Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4
Several thermostable proteases have been identified, yet only a handful have undergone the processes of cloning, comprehensive characterization, and full exploitation in various industrial applications. Our primary aim in this study was to clone a thermostable alkaline protease from a thermophilic b...
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| Format: | Article |
| Language: | English |
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Korean Society for Microbiolog and Biotechnology
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/112121/1/jmb-34-2-436.pdf |
| _version_ | 1825940076458147840 |
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| author | Allison, Suleiman D AdeelaYasid, Nur Shariff, Fairolniza Mohd Abdul Rahman, Nor’Aini |
| author_facet | Allison, Suleiman D AdeelaYasid, Nur Shariff, Fairolniza Mohd Abdul Rahman, Nor’Aini |
| author_sort | Allison, Suleiman D |
| collection | UPM |
| description | Several thermostable proteases have been identified, yet only a handful have undergone the processes of cloning, comprehensive characterization, and full exploitation in various industrial applications. Our primary aim in this study was to clone a thermostable alkaline protease from a thermophilic bacterium and assess its potential for use in various industries. The research involved the amplification of the SpSKF4 protease gene, a thermostable alkaline serine protease obtained from the Geobacillus thermoglucosidasius SKF4 bacterium through polymerase chain reaction (PCR). The purified recombinant SpSKF4 protease was characterized, followed by evaluation of its possible industrial applications. The analysis of the gene sequence revealed an open reading frame (ORF) consisting of 1,206 bp, coding for a protein containing 401 amino acids. The cloned gene was expressed in Escherichia coli. The molecular weight of the enzyme was measured at 28 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The partially purified enzyme has its highest activity at a pH of 10 and a temperature of 80°C. In addition, the enzyme showed a half-life of 15 h at 80°C, and there was a 60% increase in its activity at 10 mM Ca2+ concentration. The activity of the protease was completely inhibited (100%) by phenylmethylsulfonyl fluoride (PMSF); however, the addition of sodium dodecyl sulfate (SDS) resulted in a 20% increase in activity. The enzyme was also stable in various organic solvents and in certain commercial detergents. Furthermore, the enzyme exhibited strong potential for industrial use, particularly as a detergent additive and for facilitating the recovery of silver from X-ray film. |
| first_indexed | 2024-12-09T02:23:15Z |
| format | Article |
| id | upm.eprints-112121 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-12-09T02:23:15Z |
| publishDate | 2024 |
| publisher | Korean Society for Microbiolog and Biotechnology |
| record_format | dspace |
| spelling | upm.eprints-1121212024-10-23T03:07:04Z http://psasir.upm.edu.my/id/eprint/112121/ Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4 Allison, Suleiman D AdeelaYasid, Nur Shariff, Fairolniza Mohd Abdul Rahman, Nor’Aini Several thermostable proteases have been identified, yet only a handful have undergone the processes of cloning, comprehensive characterization, and full exploitation in various industrial applications. Our primary aim in this study was to clone a thermostable alkaline protease from a thermophilic bacterium and assess its potential for use in various industries. The research involved the amplification of the SpSKF4 protease gene, a thermostable alkaline serine protease obtained from the Geobacillus thermoglucosidasius SKF4 bacterium through polymerase chain reaction (PCR). The purified recombinant SpSKF4 protease was characterized, followed by evaluation of its possible industrial applications. The analysis of the gene sequence revealed an open reading frame (ORF) consisting of 1,206 bp, coding for a protein containing 401 amino acids. The cloned gene was expressed in Escherichia coli. The molecular weight of the enzyme was measured at 28 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The partially purified enzyme has its highest activity at a pH of 10 and a temperature of 80°C. In addition, the enzyme showed a half-life of 15 h at 80°C, and there was a 60% increase in its activity at 10 mM Ca2+ concentration. The activity of the protease was completely inhibited (100%) by phenylmethylsulfonyl fluoride (PMSF); however, the addition of sodium dodecyl sulfate (SDS) resulted in a 20% increase in activity. The enzyme was also stable in various organic solvents and in certain commercial detergents. Furthermore, the enzyme exhibited strong potential for industrial use, particularly as a detergent additive and for facilitating the recovery of silver from X-ray film. Korean Society for Microbiolog and Biotechnology 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112121/1/jmb-34-2-436.pdf Allison, Suleiman D and AdeelaYasid, Nur and Shariff, Fairolniza Mohd and Abdul Rahman, Nor’Aini (2024) Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4. Journal of Microbiology and Biotechnology, 34 (2). pp. 436-456. ISSN 1017-7825; ESSN: 1738-8872 https://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.2306.06050 10.4014/jmb.2306.06050 |
| spellingShingle | Allison, Suleiman D AdeelaYasid, Nur Shariff, Fairolniza Mohd Abdul Rahman, Nor’Aini Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4 |
| title | Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4 |
| title_full | Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4 |
| title_fullStr | Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4 |
| title_full_unstemmed | Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4 |
| title_short | Molecular cloning, characterization, and application of organic solvent-stable and detergent-compatible thermostable alkaline protease from Geobacillus thermoglucosidasius SKF4 |
| title_sort | molecular cloning characterization and application of organic solvent stable and detergent compatible thermostable alkaline protease from geobacillus thermoglucosidasius skf4 |
| url | http://psasir.upm.edu.my/id/eprint/112121/1/jmb-34-2-436.pdf |
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