Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂
Vibrio alginolyticus is the causative agent of vibriosis, a common bacterial infection in grouper aquaculture that is associated with the development of haemorrhagic and non-haemorrhagic ulcerations on the fish. In the present study, comparative proteome analysis was performed on serum samples from...
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| Format: | Article |
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John Wiley and Sons
2024
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| author | Abu Aziz, Nurhikmah Christianus, Annie Wan Solahudin, Wan Mohd Syazwan Ismail, Intan Safinar Low, Chen‐Fei |
| author_facet | Abu Aziz, Nurhikmah Christianus, Annie Wan Solahudin, Wan Mohd Syazwan Ismail, Intan Safinar Low, Chen‐Fei |
| author_sort | Abu Aziz, Nurhikmah |
| collection | UPM |
| description | Vibrio alginolyticus is the causative agent of vibriosis, a common bacterial infection in grouper aquaculture that is associated with the development of haemorrhagic and non-haemorrhagic ulcerations on the fish. In the present study, comparative proteome analysis was performed on serum samples from Vibrio-resistant and Vibrio-susceptible grouper. Samples were analysed using high-throughput LC-MS/MS and identified 2770 unique peptides that corresponded to 344 proteins. Subsequent analysis identified 21 proteins that were significantly up-regulated in the resistant group compared to the control and the susceptible groups. Those proteins are associated with immunostimulatory effects, signalling and binding cascade, metabolism, and maintaining tissue integrity and physiological condition. Besides, potential protein biomarkers related to the immune system were identified, which could be associated with the disease-resistant phenotype. These data provide insights into the underlying immune mechanism of hybrid groupers upon Vibrio sp. infection. © 2024 John Wiley & Sons Ltd. |
| first_indexed | 2024-12-09T02:24:51Z |
| format | Article |
| id | upm.eprints-112898 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-12-09T02:24:51Z |
| publishDate | 2024 |
| publisher | John Wiley and Sons |
| record_format | dspace |
| spelling | upm.eprints-1128982024-10-28T07:56:34Z http://psasir.upm.edu.my/id/eprint/112898/ Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ Abu Aziz, Nurhikmah Christianus, Annie Wan Solahudin, Wan Mohd Syazwan Ismail, Intan Safinar Low, Chen‐Fei Vibrio alginolyticus is the causative agent of vibriosis, a common bacterial infection in grouper aquaculture that is associated with the development of haemorrhagic and non-haemorrhagic ulcerations on the fish. In the present study, comparative proteome analysis was performed on serum samples from Vibrio-resistant and Vibrio-susceptible grouper. Samples were analysed using high-throughput LC-MS/MS and identified 2770 unique peptides that corresponded to 344 proteins. Subsequent analysis identified 21 proteins that were significantly up-regulated in the resistant group compared to the control and the susceptible groups. Those proteins are associated with immunostimulatory effects, signalling and binding cascade, metabolism, and maintaining tissue integrity and physiological condition. Besides, potential protein biomarkers related to the immune system were identified, which could be associated with the disease-resistant phenotype. These data provide insights into the underlying immune mechanism of hybrid groupers upon Vibrio sp. infection. © 2024 John Wiley & Sons Ltd. John Wiley and Sons 2024 Article PeerReviewed Abu Aziz, Nurhikmah and Christianus, Annie and Wan Solahudin, Wan Mohd Syazwan and Ismail, Intan Safinar and Low, Chen‐Fei (2024) Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂. Journal of Fish Diseases, 47 (7). art. no. e13940. ISSN 0140-7775; eISSN: 1365-2761 https://doi.org/10.1111/jfd.13940 10.1111/jfd.13940 |
| spellingShingle | Abu Aziz, Nurhikmah Christianus, Annie Wan Solahudin, Wan Mohd Syazwan Ismail, Intan Safinar Low, Chen‐Fei Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ |
| title | Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ |
| title_full | Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ |
| title_fullStr | Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ |
| title_full_unstemmed | Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ |
| title_short | Comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in Vibrio‐resistant hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ |
| title_sort | comparative proteome analysis revealed potential biomarkers and the underlying immune mechanisms in vibrio resistant hybrid grouper epinephelus fuscoguttatus ♀ epinephelus lanceolatus ♂ |
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