Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected hospitals in Malaysia
Objective: To determine the distribution, phenotypic and genetic background of extended spectrum β-lactamases (ESBL)-producing Klebsiella (K.) pneumoniae clinical isolates associated with K1 and K2 serotypes in two selected hospitals in Malaysia. Methods: A total of 192 K. pneumoniae isolates were c...
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| Format: | Article |
| Language: | English |
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Medknow
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/111167/1/30-38%28APJTM-303-23%295%20final%20proof%2029%20Jan%202024.pdf |
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| author | Anuar, Nurul Syazrah Hazman, Hazmin Jeyakumar, Sharven Raj Mohd Desa, Mohd Nasir Saidi, Hasni Idayu Masri, Siti Norbaya Aziz, Nur Afiza Sulaiman, Nurshahira |
| author_facet | Anuar, Nurul Syazrah Hazman, Hazmin Jeyakumar, Sharven Raj Mohd Desa, Mohd Nasir Saidi, Hasni Idayu Masri, Siti Norbaya Aziz, Nur Afiza Sulaiman, Nurshahira |
| author_sort | Anuar, Nurul Syazrah |
| collection | UPM |
| description | Objective: To determine the distribution, phenotypic and genetic background of extended spectrum β-lactamases (ESBL)-producing Klebsiella (K.) pneumoniae clinical isolates associated with K1 and K2 serotypes in two selected hospitals in Malaysia. Methods: A total of 192 K. pneumoniae isolates were collected and subjected to antibiotic susceptibility, hypermucoviscosity test and multiplex PCR to detect the presence of K1- and K2-serotype associated genes. Multilocus sequence typing (MLST) was performed on ESBL-producing K. pneumoniae isolates presented with K1 and K2 serotypes, followed by phylogenetic analysis. Results: A total of 87 out of 192 (45.3%) of the K. pneumoniae
isolates collected were ESBL producers. However, only 8.3% (16/192) and 10.9% (21/192) of the total isolates were detected to carry K1- and K2-serotype associated genes, respectively. Statistical analysis showed that K1 and K2 capsular serotypes were not significantly associated with ESBL phenotype (P=0.196). However, they were significantly associated with hypervirulent, as demonstrated by the positive string test (P<0.001). MLST analysis revealed that ST23 as the predominant sequence type (ST) in the K1 serotype, while the ST in the K2 serotype is more diverse. Conclusions: Although the occurrence of ESBL-producing isolates among the hypervirulent strains was low, their coexistence warrants the need for continuous surveillance. MLST showed that these isolates were genetically heterogeneous. |
| first_indexed | 2024-09-25T03:43:54Z |
| format | Article |
| id | upm.eprints-111167 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-09-25T03:43:54Z |
| publishDate | 2024 |
| publisher | Medknow |
| record_format | dspace |
| spelling | upm.eprints-1111672024-06-22T15:03:33Z http://psasir.upm.edu.my/id/eprint/111167/ Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected hospitals in Malaysia Anuar, Nurul Syazrah Hazman, Hazmin Jeyakumar, Sharven Raj Mohd Desa, Mohd Nasir Saidi, Hasni Idayu Masri, Siti Norbaya Aziz, Nur Afiza Sulaiman, Nurshahira Objective: To determine the distribution, phenotypic and genetic background of extended spectrum β-lactamases (ESBL)-producing Klebsiella (K.) pneumoniae clinical isolates associated with K1 and K2 serotypes in two selected hospitals in Malaysia. Methods: A total of 192 K. pneumoniae isolates were collected and subjected to antibiotic susceptibility, hypermucoviscosity test and multiplex PCR to detect the presence of K1- and K2-serotype associated genes. Multilocus sequence typing (MLST) was performed on ESBL-producing K. pneumoniae isolates presented with K1 and K2 serotypes, followed by phylogenetic analysis. Results: A total of 87 out of 192 (45.3%) of the K. pneumoniae isolates collected were ESBL producers. However, only 8.3% (16/192) and 10.9% (21/192) of the total isolates were detected to carry K1- and K2-serotype associated genes, respectively. Statistical analysis showed that K1 and K2 capsular serotypes were not significantly associated with ESBL phenotype (P=0.196). However, they were significantly associated with hypervirulent, as demonstrated by the positive string test (P<0.001). MLST analysis revealed that ST23 as the predominant sequence type (ST) in the K1 serotype, while the ST in the K2 serotype is more diverse. Conclusions: Although the occurrence of ESBL-producing isolates among the hypervirulent strains was low, their coexistence warrants the need for continuous surveillance. MLST showed that these isolates were genetically heterogeneous. Medknow 2024-01-29 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111167/1/30-38%28APJTM-303-23%295%20final%20proof%2029%20Jan%202024.pdf Anuar, Nurul Syazrah and Hazman, Hazmin and Jeyakumar, Sharven Raj and Mohd Desa, Mohd Nasir and Saidi, Hasni Idayu and Masri, Siti Norbaya and Aziz, Nur Afiza and Sulaiman, Nurshahira (2024) Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected hospitals in Malaysia. Asian Pacific Journal of Tropical Medicine, 17 (1). pp. 30-38. ISSN 1995-7645; ESSN: 2352-4146 https://journals.lww.com/aptm/fulltext/2024/17010/occurrence_of_k1_and_k2_serotypes_and_genotypic.5.aspx Human body - Microbiology 10.4103/apjtm.apjtm_303_23 |
| spellingShingle | Human body - Microbiology Anuar, Nurul Syazrah Hazman, Hazmin Jeyakumar, Sharven Raj Mohd Desa, Mohd Nasir Saidi, Hasni Idayu Masri, Siti Norbaya Aziz, Nur Afiza Sulaiman, Nurshahira Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected hospitals in Malaysia |
| title | Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected
hospitals in Malaysia |
| title_full | Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected
hospitals in Malaysia |
| title_fullStr | Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected
hospitals in Malaysia |
| title_full_unstemmed | Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected
hospitals in Malaysia |
| title_short | Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected
hospitals in Malaysia |
| title_sort | occurrence of k1 and k2 serotypes and genotypic characteristics of extended spectrum β lactamases producing klebsiella pneumoniae isolated from selected hospitals in malaysia |
| topic | Human body - Microbiology |
| url | http://psasir.upm.edu.my/id/eprint/111167/1/30-38%28APJTM-303-23%295%20final%20proof%2029%20Jan%202024.pdf |
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