Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats
Secondary osteoporosis is mainly caused by prolong used of glucocorticoid treatment. The Piper sarmentosum leaf aqueous extract was found to exhibit bone formatting osteocalcin activity against Dexamethasone induced osteoporotic rats. Thirty-two Sprague-Dawley rats were divided equally into four g...
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| Format: | Article |
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Universiti Putra Malaysia Press
2023
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| _version_ | 1811137971195215872 |
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| author | Mohd Ramli, Elvy Suhana Soelaiman, Ima Nirwana Mohd Thani, Suryati Mohd Nor, Nurul Huda Mohamad Zainal, Nurul Hayati Masrudin, Siti Saleha Mohamad Asri, Siti Fadziyah |
| author_facet | Mohd Ramli, Elvy Suhana Soelaiman, Ima Nirwana Mohd Thani, Suryati Mohd Nor, Nurul Huda Mohamad Zainal, Nurul Hayati Masrudin, Siti Saleha Mohamad Asri, Siti Fadziyah |
| author_sort | Mohd Ramli, Elvy Suhana |
| collection | UPM |
| description | Secondary osteoporosis is mainly caused by prolong used of glucocorticoid treatment. The Piper sarmentosum
leaf aqueous extract was found to exhibit bone formatting osteocalcin activity against Dexamethasone induced
osteoporotic rats. Thirty-two Sprague-Dawley rats were divided equally into four groups - G1: Sham-operated control
group given intramuscular (IM) olive oil as vehicle and normal saline orally as vehicle; G2: Adrenalectomized
(Adrx) control group given IM dexamethasone (DEX) (120 μg/kg/day) and normal saline orally as vehicle; G3: Adrx
group given IM DEX (120 μg/kg/day) and aqueous extract of Piper sarmentosum leaves (125 mg/kg/day) orally; and
G4: Adrx group given IM DEX (120 μg/kg/day) and glycyrrhizic acid (GCA) (120 mg/kg/day) orally.
Immunohistochemical method with gold labelling was used to label the osteocalcin protein. Silver brightener
was used, sprinkled on gold with a size of 5 nm so that the resulting image can be seen more clearly using
a light microscope. The osteocalcin protein was measured quantitatively based on nomenclature report of the
ASBMR Histomorphometry Committee (American Society for Bone Mineral Research). The activity shown by
immunohisto-gold expression and localization of osteocalcin was comparable with the reference, glycyrrhizic
acid, a potent inhibitor of 11β-hydroxysteroid dehydrogenase enzyme in RANKL-OPG pathway. As a conclusion,
Piper sarmentosum may one day be utilized as an alternate treatment for individuals receiving long-term
glucocorticoid therapy to prevent osteoporosis, therefore osteoporotic fractures. |
| first_indexed | 2024-09-25T03:42:46Z |
| format | Article |
| id | upm.eprints-110208 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:42:46Z |
| publishDate | 2023 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-1102082024-07-05T07:32:51Z http://psasir.upm.edu.my/id/eprint/110208/ Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats Mohd Ramli, Elvy Suhana Soelaiman, Ima Nirwana Mohd Thani, Suryati Mohd Nor, Nurul Huda Mohamad Zainal, Nurul Hayati Masrudin, Siti Saleha Mohamad Asri, Siti Fadziyah Secondary osteoporosis is mainly caused by prolong used of glucocorticoid treatment. The Piper sarmentosum leaf aqueous extract was found to exhibit bone formatting osteocalcin activity against Dexamethasone induced osteoporotic rats. Thirty-two Sprague-Dawley rats were divided equally into four groups - G1: Sham-operated control group given intramuscular (IM) olive oil as vehicle and normal saline orally as vehicle; G2: Adrenalectomized (Adrx) control group given IM dexamethasone (DEX) (120 μg/kg/day) and normal saline orally as vehicle; G3: Adrx group given IM DEX (120 μg/kg/day) and aqueous extract of Piper sarmentosum leaves (125 mg/kg/day) orally; and G4: Adrx group given IM DEX (120 μg/kg/day) and glycyrrhizic acid (GCA) (120 mg/kg/day) orally. Immunohistochemical method with gold labelling was used to label the osteocalcin protein. Silver brightener was used, sprinkled on gold with a size of 5 nm so that the resulting image can be seen more clearly using a light microscope. The osteocalcin protein was measured quantitatively based on nomenclature report of the ASBMR Histomorphometry Committee (American Society for Bone Mineral Research). The activity shown by immunohisto-gold expression and localization of osteocalcin was comparable with the reference, glycyrrhizic acid, a potent inhibitor of 11β-hydroxysteroid dehydrogenase enzyme in RANKL-OPG pathway. As a conclusion, Piper sarmentosum may one day be utilized as an alternate treatment for individuals receiving long-term glucocorticoid therapy to prevent osteoporosis, therefore osteoporotic fractures. Universiti Putra Malaysia Press 2023 Article PeerReviewed Mohd Ramli, Elvy Suhana and Soelaiman, Ima Nirwana and Mohd Thani, Suryati and Mohd Nor, Nurul Huda and Mohamad Zainal, Nurul Hayati and Masrudin, Siti Saleha and Mohamad Asri, Siti Fadziyah (2023) Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats. Malaysian Journal of Medicine and Health Sciences, 19 (suppl.12). pp. 115-119. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2023112812191414_2023-0585.pdf 10.47836/mjmhs.19.s12.14 |
| spellingShingle | Mohd Ramli, Elvy Suhana Soelaiman, Ima Nirwana Mohd Thani, Suryati Mohd Nor, Nurul Huda Mohamad Zainal, Nurul Hayati Masrudin, Siti Saleha Mohamad Asri, Siti Fadziyah Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats |
| title | Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats |
| title_full | Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats |
| title_fullStr | Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats |
| title_full_unstemmed | Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats |
| title_short | Influence of Piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid-induced osteoporotic rats |
| title_sort | influence of piper sarmentosum aqueous extract on the expression of osteocalcin in glucocorticoid induced osteoporotic rats |
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