Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates
Aims: This study was designed to examine the enzyme activity of selected virulent isolates of Ganoderma boninense against oil palm. In a separate in vitro assessment, the effect of macronutrients on the mycelial growth of four selected Ganoderma spp. was also tested. Methodology and results: The stu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Sains Malaysia
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/110452/1/110452.pdf |
| _version_ | 1824452257826996224 |
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| author | Periasamy, Gunashila Yun, Wong Mui Vadamalai, Ganesan Ling, Ho Chai Naidu, Yuvarani Raja Sundram, Shamala |
| author_facet | Periasamy, Gunashila Yun, Wong Mui Vadamalai, Ganesan Ling, Ho Chai Naidu, Yuvarani Raja Sundram, Shamala |
| author_sort | Periasamy, Gunashila |
| collection | UPM |
| description | Aims: This study was designed to examine the enzyme activity of selected virulent isolates of Ganoderma boninense against oil palm. In a separate in vitro assessment, the effect of macronutrients on the mycelial growth of four selected Ganoderma spp. was also tested. Methodology and results: The study involved a comparison of ligninolytic enzymes; lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase (Lac) profiling of eight isolates of G. boninense, categorized into three levels of aggressiveness, with two control isolates (G. boninense PER71 and G. tornatum NPG1) using solid-state fermentation (SSF). The Principal Component Analysis (PCA) revealed that the isolates had a significant production of ligninolytic enzymes on day 80. The most aggressive isolate, ET61 had the highest Lac production. As for the macronutrient test, mycelial growth for all the Ganoderma spp. was highly affected by potassium (K). Conclusion, significance and impact of study: The findings of this study elucidated the characteristics of G. boninense in relation to enzyme production for the degradation of oil palm lignin and the identification of essential nutrients involved in the survival and growth of Ganoderma spp. The study provides vital information on the pathogenic characteristics of G. boninense isolates involved in biomass degradation along with the role of nutrient on the growth of Ganoderma spp. that may influence basal stem rot (BSR) management in the field. |
| first_indexed | 2025-02-19T02:47:39Z |
| format | Article |
| id | upm.eprints-110452 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2025-02-19T02:47:39Z |
| publishDate | 2023 |
| publisher | Universiti Sains Malaysia |
| record_format | dspace |
| spelling | upm.eprints-1104522025-01-24T07:27:51Z http://psasir.upm.edu.my/id/eprint/110452/ Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates Periasamy, Gunashila Yun, Wong Mui Vadamalai, Ganesan Ling, Ho Chai Naidu, Yuvarani Raja Sundram, Shamala Aims: This study was designed to examine the enzyme activity of selected virulent isolates of Ganoderma boninense against oil palm. In a separate in vitro assessment, the effect of macronutrients on the mycelial growth of four selected Ganoderma spp. was also tested. Methodology and results: The study involved a comparison of ligninolytic enzymes; lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase (Lac) profiling of eight isolates of G. boninense, categorized into three levels of aggressiveness, with two control isolates (G. boninense PER71 and G. tornatum NPG1) using solid-state fermentation (SSF). The Principal Component Analysis (PCA) revealed that the isolates had a significant production of ligninolytic enzymes on day 80. The most aggressive isolate, ET61 had the highest Lac production. As for the macronutrient test, mycelial growth for all the Ganoderma spp. was highly affected by potassium (K). Conclusion, significance and impact of study: The findings of this study elucidated the characteristics of G. boninense in relation to enzyme production for the degradation of oil palm lignin and the identification of essential nutrients involved in the survival and growth of Ganoderma spp. The study provides vital information on the pathogenic characteristics of G. boninense isolates involved in biomass degradation along with the role of nutrient on the growth of Ganoderma spp. that may influence basal stem rot (BSR) management in the field. Universiti Sains Malaysia 2023 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/110452/1/110452.pdf Periasamy, Gunashila and Yun, Wong Mui and Vadamalai, Ganesan and Ling, Ho Chai and Naidu, Yuvarani Raja and Sundram, Shamala (2023) Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates. Malaysian Journal of Microbiology, 19 (1). pp. 11-21. ISSN 2231-7538; eISSN: 1823-8262 http://mjm.usm.my/index.php?r=cms/entry/view&id=2710&slug=Ligninolytic-enzymes-profiling-in-association-with-the-aggressiveness-of-Ganoderma-boninense-isolates 10.21161/mjm.221549 |
| spellingShingle | Periasamy, Gunashila Yun, Wong Mui Vadamalai, Ganesan Ling, Ho Chai Naidu, Yuvarani Raja Sundram, Shamala Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates |
| title | Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates |
| title_full | Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates |
| title_fullStr | Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates |
| title_full_unstemmed | Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates |
| title_short | Ligninolytic enzymes profiling in association with the aggressiveness of Ganoderma boninense isolates |
| title_sort | ligninolytic enzymes profiling in association with the aggressiveness of ganoderma boninense isolates |
| url | http://psasir.upm.edu.my/id/eprint/110452/1/110452.pdf |
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