Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats
A trial was conducted to compare the efficacy of intranasal vaccination in protecting goats against pneumonic pasteurellosis with intramuscular vaccination using an oil adjuvant vaccine, and a combination of the two methods. Forty goats were divided into four equal groups. Group 1 was vaccinated twi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
British Veterinary Associationadminoff@bsava.com
1998
|
| _version_ | 1824452328015527936 |
|---|---|
| author | Effendy, A.W.M. Zamri-Saad, M. Puspa, R. Rosiah, S. |
| author_facet | Effendy, A.W.M. Zamri-Saad, M. Puspa, R. Rosiah, S. |
| author_sort | Effendy, A.W.M. |
| collection | UPM |
| description | A trial was conducted to compare the efficacy of intranasal vaccination in protecting goats against pneumonic pasteurellosis with intramuscular vaccination using an oil adjuvant vaccine, and a combination of the two methods. Forty goats were divided into four equal groups. Group 1 was vaccinated twice intranasally with formalin-killed Pasteurella haemolytica A2, group 2 was vaccinated twice intramuscularly with an oil adjuvant vaccine containing P haemolytica A7, and group 3 was initially vaccinated intranasally with the formalin-killed P haemolytica A2 followed by intramuscular vaccination with the oil adjuvant vaccine. In each group the two vaccinations were carried out four weeks apart. Group 4 was the unvaccinated control group. All goats were challenged intratracheally with 4 ml of an inoculum containing live P haemolytica A2 at a concentration of 1.3 x 107 colony forming units/ml two weeks after the last vaccination and were killed 14 days after the challenge. Although group 2 showed the highest clinical score following the challenge, deaths were observed only in group 3. Three goats in group 1 had pneumonic lung lesions, compared with six goats in group 2 and all the goats in groups 3 and 4. The lung lesions in group 1 were significantly (P < 0.05) less severe than in groups 3 and 4. Similarly, the lesions in group 2 were markedly less severe than in groups 3 and 4, although the differences were not significant. The difference between the extent of the lung lesions in the goats in groups 1 and 2 was not significant. Antibody against P haemolytica A2 in group 1 reached peak levels and was significantly (P < 0.01) higher than in the control group one week after the second vaccination, before declining. |
| first_indexed | 2025-02-19T02:48:46Z |
| format | Article |
| id | upm.eprints-113141 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-02-19T02:48:46Z |
| publishDate | 1998 |
| publisher | British Veterinary Associationadminoff@bsava.com |
| record_format | dspace |
| spelling | upm.eprints-1131412025-02-05T06:53:26Z http://psasir.upm.edu.my/id/eprint/113141/ Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats Effendy, A.W.M. Zamri-Saad, M. Puspa, R. Rosiah, S. A trial was conducted to compare the efficacy of intranasal vaccination in protecting goats against pneumonic pasteurellosis with intramuscular vaccination using an oil adjuvant vaccine, and a combination of the two methods. Forty goats were divided into four equal groups. Group 1 was vaccinated twice intranasally with formalin-killed Pasteurella haemolytica A2, group 2 was vaccinated twice intramuscularly with an oil adjuvant vaccine containing P haemolytica A7, and group 3 was initially vaccinated intranasally with the formalin-killed P haemolytica A2 followed by intramuscular vaccination with the oil adjuvant vaccine. In each group the two vaccinations were carried out four weeks apart. Group 4 was the unvaccinated control group. All goats were challenged intratracheally with 4 ml of an inoculum containing live P haemolytica A2 at a concentration of 1.3 x 107 colony forming units/ml two weeks after the last vaccination and were killed 14 days after the challenge. Although group 2 showed the highest clinical score following the challenge, deaths were observed only in group 3. Three goats in group 1 had pneumonic lung lesions, compared with six goats in group 2 and all the goats in groups 3 and 4. The lung lesions in group 1 were significantly (P < 0.05) less severe than in groups 3 and 4. Similarly, the lesions in group 2 were markedly less severe than in groups 3 and 4, although the differences were not significant. The difference between the extent of the lung lesions in the goats in groups 1 and 2 was not significant. Antibody against P haemolytica A2 in group 1 reached peak levels and was significantly (P < 0.01) higher than in the control group one week after the second vaccination, before declining. British Veterinary Associationadminoff@bsava.com 1998 Article PeerReviewed Effendy, A.W.M. and Zamri-Saad, M. and Puspa, R. and Rosiah, S. (1998) Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats. Veterinary Record, 142 (16). pp. 428-431. ISSN 0042-4900; eISSN: 0042-4900 https://bvajournals.onlinelibrary.wiley.com/doi/10.1136/vr.142.16.428 10.1136/vr.142.16.428 |
| spellingShingle | Effendy, A.W.M. Zamri-Saad, M. Puspa, R. Rosiah, S. Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title | Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_full | Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_fullStr | Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_full_unstemmed | Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_short | Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_sort | efficacy of intranasal administration of formalin killed pasteurella haemolytica a2 against intratracheal challenge in goats |
| work_keys_str_mv | AT effendyawm efficacyofintranasaladministrationofformalinkilledpasteurellahaemolyticaa2againstintratrachealchallengeingoats AT zamrisaadm efficacyofintranasaladministrationofformalinkilledpasteurellahaemolyticaa2againstintratrachealchallengeingoats AT puspar efficacyofintranasaladministrationofformalinkilledpasteurellahaemolyticaa2againstintratrachealchallengeingoats AT rosiahs efficacyofintranasaladministrationofformalinkilledpasteurellahaemolyticaa2againstintratrachealchallengeingoats |