Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages
Mud lobsters are crustaceans from the genus Thalassina which are lesser known and seldom seen but are nevertheless an important organism to the mangrove ecosystem. In Malaysia and Thailand, mud lobsters are eaten by locals as treatment for asthma. It is traditionally believed that they are effective...
| Príomhchruthaitheoirí: | , , , , |
|---|---|
| Formáid: | Alt |
| Foilsithe / Cruthaithe: |
Universiti Sains Malaysia
2021
|
| Ábhair: |
| _version_ | 1825940284894085120 |
|---|---|
| author | Zakaria, Nur Nadiah Masnindah, Malahubban Fakurazi, Sharida Wong, Sie Chuong Rajaee, Amy Halimah |
| author_facet | Zakaria, Nur Nadiah Masnindah, Malahubban Fakurazi, Sharida Wong, Sie Chuong Rajaee, Amy Halimah |
| author_sort | Zakaria, Nur Nadiah |
| collection | UPM |
| description | Mud lobsters are crustaceans from the genus Thalassina which are lesser known and seldom seen but are nevertheless an important organism to the mangrove ecosystem. In Malaysia and Thailand, mud lobsters are eaten by locals as treatment for asthma. It is traditionally believed that they are effective in reducing the number of asthma attacks and severity of asthma symptoms. However, the therapeutic potential of mud lobster extract remains unclear and has not been fully elucidated or reported in any scientic study. The objectives of this study are to investigate the anti-inammatory potential of mud lobster, Thalassina anomala extracts (hexane, chloroform and methanol) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, and to identify the potential bioactive compounds involved. An MTT assay was performed to determine the cytotoxicity of the T. anomala extracts on RAW 264.7 macrophages. Nitrite quantication assay and enzyme-linked immunosorbent assay (ELISA) were conducted to investigate the ability of the T. anomala extracts to suppress the secretion and expression of nitric oxide (NO), Prostaglandin E2 (PGE2) and proinammatory cytokines (TNF-α, IL-6 and IL-1β) in LPS-stimulated macrophages. GC-MS analysis was done to identify putative metabolites. The hexane extract of T. anomala showed anti-inammatory activity by signicantly inhibiting the LPS-induced production of NO, PGE2, interleukin- (IL-) 6, IL-1β and tumour necrosis factor-alpha (TNF-α) in a concentration-dependent manner. Hexane extract treatment with 100 µg/mL has decreased the NO secretion into 37 µM. Meanwhile, hexane extract at concentration of 100 µg/mL able to signicantly suppressed PGE2,TNF-α, IL-6 and IL-1β production into 2015 pg/mL, 2406 pg/mL, 460 pg/mL and 9.6 pg/mL, respectively. GC-MS analysis of the hexane extract revealed the presence of 19 putative compounds. The identied compounds were reported to have anti-inammatory, antioxidant and antibacterial activities. These results suggest that the hexane extract of T. anomala potentially has anti-inammatory properties and concentration dependently suppressed NO, PGE2 and proinammatory cytokines’ production in LPS-stimulated macrophages. The ndings provide a rational basis of the traditional use of mud lobster for inammation-associated ailments |
| first_indexed | 2024-12-09T02:25:38Z |
| format | Article |
| id | upm.eprints-113258 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-12-09T02:25:38Z |
| publishDate | 2021 |
| publisher | Universiti Sains Malaysia |
| record_format | dspace |
| spelling | upm.eprints-1132582024-11-22T01:56:32Z http://psasir.upm.edu.my/id/eprint/113258/ Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages Zakaria, Nur Nadiah Masnindah, Malahubban Fakurazi, Sharida Wong, Sie Chuong Rajaee, Amy Halimah Mud lobsters are crustaceans from the genus Thalassina which are lesser known and seldom seen but are nevertheless an important organism to the mangrove ecosystem. In Malaysia and Thailand, mud lobsters are eaten by locals as treatment for asthma. It is traditionally believed that they are effective in reducing the number of asthma attacks and severity of asthma symptoms. However, the therapeutic potential of mud lobster extract remains unclear and has not been fully elucidated or reported in any scientic study. The objectives of this study are to investigate the anti-inammatory potential of mud lobster, Thalassina anomala extracts (hexane, chloroform and methanol) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, and to identify the potential bioactive compounds involved. An MTT assay was performed to determine the cytotoxicity of the T. anomala extracts on RAW 264.7 macrophages. Nitrite quantication assay and enzyme-linked immunosorbent assay (ELISA) were conducted to investigate the ability of the T. anomala extracts to suppress the secretion and expression of nitric oxide (NO), Prostaglandin E2 (PGE2) and proinammatory cytokines (TNF-α, IL-6 and IL-1β) in LPS-stimulated macrophages. GC-MS analysis was done to identify putative metabolites. The hexane extract of T. anomala showed anti-inammatory activity by signicantly inhibiting the LPS-induced production of NO, PGE2, interleukin- (IL-) 6, IL-1β and tumour necrosis factor-alpha (TNF-α) in a concentration-dependent manner. Hexane extract treatment with 100 µg/mL has decreased the NO secretion into 37 µM. Meanwhile, hexane extract at concentration of 100 µg/mL able to signicantly suppressed PGE2,TNF-α, IL-6 and IL-1β production into 2015 pg/mL, 2406 pg/mL, 460 pg/mL and 9.6 pg/mL, respectively. GC-MS analysis of the hexane extract revealed the presence of 19 putative compounds. The identied compounds were reported to have anti-inammatory, antioxidant and antibacterial activities. These results suggest that the hexane extract of T. anomala potentially has anti-inammatory properties and concentration dependently suppressed NO, PGE2 and proinammatory cytokines’ production in LPS-stimulated macrophages. The ndings provide a rational basis of the traditional use of mud lobster for inammation-associated ailments Universiti Sains Malaysia 2021 Article PeerReviewed Zakaria, Nur Nadiah and Masnindah, Malahubban and Fakurazi, Sharida and Wong, Sie Chuong and Rajaee, Amy Halimah (2021) Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages. Tropical Life Sciences Research, 32 (1). pp. 143-160. https://ejournal.usm.my/tlsr/article/view/tlsr_vol32-no-1-2021_9/pdf crustacean Cell culture Aquaculture 10.21315/tlsr2021.32.1.9 |
| spellingShingle | crustacean Cell culture Aquaculture Zakaria, Nur Nadiah Masnindah, Malahubban Fakurazi, Sharida Wong, Sie Chuong Rajaee, Amy Halimah Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages |
| title | Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages |
| title_full | Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages |
| title_fullStr | Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages |
| title_full_unstemmed | Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages |
| title_short | Anti-inflammatory potential of hexane extract of mud lobster (thalassina anomala) in lipopolysaccharide-stimulated raw 264.7 macrophages |
| title_sort | anti inflammatory potential of hexane extract of mud lobster thalassina anomala in lipopolysaccharide stimulated raw 264 7 macrophages |
| topic | crustacean Cell culture Aquaculture |
| work_keys_str_mv | AT zakarianurnadiah antiinflammatorypotentialofhexaneextractofmudlobsterthalassinaanomalainlipopolysaccharidestimulatedraw2647macrophages AT masnindahmalahubban antiinflammatorypotentialofhexaneextractofmudlobsterthalassinaanomalainlipopolysaccharidestimulatedraw2647macrophages AT fakurazisharida antiinflammatorypotentialofhexaneextractofmudlobsterthalassinaanomalainlipopolysaccharidestimulatedraw2647macrophages AT wongsiechuong antiinflammatorypotentialofhexaneextractofmudlobsterthalassinaanomalainlipopolysaccharidestimulatedraw2647macrophages AT rajaeeamyhalimah antiinflammatorypotentialofhexaneextractofmudlobsterthalassinaanomalainlipopolysaccharidestimulatedraw2647macrophages |