Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats
Introduction: Benign prostatic hyperplasia (BPH) is the most prevalent prostatic disease in ageing men, characterised by an excessive proliferation of the prostatic epithelial and stromal cells. Despite the extensive choices of pharmaceutical therapies, the current treatments possess side effects, n...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Faculty of Medicine and Health Sciences, UPM
2023
|
| _version_ | 1811137977205653504 |
|---|---|
| author | Masrudin, Siti Saleha Razwan, Nur Izzah Athirah Mat Taib, Che Norma Mohd Nor, Nurul Huda Mohd Asri, Siti Fadziyah Alias, Zazali Mohamad, Jamaludin |
| author_facet | Masrudin, Siti Saleha Razwan, Nur Izzah Athirah Mat Taib, Che Norma Mohd Nor, Nurul Huda Mohd Asri, Siti Fadziyah Alias, Zazali Mohamad, Jamaludin |
| author_sort | Masrudin, Siti Saleha |
| collection | UPM |
| description | Introduction: Benign prostatic hyperplasia (BPH) is the most prevalent prostatic disease in ageing men, characterised by an excessive proliferation of the prostatic epithelial and stromal cells. Despite the extensive choices of pharmaceutical therapies, the current treatments possess side effects, necessitating the search for new alternative options, including herbal substances such as Pueraria mirifica. This tuberous root of P. mirifica is a medicinal plant that contains numerous phytoestrogens, traditionally used for health rejuvenation in aged men and women. This study was carried out to access the inhibitory effect of 5α-reductase of P. mirifica and its histoprotective effect in a rat model of testosterone-induced BPH. Methods: Adult Sprague Dawley (12 weeks) were subcutaneously injected with testosterone propionate (3 mg/kg) daily to induce BPH. Rats (n=6) in all groups (aqueous extract of P. mirifica (APM): 10, 100, and 1000 mg/kg, p.o.; finasteride: 2mg/kg, p.o., BPH model, and sham groups) were treated for 30 days. The determination of serum dihydrotestosterone (DHT) level, prostatic index and prostate structural changes were investigated. Results: APM and finasteride-treated groups showed significantly lesser prostatic weight and prostatic index, serum DHT levels compared to the model group (p<0.05). Furthermore, there was a significantly lower prostate score with improved prostate histomorphology, demonstrating fewer epithelial involutions of glandular tissues and improved stromal and epithelial cells. Conclusion: In conclusion, the aqueous extract of P. mirifica tuberous root mitigates the development of BPH and it can be inferred that aqueous extract of P. mirifica tuberous root may possess the active agents for anti-BPH treatment. |
| first_indexed | 2024-09-25T03:42:52Z |
| format | Article |
| id | upm.eprints-110239 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:42:52Z |
| publishDate | 2023 |
| publisher | Faculty of Medicine and Health Sciences, UPM |
| record_format | dspace |
| spelling | upm.eprints-1102392024-07-05T07:54:05Z http://psasir.upm.edu.my/id/eprint/110239/ Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats Masrudin, Siti Saleha Razwan, Nur Izzah Athirah Mat Taib, Che Norma Mohd Nor, Nurul Huda Mohd Asri, Siti Fadziyah Alias, Zazali Mohamad, Jamaludin Introduction: Benign prostatic hyperplasia (BPH) is the most prevalent prostatic disease in ageing men, characterised by an excessive proliferation of the prostatic epithelial and stromal cells. Despite the extensive choices of pharmaceutical therapies, the current treatments possess side effects, necessitating the search for new alternative options, including herbal substances such as Pueraria mirifica. This tuberous root of P. mirifica is a medicinal plant that contains numerous phytoestrogens, traditionally used for health rejuvenation in aged men and women. This study was carried out to access the inhibitory effect of 5α-reductase of P. mirifica and its histoprotective effect in a rat model of testosterone-induced BPH. Methods: Adult Sprague Dawley (12 weeks) were subcutaneously injected with testosterone propionate (3 mg/kg) daily to induce BPH. Rats (n=6) in all groups (aqueous extract of P. mirifica (APM): 10, 100, and 1000 mg/kg, p.o.; finasteride: 2mg/kg, p.o., BPH model, and sham groups) were treated for 30 days. The determination of serum dihydrotestosterone (DHT) level, prostatic index and prostate structural changes were investigated. Results: APM and finasteride-treated groups showed significantly lesser prostatic weight and prostatic index, serum DHT levels compared to the model group (p<0.05). Furthermore, there was a significantly lower prostate score with improved prostate histomorphology, demonstrating fewer epithelial involutions of glandular tissues and improved stromal and epithelial cells. Conclusion: In conclusion, the aqueous extract of P. mirifica tuberous root mitigates the development of BPH and it can be inferred that aqueous extract of P. mirifica tuberous root may possess the active agents for anti-BPH treatment. Faculty of Medicine and Health Sciences, UPM 2023 Article PeerReviewed Masrudin, Siti Saleha and Razwan, Nur Izzah Athirah and Mat Taib, Che Norma and Mohd Nor, Nurul Huda and Mohd Asri, Siti Fadziyah and Alias, Zazali and Mohamad, Jamaludin (2023) Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats. Malaysian Journal of Medicine and Health Sciences, 19 (suppl.9). pp. 230-239. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/jurnal_kami/volume_19_2023/mjmhs_vol19_supp_9_august_2023-75471 10.47836/mjmhs.19.s9.32 |
| spellingShingle | Masrudin, Siti Saleha Razwan, Nur Izzah Athirah Mat Taib, Che Norma Mohd Nor, Nurul Huda Mohd Asri, Siti Fadziyah Alias, Zazali Mohamad, Jamaludin Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats |
| title | Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats |
| title_full | Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats |
| title_fullStr | Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats |
| title_full_unstemmed | Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats |
| title_short | Inhibitory effects of Pueraria mirifica aqueous extracts on 5α-reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia Sprague Dawley rats |
| title_sort | inhibitory effects of pueraria mirifica aqueous extracts on 5α reductase and prostate histomorphometry in testosterone induced benign prostatic hyperplasia sprague dawley rats |
| work_keys_str_mv | AT masrudinsitisaleha inhibitoryeffectsofpuerariamirificaaqueousextractson5areductaseandprostatehistomorphometryintestosteroneinducedbenignprostatichyperplasiaspraguedawleyrats AT razwannurizzahathirah inhibitoryeffectsofpuerariamirificaaqueousextractson5areductaseandprostatehistomorphometryintestosteroneinducedbenignprostatichyperplasiaspraguedawleyrats AT mattaibchenorma inhibitoryeffectsofpuerariamirificaaqueousextractson5areductaseandprostatehistomorphometryintestosteroneinducedbenignprostatichyperplasiaspraguedawleyrats AT mohdnornurulhuda inhibitoryeffectsofpuerariamirificaaqueousextractson5areductaseandprostatehistomorphometryintestosteroneinducedbenignprostatichyperplasiaspraguedawleyrats AT mohdasrisitifadziyah inhibitoryeffectsofpuerariamirificaaqueousextractson5areductaseandprostatehistomorphometryintestosteroneinducedbenignprostatichyperplasiaspraguedawleyrats AT aliaszazali inhibitoryeffectsofpuerariamirificaaqueousextractson5areductaseandprostatehistomorphometryintestosteroneinducedbenignprostatichyperplasiaspraguedawleyrats AT mohamadjamaludin inhibitoryeffectsofpuerariamirificaaqueousextractson5areductaseandprostatehistomorphometryintestosteroneinducedbenignprostatichyperplasiaspraguedawleyrats |