Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock

The effects of genetic manipulation of eggs and sperm on the yields of gynogenetic fry of Puntius gonionotus were investigated. Gynogenesis was achieved by cold-and heat-shocking eggs fertilized with ultraviolet irradiated sperm of P. schwanenfeldii at various times after fertilization and at differ...

Full description

Bibliographic Details
Main Authors: Siraj, Siti Shapor, Seki, Shingo, Jee, Ang Kok, Yamada, Yukio, Taniguchi, Nobuhiko
Format: Article
Language:English
Published: The Japanese Society of Fisheries Science 1993
Online Access:http://psasir.upm.edu.my/id/eprint/111927/1/111927.pdf
_version_ 1825940047373795328
author Siraj, Siti Shapor
Seki, Shingo
Jee, Ang Kok
Yamada, Yukio
Taniguchi, Nobuhiko
author_facet Siraj, Siti Shapor
Seki, Shingo
Jee, Ang Kok
Yamada, Yukio
Taniguchi, Nobuhiko
author_sort Siraj, Siti Shapor
collection UPM
description The effects of genetic manipulation of eggs and sperm on the yields of gynogenetic fry of Puntius gonionotus were investigated. Gynogenesis was achieved by cold-and heat-shocking eggs fertilized with ultraviolet irradiated sperm of P. schwanenfeldii at various times after fertilization and at different duration intervals. 66.6% viable, gynogenetic fry were obtained when eggs were inseminated with irradiated sperm and cold shocked at 2°C for 5 minutes duration 1 minute after fertilization. At warm water temperature shocks the fertilized eggs performed best at 42°C, with percent survival rates of 20.0% 1 minute after fertilization for 1.0 minute duration and 17.2% and 14.7% 13 and 23 minutes after fertilization respectively for a duration of 1.5 minutes.
first_indexed 2024-09-25T03:44:40Z
format Article
id upm.eprints-111927
institution Universiti Putra Malaysia
language English
last_indexed 2024-09-25T03:44:40Z
publishDate 1993
publisher The Japanese Society of Fisheries Science
record_format dspace
spelling upm.eprints-1119272024-09-11T08:21:38Z http://psasir.upm.edu.my/id/eprint/111927/ Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock Siraj, Siti Shapor Seki, Shingo Jee, Ang Kok Yamada, Yukio Taniguchi, Nobuhiko The effects of genetic manipulation of eggs and sperm on the yields of gynogenetic fry of Puntius gonionotus were investigated. Gynogenesis was achieved by cold-and heat-shocking eggs fertilized with ultraviolet irradiated sperm of P. schwanenfeldii at various times after fertilization and at different duration intervals. 66.6% viable, gynogenetic fry were obtained when eggs were inseminated with irradiated sperm and cold shocked at 2°C for 5 minutes duration 1 minute after fertilization. At warm water temperature shocks the fertilized eggs performed best at 42°C, with percent survival rates of 20.0% 1 minute after fertilization for 1.0 minute duration and 17.2% and 14.7% 13 and 23 minutes after fertilization respectively for a duration of 1.5 minutes. The Japanese Society of Fisheries Science 1993 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111927/1/111927.pdf Siraj, Siti Shapor and Seki, Shingo and Jee, Ang Kok and Yamada, Yukio and Taniguchi, Nobuhiko (1993) Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock. Nippon Suisan Gakkaishi, 59 (6). pp. 957-962. ISSN 0021-5392 https://www.jstage.jst.go.jp/article/suisan1932/59/6/59_6_957/_article 10.2331/suisan.59.957
spellingShingle Siraj, Siti Shapor
Seki, Shingo
Jee, Ang Kok
Yamada, Yukio
Taniguchi, Nobuhiko
Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock
title Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock
title_full Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock
title_fullStr Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock
title_full_unstemmed Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock
title_short Diploid Gynogenesis in Lampam Jawa Puntius gonionotus using UV irradiated sperm of Puntius schwanenfeldii followed by temperature shock
title_sort diploid gynogenesis in lampam jawa puntius gonionotus using uv irradiated sperm of puntius schwanenfeldii followed by temperature shock
url http://psasir.upm.edu.my/id/eprint/111927/1/111927.pdf
work_keys_str_mv AT sirajsitishapor diploidgynogenesisinlampamjawapuntiusgonionotususinguvirradiatedspermofpuntiusschwanenfeldiifollowedbytemperatureshock
AT sekishingo diploidgynogenesisinlampamjawapuntiusgonionotususinguvirradiatedspermofpuntiusschwanenfeldiifollowedbytemperatureshock
AT jeeangkok diploidgynogenesisinlampamjawapuntiusgonionotususinguvirradiatedspermofpuntiusschwanenfeldiifollowedbytemperatureshock
AT yamadayukio diploidgynogenesisinlampamjawapuntiusgonionotususinguvirradiatedspermofpuntiusschwanenfeldiifollowedbytemperatureshock
AT taniguchinobuhiko diploidgynogenesisinlampamjawapuntiusgonionotususinguvirradiatedspermofpuntiusschwanenfeldiifollowedbytemperatureshock