Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens
The genetic diversity of 100 Malaysian native chickens was investigated using polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) for two candidate genes: inducible nitric oxide synthase (INOS) and natural resistance-associated macrophage protein 1 (NRAMP1). The two genes were sel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11130/1/11130.pdf |
| _version_ | 1825944865000652800 |
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| author | Tohidi, Reza Idris, Ismail Panandam, Jothi Malar Bejo, Mohd Hair |
| author_facet | Tohidi, Reza Idris, Ismail Panandam, Jothi Malar Bejo, Mohd Hair |
| author_sort | Tohidi, Reza |
| collection | UPM |
| description | The genetic diversity of 100 Malaysian native chickens was investigated using polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) for two candidate genes: inducible nitric oxide synthase (INOS) and natural resistance-associated macrophage protein 1 (NRAMP1). The two genes were selected because of their important role in chicken's immune system. INOS and NRAMP1 PCR products were digested by AluI and SacI restriction enzymes, respectively. The restriction digests produced fragment sizes of 322 and 173 bp for INOS and 722 and 79 bp for NRAMP1 as one allele and an undigested PCR product as the other allele. Both loci were polymorph, however only INOS gene showed Hardy-Weinberg equilibrium. Average heterozygosity and the Shannon information index (I) was 0.43 and 0.62 for INOS and 0.48 and 0.68 for NRAMP1 genes, respectively. The observed polymorphism in this study shows the ability of these candidate genes in marker assisted selection and introgression programs to increase resistance to diseases in both Malaysian native and commercial chickens. |
| first_indexed | 2024-03-06T07:22:36Z |
| format | Article |
| id | upm.eprints-11130 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:22:36Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-111302017-11-13T04:14:05Z http://psasir.upm.edu.my/id/eprint/11130/ Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens Tohidi, Reza Idris, Ismail Panandam, Jothi Malar Bejo, Mohd Hair The genetic diversity of 100 Malaysian native chickens was investigated using polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP) for two candidate genes: inducible nitric oxide synthase (INOS) and natural resistance-associated macrophage protein 1 (NRAMP1). The two genes were selected because of their important role in chicken's immune system. INOS and NRAMP1 PCR products were digested by AluI and SacI restriction enzymes, respectively. The restriction digests produced fragment sizes of 322 and 173 bp for INOS and 722 and 79 bp for NRAMP1 as one allele and an undigested PCR product as the other allele. Both loci were polymorph, however only INOS gene showed Hardy-Weinberg equilibrium. Average heterozygosity and the Shannon information index (I) was 0.43 and 0.62 for INOS and 0.48 and 0.68 for NRAMP1 genes, respectively. The observed polymorphism in this study shows the ability of these candidate genes in marker assisted selection and introgression programs to increase resistance to diseases in both Malaysian native and commercial chickens. Academic Journals 2011-02-21 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11130/1/11130.pdf Tohidi, Reza and Idris, Ismail and Panandam, Jothi Malar and Bejo, Mohd Hair (2011) Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens. African Journal of Biotechnology, 10 (8). art. no. 89EB5BE25532. pp. 1285-1289. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/89EB5BE25532 10.5897/AJB10.829 |
| spellingShingle | Tohidi, Reza Idris, Ismail Panandam, Jothi Malar Bejo, Mohd Hair Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens |
| title | Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens |
| title_full | Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens |
| title_fullStr | Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens |
| title_full_unstemmed | Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens |
| title_short | Analysis of genetic variation of inducible nitric oxide synthase and natural resistance-associated macrophage protein 1 loci in Malaysian native chickens |
| title_sort | analysis of genetic variation of inducible nitric oxide synthase and natural resistance associated macrophage protein 1 loci in malaysian native chickens |
| url | http://psasir.upm.edu.my/id/eprint/11130/1/11130.pdf |
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