm that this microalga is a suitable candidate for further research into the degradation of diesel in Antarctica. Multidisciplinary Digital Publishing Institute 2023-07-03 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/109545/1/plants-12-02536-v2.pdf Zamree, Nur Diyanah and Puasa, Nurul Aini and Lim, Zheng Syuen and Wong, Chiew-Yen and Shaharuddin, Noor Azmi and Zakaria, Nur Nadhirah and Merican, Faradina and Convey, Peter and Ahmad, Syahida and Shaari, Hasrizal and Azmi, Alyza Azzura and Ahmad, Siti Aqlima and Zulkharnain, Azham (2023) The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel. Plants, 12 (13). art. no. 2536. pp. 1-16. ISSN 2223-7747 https://www.mdpi.com/2223-7747/12/13/2536 10.3390/plants12132536 |
| spellingShingle | Zamree, Nur Diyanah Puasa, Nurul Aini Lim, Zheng Syuen Wong, Chiew-Yen Shaharuddin, Noor Azmi Zakaria, Nur Nadhirah Merican, Faradina Convey, Peter Ahmad, Syahida Shaari, Hasrizal Azmi, Alyza Azzura Ahmad, Siti Aqlima Zulkharnain, Azham The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel |
| title | The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel |
| title_full | The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel |
| title_fullStr | The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel |
| title_full_unstemmed | The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel |
| title_short | The utilisation of Antarctic microalgae isolated from Paradise Bay (Antarctic Peninsula) in the bioremediation of diesel |
| title_sort | utilisation of antarctic microalgae isolated from paradise bay antarctic peninsula in the bioremediation of diesel |
| url | http://psasir.upm.edu.my/id/eprint/109545/1/plants-12-02536-v2.pdf |
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