Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth
Introduction: Plant growth-promoting rhizobacteria (PGPR) can increase plant growth and encapsulation of PGPR with biochar ensures the viability and survival of PGPR. However, this approach is still underexplored. Objectives: The objectives of this study: 1) to isolate and select a potential PGPR...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/111287/1/Syaziana%20et%20al.%202024.pdf |
| _version_ | 1811138047289327616 |
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| author | Syaziana, N. Siti Anis Othman, Nur Maizatul Idayu Shamsuddin, Aida Soraya Ali, Tan Kee Zuan Mansor, Musliyana |
| author_facet | Syaziana, N. Siti Anis Othman, Nur Maizatul Idayu Shamsuddin, Aida Soraya Ali, Tan Kee Zuan Mansor, Musliyana |
| author_sort | Syaziana, N. Siti Anis |
| collection | UPM |
| description | Introduction: Plant growth-promoting rhizobacteria (PGPR) can increase plant growth and encapsulation of PGPR
with biochar ensures the viability and survival of PGPR. However, this approach is still underexplored.
Objectives: The objectives of this study: 1) to isolate and select a potential PGPR from rice rhizosphere based on
plant growth-promoting characterization, 2) encapsulate the selected PGPR strain SA-10 using biochar and sodium
alginate, and 3) assess the effect of encapsulated and non-encapsulated SA-10 on chili plant growth
compared to a non-inoculated SA-10 and control.
Methodology: SA-10 was isolated from rice rhizosphere and characterized for plant growth promoting traits
including, the nitrogen fixation, phosphate solubilization, production of siderophore, production of indole acetic
acid and production of cellulose degradation enzyme. SA-10 was encapsulated in alginate beads along with
biochar. A pot experiment conducted using encapsulated SA-10, non-encapsulated SA-10, and a control group to
assess the effects of encapsulated SA-10 application on chili plants using complete randomized design (CRD)
experimental design with four replications.
Results: About 28 bacteria isolates were isolated from the rhizosphere and endospheric of rice crops to determine
their effects on the growth of chili plants. The isolates were characterized with zinc, phosphate, and potassium
solubilization, siderophores production, and cellulose degradation. The findings showed isolate SA-10 solubilized
zinc at a rate of 41.6 %, phosphate at 50 %, and potassium at 76.9 %. It also fixed nitrogen, produced siderophores
and degraded cellulose. The isolate also produced the highest IAA at 4.007 mg/L. Isolate SA-10 was
then encapsulated in a mixture of biochar and sodium alginate. The results showed that plants treated with
encapsulated SA-10 had significantly higher leaf area (3.68 cm2), leaf diameter (6.55 cm), and dry weight (0.39
g) compared to those treated with non-encapsulated and the control treatment. Through 16S rRNA gene
sequencing, it was identified that SA-10 belongs to the Enterobacter genus, closely resembling Enterobacter
chuandaensis.
Conclusion: The findings suggest that Enterobacter sp. SA-10 enhances plant growth, and the use of encapsulated
SA-10 with biochar contributes to the growth of chili plants. The encapsulated Enterobacter sp. SA-10 has a
promising potential in enhancing crop yield production and promoting sustainable agriculture. |
| first_indexed | 2024-09-25T03:43:59Z |
| format | Article |
| id | upm.eprints-111287 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-09-25T03:43:59Z |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-1112872024-06-27T13:59:33Z http://psasir.upm.edu.my/id/eprint/111287/ Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth Syaziana, N. Siti Anis Othman, Nur Maizatul Idayu Shamsuddin, Aida Soraya Ali, Tan Kee Zuan Mansor, Musliyana Introduction: Plant growth-promoting rhizobacteria (PGPR) can increase plant growth and encapsulation of PGPR with biochar ensures the viability and survival of PGPR. However, this approach is still underexplored. Objectives: The objectives of this study: 1) to isolate and select a potential PGPR from rice rhizosphere based on plant growth-promoting characterization, 2) encapsulate the selected PGPR strain SA-10 using biochar and sodium alginate, and 3) assess the effect of encapsulated and non-encapsulated SA-10 on chili plant growth compared to a non-inoculated SA-10 and control. Methodology: SA-10 was isolated from rice rhizosphere and characterized for plant growth promoting traits including, the nitrogen fixation, phosphate solubilization, production of siderophore, production of indole acetic acid and production of cellulose degradation enzyme. SA-10 was encapsulated in alginate beads along with biochar. A pot experiment conducted using encapsulated SA-10, non-encapsulated SA-10, and a control group to assess the effects of encapsulated SA-10 application on chili plants using complete randomized design (CRD) experimental design with four replications. Results: About 28 bacteria isolates were isolated from the rhizosphere and endospheric of rice crops to determine their effects on the growth of chili plants. The isolates were characterized with zinc, phosphate, and potassium solubilization, siderophores production, and cellulose degradation. The findings showed isolate SA-10 solubilized zinc at a rate of 41.6 %, phosphate at 50 %, and potassium at 76.9 %. It also fixed nitrogen, produced siderophores and degraded cellulose. The isolate also produced the highest IAA at 4.007 mg/L. Isolate SA-10 was then encapsulated in a mixture of biochar and sodium alginate. The results showed that plants treated with encapsulated SA-10 had significantly higher leaf area (3.68 cm2), leaf diameter (6.55 cm), and dry weight (0.39 g) compared to those treated with non-encapsulated and the control treatment. Through 16S rRNA gene sequencing, it was identified that SA-10 belongs to the Enterobacter genus, closely resembling Enterobacter chuandaensis. Conclusion: The findings suggest that Enterobacter sp. SA-10 enhances plant growth, and the use of encapsulated SA-10 with biochar contributes to the growth of chili plants. The encapsulated Enterobacter sp. SA-10 has a promising potential in enhancing crop yield production and promoting sustainable agriculture. Elsevier 2024-04-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111287/1/Syaziana%20et%20al.%202024.pdf Syaziana, N. Siti Anis and Othman, Nur Maizatul Idayu and Shamsuddin, Aida Soraya and Ali, Tan Kee Zuan and Mansor, Musliyana (2024) Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth. Journal of King Saud University - Science, 36 (6). pp. 1-7. ISSN 1018-3647; ESSN: 2213-686X https://www.sciencedirect.com/science/article/pii/S1018364724001095?via%3Dihub 10.1016/j.jksus.2024.103197 |
| spellingShingle | Syaziana, N. Siti Anis Othman, Nur Maizatul Idayu Shamsuddin, Aida Soraya Ali, Tan Kee Zuan Mansor, Musliyana Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth |
| title | Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth |
| title_full | Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth |
| title_fullStr | Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth |
| title_full_unstemmed | Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth |
| title_short | Isolation and characterization of encapsulated plant growth-promoting Enterobacter sp. SA10 for enhancing chili growth |
| title_sort | isolation and characterization of encapsulated plant growth promoting enterobacter sp sa10 for enhancing chili growth |
| url | http://psasir.upm.edu.my/id/eprint/111287/1/Syaziana%20et%20al.%202024.pdf |
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