Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation
This study investigates the synthesis of (hemi)cellulolytic enzymes, including endoglucanase (CMCase), xylanase, and β-glucosidase, employing Trichoderma reesei RUT-C30 and deoiled oil palm mesocarp fiber (OPMF) through solid-state fermentation (SSF). The objective was to determine the optimal proce...
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| Format: | Article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113248/1/113248.pdf |
| _version_ | 1825940282779107328 |
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| author | Al-Qassab, Ali Abdulkareem Zakaria, Mohd Rafein Yunus, Robiah Salleh, Mohamad Amran Mohd Mokhtar, Mohd Noriznan |
| author_facet | Al-Qassab, Ali Abdulkareem Zakaria, Mohd Rafein Yunus, Robiah Salleh, Mohamad Amran Mohd Mokhtar, Mohd Noriznan |
| author_sort | Al-Qassab, Ali Abdulkareem |
| collection | UPM |
| description | This study investigates the synthesis of (hemi)cellulolytic enzymes, including endoglucanase (CMCase), xylanase, and β-glucosidase, employing Trichoderma reesei RUT-C30 and deoiled oil palm mesocarp fiber (OPMF) through solid-state fermentation (SSF). The objective was to determine the optimal process conditions for achieving high enzyme activities through a one-factor-at-a-time approach. The study primarily focused on the impact of the solid-to-liquid ratio, incubation period, initial pH, and temperature on enzyme activity. The effects of OPMF pretreatment, particularly deoiling and fortification, were explored. This approach significantly improved enzyme activity levels compared to the initial conditions, with CMCase increasing by 111.6 %, xylanase by 665.2 %, and β-Glucosidase by 1678.1 %. Xylanase and β-glucosidase activities, peaking at 1346.75 and 9.89 IU per gram dry substrate (GDS), respectively, under optimized conditions (1:4 ratio, pH 7.5, 20 °C, 9-day incubation). With lower moisture levels, CMCase reached its maximum activity of 227.84 IU/GDS. The study highlights how important it is for agro-industrial byproducts to support environmentally sustainable practices in the palm oil industry. It also emphasizes how differently each enzyme reacts to changes in process parameters. |
| first_indexed | 2024-12-09T02:25:36Z |
| format | Article |
| id | upm.eprints-113248 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-12-09T02:25:36Z |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-1132482024-11-19T07:41:36Z http://psasir.upm.edu.my/id/eprint/113248/ Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation Al-Qassab, Ali Abdulkareem Zakaria, Mohd Rafein Yunus, Robiah Salleh, Mohamad Amran Mohd Mokhtar, Mohd Noriznan This study investigates the synthesis of (hemi)cellulolytic enzymes, including endoglucanase (CMCase), xylanase, and β-glucosidase, employing Trichoderma reesei RUT-C30 and deoiled oil palm mesocarp fiber (OPMF) through solid-state fermentation (SSF). The objective was to determine the optimal process conditions for achieving high enzyme activities through a one-factor-at-a-time approach. The study primarily focused on the impact of the solid-to-liquid ratio, incubation period, initial pH, and temperature on enzyme activity. The effects of OPMF pretreatment, particularly deoiling and fortification, were explored. This approach significantly improved enzyme activity levels compared to the initial conditions, with CMCase increasing by 111.6 %, xylanase by 665.2 %, and β-Glucosidase by 1678.1 %. Xylanase and β-glucosidase activities, peaking at 1346.75 and 9.89 IU per gram dry substrate (GDS), respectively, under optimized conditions (1:4 ratio, pH 7.5, 20 °C, 9-day incubation). With lower moisture levels, CMCase reached its maximum activity of 227.84 IU/GDS. The study highlights how important it is for agro-industrial byproducts to support environmentally sustainable practices in the palm oil industry. It also emphasizes how differently each enzyme reacts to changes in process parameters. Elsevier 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/113248/1/113248.pdf Al-Qassab, Ali Abdulkareem and Zakaria, Mohd Rafein and Yunus, Robiah and Salleh, Mohamad Amran Mohd and Mokhtar, Mohd Noriznan (2024) Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation. International Journal of Biological Macromolecules, 276 (pt.2). art. no. 134030. pp. 1-11. ISSN 0141-8130; eISSN: 1879-0003 https://www.sciencedirect.com/science/article/pii/S0141813024048359 Enzymes - Solid state fermentation - Case studies Bioprocess Engineering 10.1016/j.ijbiomac.2024.134030 |
| spellingShingle | Enzymes - Solid state fermentation - Case studies Bioprocess Engineering Al-Qassab, Ali Abdulkareem Zakaria, Mohd Rafein Yunus, Robiah Salleh, Mohamad Amran Mohd Mokhtar, Mohd Noriznan Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation |
| title | Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation |
| title_full | Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation |
| title_fullStr | Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation |
| title_full_unstemmed | Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation |
| title_short | Investigating process parameters to enhance (hemi)cellulolytic enzymes activity produced by Trichoderma reesei RUT-C30 using deoiled oil palm mesocarp fiber in solid-state fermentation |
| title_sort | investigating process parameters to enhance hemi cellulolytic enzymes activity produced by trichoderma reesei rut c30 using deoiled oil palm mesocarp fiber in solid state fermentation |
| topic | Enzymes - Solid state fermentation - Case studies Bioprocess Engineering |
| url | http://psasir.upm.edu.my/id/eprint/113248/1/113248.pdf |
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