Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer
Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study repr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Elsevier BV
2023
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| _version_ | 1825939813143937024 |
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| author | Jaapar, F. Nadhirah Parmin, N.A. A. Halim, N. Hamidah Hashim, Uda Gopinath, Subash C.B. Halim, F. Syakirah Uda, M.N.A. Afzan, Amilia Mohamad Nor, N. Abdul Razak, Khairunisak |
| author_facet | Jaapar, F. Nadhirah Parmin, N.A. A. Halim, N. Hamidah Hashim, Uda Gopinath, Subash C.B. Halim, F. Syakirah Uda, M.N.A. Afzan, Amilia Mohamad Nor, N. Abdul Razak, Khairunisak |
| author_sort | Jaapar, F. Nadhirah |
| collection | UPM |
| description | Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study represents surface charge transduction of micro-interdigitated electrodes (micro-IDE) alumina insulated with silica, independent and mini genosensor modified with colloidal gold NPs (AuNPs), and determination of gene hybridization for early detection of cervical cancer. The surface of AuNPs deposited micro-IDE functionalized with optimized 3-aminopropyl- triethoxysilane (APTES) followed by hybridization with deoxyribonucleic acid (DNA) virus to develop DNA genosensor. The results of ssDNA hybridization with the ssDNA target of human papillomavirus (HPV) 18 have affirmed that micro-IDE functionalized with colloidal AuNPs resulted in the lowest detection at 0.529 aM. Based on coefficient regression, micro-IDE functionalized with AuNPs produces better results in the sensitivity test (R2 = 0.99793) than unfunctionalized micro-IDE. |
| first_indexed | 2024-09-25T03:42:30Z |
| format | Article |
| id | upm.eprints-110098 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:42:30Z |
| publishDate | 2023 |
| publisher | Elsevier BV |
| record_format | dspace |
| spelling | upm.eprints-1100982024-09-05T07:00:12Z http://psasir.upm.edu.my/id/eprint/110098/ Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer Jaapar, F. Nadhirah Parmin, N.A. A. Halim, N. Hamidah Hashim, Uda Gopinath, Subash C.B. Halim, F. Syakirah Uda, M.N.A. Afzan, Amilia Mohamad Nor, N. Abdul Razak, Khairunisak Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study represents surface charge transduction of micro-interdigitated electrodes (micro-IDE) alumina insulated with silica, independent and mini genosensor modified with colloidal gold NPs (AuNPs), and determination of gene hybridization for early detection of cervical cancer. The surface of AuNPs deposited micro-IDE functionalized with optimized 3-aminopropyl- triethoxysilane (APTES) followed by hybridization with deoxyribonucleic acid (DNA) virus to develop DNA genosensor. The results of ssDNA hybridization with the ssDNA target of human papillomavirus (HPV) 18 have affirmed that micro-IDE functionalized with colloidal AuNPs resulted in the lowest detection at 0.529 aM. Based on coefficient regression, micro-IDE functionalized with AuNPs produces better results in the sensitivity test (R2 = 0.99793) than unfunctionalized micro-IDE. Elsevier BV 2023 Article PeerReviewed Jaapar, F. Nadhirah and Parmin, N.A. and A. Halim, N. Hamidah and Hashim, Uda and Gopinath, Subash C.B. and Halim, F. Syakirah and Uda, M.N.A. and Afzan, Amilia and Mohamad Nor, N. and Abdul Razak, Khairunisak (2023) Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer. International Journal of Biological Macromolecules, 253 (pt.2). art. no. 126745. pp. 1-14. ISSN 0141-8130; ESSN: 1879-0003 https://linkinghub.elsevier.com/retrieve/pii/S0141813023036425 10.1016/j.ijbiomac.2023.126745 |
| spellingShingle | Jaapar, F. Nadhirah Parmin, N.A. A. Halim, N. Hamidah Hashim, Uda Gopinath, Subash C.B. Halim, F. Syakirah Uda, M.N.A. Afzan, Amilia Mohamad Nor, N. Abdul Razak, Khairunisak Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer |
| title | Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer |
| title_full | Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer |
| title_fullStr | Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer |
| title_full_unstemmed | Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer |
| title_short | Micro-interdigitated electrodes genosensor based on au-deposited nanoparticles for early detection of cervical cancer |
| title_sort | micro interdigitated electrodes genosensor based on au deposited nanoparticles for early detection of cervical cancer |
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