Recombinant protein application induced defense mechanism in host plant against papaya dieback disease
Erwinia mallotivora is a Gram-negative bacterium that causes the papaya dieback disease in Malaysia. Currently, no effective disease control method is documented. In this regard, the adoption of harpin proteins in promoting plant defense mechanisms has been reported to be a promising control method...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Penerbit Universiti Teknologi Malaysia
2023
|
| _version_ | 1811137941980839936 |
|---|---|
| author | Bakar, Ros Azrinawati Hana Abu Bakar, Norliza Ahmad, Khairulmazmi Shaharuddin, Noor Azmi Abdul Wahab, Mohd As’wad Juri, Nor Mustaiqazah |
| author_facet | Bakar, Ros Azrinawati Hana Abu Bakar, Norliza Ahmad, Khairulmazmi Shaharuddin, Noor Azmi Abdul Wahab, Mohd As’wad Juri, Nor Mustaiqazah |
| author_sort | Bakar, Ros Azrinawati Hana |
| collection | UPM |
| description | Erwinia mallotivora is a Gram-negative bacterium that causes the papaya dieback disease in Malaysia. Currently, no effective disease control method is documented. In this regard, the adoption of harpin proteins in promoting plant defense mechanisms has been reported to be a promising control method for this disease. This study used, two recombinant harpin proteins, Hrp I and Hrp II, to control the disease in the glasshouse and field conditions. The results of foliar application in the glasshouse showed protective index of 70.8% and 35.7% for Hrp I and Hrp II, respectively. Meanwhile, the field trial for Hrp I showed a promising protection index of 72.3%. Moreover, the plant growth-promoting attributes were also significantly improved in the case of the Hrp I -treated plants. The effectiveness of Hrp I and II as inducers of systemic acquired resistance (SAR) was further validated by profiling defense-related genes using RT-qPCR analysis. The results showed that the treated papaya plants with Hrp I possess the highest expression of defense genes such as peroxidase, osmotin, and PRID in the glasshouse and field samples. In conclusion, the application of Hrp I is a promising disease control approach in managing papaya dieback disease in Malaysia. |
| first_indexed | 2024-09-25T03:42:18Z |
| format | Article |
| id | upm.eprints-110035 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:42:18Z |
| publishDate | 2023 |
| publisher | Penerbit Universiti Teknologi Malaysia |
| record_format | dspace |
| spelling | upm.eprints-1100352024-09-09T02:12:57Z http://psasir.upm.edu.my/id/eprint/110035/ Recombinant protein application induced defense mechanism in host plant against papaya dieback disease Bakar, Ros Azrinawati Hana Abu Bakar, Norliza Ahmad, Khairulmazmi Shaharuddin, Noor Azmi Abdul Wahab, Mohd As’wad Juri, Nor Mustaiqazah Erwinia mallotivora is a Gram-negative bacterium that causes the papaya dieback disease in Malaysia. Currently, no effective disease control method is documented. In this regard, the adoption of harpin proteins in promoting plant defense mechanisms has been reported to be a promising control method for this disease. This study used, two recombinant harpin proteins, Hrp I and Hrp II, to control the disease in the glasshouse and field conditions. The results of foliar application in the glasshouse showed protective index of 70.8% and 35.7% for Hrp I and Hrp II, respectively. Meanwhile, the field trial for Hrp I showed a promising protection index of 72.3%. Moreover, the plant growth-promoting attributes were also significantly improved in the case of the Hrp I -treated plants. The effectiveness of Hrp I and II as inducers of systemic acquired resistance (SAR) was further validated by profiling defense-related genes using RT-qPCR analysis. The results showed that the treated papaya plants with Hrp I possess the highest expression of defense genes such as peroxidase, osmotin, and PRID in the glasshouse and field samples. In conclusion, the application of Hrp I is a promising disease control approach in managing papaya dieback disease in Malaysia. Penerbit Universiti Teknologi Malaysia 2023 Article PeerReviewed Bakar, Ros Azrinawati Hana and Abu Bakar, Norliza and Ahmad, Khairulmazmi and Shaharuddin, Noor Azmi and Abdul Wahab, Mohd As’wad and Juri, Nor Mustaiqazah (2023) Recombinant protein application induced defense mechanism in host plant against papaya dieback disease. Jurnal Teknologi (Sciences and Engineering), 85 (6). pp. 187-199. ISSN 0127-9696; ESSN: 2180-3722 https://journals.utm.my/jurnalteknologi/article/view/20092 10.11113/jurnalteknologi.v85.20092 |
| spellingShingle | Bakar, Ros Azrinawati Hana Abu Bakar, Norliza Ahmad, Khairulmazmi Shaharuddin, Noor Azmi Abdul Wahab, Mohd As’wad Juri, Nor Mustaiqazah Recombinant protein application induced defense mechanism in host plant against papaya dieback disease |
| title | Recombinant protein application induced defense mechanism in host plant against papaya dieback disease |
| title_full | Recombinant protein application induced defense mechanism in host plant against papaya dieback disease |
| title_fullStr | Recombinant protein application induced defense mechanism in host plant against papaya dieback disease |
| title_full_unstemmed | Recombinant protein application induced defense mechanism in host plant against papaya dieback disease |
| title_short | Recombinant protein application induced defense mechanism in host plant against papaya dieback disease |
| title_sort | recombinant protein application induced defense mechanism in host plant against papaya dieback disease |
| work_keys_str_mv | AT bakarrosazrinawatihana recombinantproteinapplicationinduceddefensemechanisminhostplantagainstpapayadiebackdisease AT abubakarnorliza recombinantproteinapplicationinduceddefensemechanisminhostplantagainstpapayadiebackdisease AT ahmadkhairulmazmi recombinantproteinapplicationinduceddefensemechanisminhostplantagainstpapayadiebackdisease AT shaharuddinnoorazmi recombinantproteinapplicationinduceddefensemechanisminhostplantagainstpapayadiebackdisease AT abdulwahabmohdaswad recombinantproteinapplicationinduceddefensemechanisminhostplantagainstpapayadiebackdisease AT jurinormustaiqazah recombinantproteinapplicationinduceddefensemechanisminhostplantagainstpapayadiebackdisease |