Synthesis of fatty esters by polyethylene glycol‐modified lipase
Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEG–nitrophenyl carbonates (activated PEG) with high yield (96–98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of acti...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Wiley
1995
|
| _version_ | 1825940043559075840 |
|---|---|
| author | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin A. Razak, Che Nyonya Salleh, A. Bakar |
| author_facet | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin A. Razak, Che Nyonya Salleh, A. Bakar |
| author_sort | Basri, Mahiran |
| collection | UPM |
| description | Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEG–nitrophenyl carbonates (activated PEG) with high yield (96–98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of activated PEG to the enzyme increased the degree of lipase modification. These modified lipases exhibited specific ester synthesis activities on organic solvents compared with native lipase. The degree of activity enhancement depended on the size of activated PEG used and the degree of modification of the enzyme. Maximal activity was attained after exhaustive of modification. The effects of different solvents, reaction temperature, and fatty acids on the esterification activity and the stability of the modified enzyme were investigated. The optimum esterification temperature (40° C) and preference of fatty acids as acyl donors of the modified lipase were very similar to those of the native enzyme. The modified lipase exhibited higher activity non-polar solvents than in polar solvents, and showed higher temperature, solvent and storage stability then the native lipase. |
| first_indexed | 2025-03-07T12:55:22Z |
| format | Article |
| id | upm.eprints-111859 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-03-07T12:55:22Z |
| publishDate | 1995 |
| publisher | Wiley |
| record_format | dspace |
| spelling | upm.eprints-1118592025-03-03T04:44:13Z http://psasir.upm.edu.my/id/eprint/111859/ Synthesis of fatty esters by polyethylene glycol‐modified lipase Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin A. Razak, Che Nyonya Salleh, A. Bakar Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEG–nitrophenyl carbonates (activated PEG) with high yield (96–98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of activated PEG to the enzyme increased the degree of lipase modification. These modified lipases exhibited specific ester synthesis activities on organic solvents compared with native lipase. The degree of activity enhancement depended on the size of activated PEG used and the degree of modification of the enzyme. Maximal activity was attained after exhaustive of modification. The effects of different solvents, reaction temperature, and fatty acids on the esterification activity and the stability of the modified enzyme were investigated. The optimum esterification temperature (40° C) and preference of fatty acids as acyl donors of the modified lipase were very similar to those of the native enzyme. The modified lipase exhibited higher activity non-polar solvents than in polar solvents, and showed higher temperature, solvent and storage stability then the native lipase. Wiley 1995 Article PeerReviewed Basri, Mahiran and Ampon, Kamaruzaman and Wan Yunus, Wan Md. Zin and A. Razak, Che Nyonya and Salleh, A. Bakar (1995) Synthesis of fatty esters by polyethylene glycol‐modified lipase. Journal of Chemical Technology & Biotechnology, 64 (1). pp. 10-16. ISSN 0268-2575; eISSN: 1097-4660 https://onlinelibrary.wiley.com/doi/10.1002/jctb.280640103 10.1002/jctb.280640103 |
| spellingShingle | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin A. Razak, Che Nyonya Salleh, A. Bakar Synthesis of fatty esters by polyethylene glycol‐modified lipase |
| title | Synthesis of fatty esters by polyethylene glycol‐modified lipase |
| title_full | Synthesis of fatty esters by polyethylene glycol‐modified lipase |
| title_fullStr | Synthesis of fatty esters by polyethylene glycol‐modified lipase |
| title_full_unstemmed | Synthesis of fatty esters by polyethylene glycol‐modified lipase |
| title_short | Synthesis of fatty esters by polyethylene glycol‐modified lipase |
| title_sort | synthesis of fatty esters by polyethylene glycol modified lipase |
| work_keys_str_mv | AT basrimahiran synthesisoffattyestersbypolyethyleneglycolmodifiedlipase AT amponkamaruzaman synthesisoffattyestersbypolyethyleneglycolmodifiedlipase AT wanyunuswanmdzin synthesisoffattyestersbypolyethyleneglycolmodifiedlipase AT arazakchenyonya synthesisoffattyestersbypolyethyleneglycolmodifiedlipase AT sallehabakar synthesisoffattyestersbypolyethyleneglycolmodifiedlipase |