Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice
Objective: To determine the involvement and the modulatory effects of IL-33 during Plasmodium berghei ANKA (PbA) infection. Methods: PbA infection in male ICR mice was utilized as a model of malaria. Systemically circulating IL-33 levels were determined in blood plasma by enzyme-linked immunosorbent...
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Wolters Kluwer Medknow Publications
2023
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| author | Abd Rachman Isnadi, Mohammad Faruq Basir, Rusliza Omenesa, Ramatu Bello Abd Majid, Roslaini Abdullah, Maizaton Atmadini Mat Taib, Che Norma Priya, Sivan Padma Kong, Yong Yean Kin, Chin Voon Mukhtar, Gambo Lawal |
| author_facet | Abd Rachman Isnadi, Mohammad Faruq Basir, Rusliza Omenesa, Ramatu Bello Abd Majid, Roslaini Abdullah, Maizaton Atmadini Mat Taib, Che Norma Priya, Sivan Padma Kong, Yong Yean Kin, Chin Voon Mukhtar, Gambo Lawal |
| author_sort | Abd Rachman Isnadi, Mohammad Faruq |
| collection | UPM |
| description | Objective: To determine the involvement and the modulatory effects of IL-33 during Plasmodium berghei ANKA (PbA) infection. Methods: PbA infection in male ICR mice was utilized as a model of malaria. Systemically circulating IL-33 levels were determined in blood plasma by enzyme-linked immunosorbent assay (ELISA). After 24 hours post-inoculation of PbA, recombinant IL-33 and ST2, and antibodies against IL-33 and IgG treatments were administered daily for 3 days. Tissue expression and localization of IL-33 were assessed in organs generally affected by malaria via immunohistochemistry. Moreover, histopathological examination was performed to assess the effects of the treatments. Results: The levels of systemic IL-33 were elevated at the critical phase of PbA infection. Likewise, immunohistochemical analysis revealed a significant upregulation of IL-33 expression at the critical phase in the brain, lungs, and spleen of PbA-infected mice as compared to healthy controls. Treatment with IL-33 protected against experimental cerebral malaria development and reduced pathological features in the brain and lungs of the PbA-infected mice. Conclusions: A potential critical role and involvement of IL-33 in PbA infection may hint at the resolution of immunopathological sequelae associated with malaria. |
| first_indexed | 2024-09-25T03:42:58Z |
| format | Article |
| id | upm.eprints-110277 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-09-25T03:42:58Z |
| publishDate | 2023 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | dspace |
| spelling | upm.eprints-1102772024-07-04T09:04:00Z http://psasir.upm.edu.my/id/eprint/110277/ Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice Abd Rachman Isnadi, Mohammad Faruq Basir, Rusliza Omenesa, Ramatu Bello Abd Majid, Roslaini Abdullah, Maizaton Atmadini Mat Taib, Che Norma Priya, Sivan Padma Kong, Yong Yean Kin, Chin Voon Mukhtar, Gambo Lawal Objective: To determine the involvement and the modulatory effects of IL-33 during Plasmodium berghei ANKA (PbA) infection. Methods: PbA infection in male ICR mice was utilized as a model of malaria. Systemically circulating IL-33 levels were determined in blood plasma by enzyme-linked immunosorbent assay (ELISA). After 24 hours post-inoculation of PbA, recombinant IL-33 and ST2, and antibodies against IL-33 and IgG treatments were administered daily for 3 days. Tissue expression and localization of IL-33 were assessed in organs generally affected by malaria via immunohistochemistry. Moreover, histopathological examination was performed to assess the effects of the treatments. Results: The levels of systemic IL-33 were elevated at the critical phase of PbA infection. Likewise, immunohistochemical analysis revealed a significant upregulation of IL-33 expression at the critical phase in the brain, lungs, and spleen of PbA-infected mice as compared to healthy controls. Treatment with IL-33 protected against experimental cerebral malaria development and reduced pathological features in the brain and lungs of the PbA-infected mice. Conclusions: A potential critical role and involvement of IL-33 in PbA infection may hint at the resolution of immunopathological sequelae associated with malaria. Wolters Kluwer Medknow Publications 2023 Article PeerReviewed Abd Rachman Isnadi, Mohammad Faruq and Basir, Rusliza and Omenesa, Ramatu Bello and Abd Majid, Roslaini and Abdullah, Maizaton Atmadini and Mat Taib, Che Norma and Priya, Sivan Padma and Kong, Yong Yean and Kin, Chin Voon and Mukhtar, Gambo Lawal (2023) Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice. Asian Pacific Journal of Tropical Biomedicine, 13 (12). pp. 521-531. ISSN 2221-1691; ESSN: 2588-9222 https://journals.lww.com/10.4103/2221-1691.391157 10.4103/2221-1691.391157 |
| spellingShingle | Abd Rachman Isnadi, Mohammad Faruq Basir, Rusliza Omenesa, Ramatu Bello Abd Majid, Roslaini Abdullah, Maizaton Atmadini Mat Taib, Che Norma Priya, Sivan Padma Kong, Yong Yean Kin, Chin Voon Mukhtar, Gambo Lawal Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
| title | Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
| title_full | Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
| title_fullStr | Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
| title_full_unstemmed | Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
| title_short | Interleukin-33 exerts pleiotropic immunoregulatory effects in response to Plasmodium berghei ANKA (PbA) infection in mice |
| title_sort | interleukin 33 exerts pleiotropic immunoregulatory effects in response to plasmodium berghei anka pba infection in mice |
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