Optimization of osmotic shock process variables for enhancement of the release of periplasmic interferon-α2b from Escherichia coli using response surface method
The osmotic shock process for the release of periplasmic recombinant human interferon-α2b from Escherichia coli was optimized using response surface method (RSM). The process parameters such as pH, buffer concentration and sucrose concentration in hypertonic solution, cell concentration to hypertoni...
| Hauptverfasser: | , , , , , |
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| Format: | Artikel |
| Sprache: | English English |
| Veröffentlicht: |
Elsevier
2010
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| Online Zugang: | http://psasir.upm.edu.my/id/eprint/11265/1/Optimization%20of%20osmotic%20shock%20process%20variables%20for%20enhancement%20of%20the%20release%20of%20periplasmic%20interferon.pdf |