Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates
In non-human primates (NHPs), Mycobacterium avium complex (MAC) species are the major source of non-tuberculous mycobacteriosis, causing tuberculous-like lesions in lymph nodes and parenchymatous organs in zoo and wildlife animals. Poor species-specific detection by serological diagnosis has negativ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113552/1/113552.pdf |
| _version_ | 1825940341192130560 |
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| author | Lekko, Yusuf Madaki Che-Amat, Azlan Ooi, Peck Toung Omar, Sharina Ramanoon, Siti Zubaidah Mazlan, Mazlina Abdullah Jesse, Faez Firdaus |
| author_facet | Lekko, Yusuf Madaki Che-Amat, Azlan Ooi, Peck Toung Omar, Sharina Ramanoon, Siti Zubaidah Mazlan, Mazlina Abdullah Jesse, Faez Firdaus |
| author_sort | Lekko, Yusuf Madaki |
| collection | UPM |
| description | In non-human primates (NHPs), Mycobacterium avium complex (MAC) species are the major source of non-tuberculous mycobacteriosis, causing tuberculous-like lesions in lymph nodes and parenchymatous organs in zoo and wildlife animals. Poor species-specific detection by serological diagnosis has negatively impacted the surveillance of MAC on non-human primates. Serum was collected from suspected twelve (n = 12) NHPs with no record of health monitoring, including gibbon (n = 5), capuchins (n = 2), siamang (n = 2), mandrill (n = 1), and orangutan (n = 2). An in-house avian purified protein derivative (aPPD) enzyme-linked immunosorbent assays (ELISA) antibody detection was developed and modified based on the established protocols. The aPPD ELISA for MAC antibodies detection at serum and Protein-G dilutions of 1:200-0.5µg/ml, respectively, detected 3/12 (25%) positive serum. At both serum and Protein-G dilutions of 1:100-0.05 and 1:300-1 µg/ml, the aPPD ELISA detected 12/12 (100%), respectively. The antibody was not detected for an in-house aPPD ELISA with serum and anti-monkey immunoglobulin G (IgG) dilutions at 1:100-0.5 and 1:300-1 µg/ml. However, 2/12 (16%) was detected using serum and anti-monkey IgG dilutions at 1:200-0.05 µg/ml. An in-house aPPD ELISA procedure for MAC antibodies detection in primates, at serum and Protein-G dilutions of 1:100-0.05 and 1:300-1 µg/ml, both have shown sensitivity and specificity of 100%, positive predictive value and negative predictive value of 100%, respectively. The serum and anti-monkey IgG have shown extremely low sensitivity and specificity. In conclusion, the performance of an in-house aPPD ELISA using three different dilutions on serum and conjugates in detecting MAC in a primate has shown that Protein-G horseradish peroxidase, as secondary conjugates were able to detect MAC antibodies. |
| first_indexed | 2024-12-09T02:26:36Z |
| format | Article |
| id | upm.eprints-113552 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-12-09T02:26:36Z |
| publishDate | 2024 |
| publisher | Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-1135522024-11-26T04:15:21Z http://psasir.upm.edu.my/id/eprint/113552/ Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates Lekko, Yusuf Madaki Che-Amat, Azlan Ooi, Peck Toung Omar, Sharina Ramanoon, Siti Zubaidah Mazlan, Mazlina Abdullah Jesse, Faez Firdaus In non-human primates (NHPs), Mycobacterium avium complex (MAC) species are the major source of non-tuberculous mycobacteriosis, causing tuberculous-like lesions in lymph nodes and parenchymatous organs in zoo and wildlife animals. Poor species-specific detection by serological diagnosis has negatively impacted the surveillance of MAC on non-human primates. Serum was collected from suspected twelve (n = 12) NHPs with no record of health monitoring, including gibbon (n = 5), capuchins (n = 2), siamang (n = 2), mandrill (n = 1), and orangutan (n = 2). An in-house avian purified protein derivative (aPPD) enzyme-linked immunosorbent assays (ELISA) antibody detection was developed and modified based on the established protocols. The aPPD ELISA for MAC antibodies detection at serum and Protein-G dilutions of 1:200-0.5µg/ml, respectively, detected 3/12 (25%) positive serum. At both serum and Protein-G dilutions of 1:100-0.05 and 1:300-1 µg/ml, the aPPD ELISA detected 12/12 (100%), respectively. The antibody was not detected for an in-house aPPD ELISA with serum and anti-monkey immunoglobulin G (IgG) dilutions at 1:100-0.5 and 1:300-1 µg/ml. However, 2/12 (16%) was detected using serum and anti-monkey IgG dilutions at 1:200-0.05 µg/ml. An in-house aPPD ELISA procedure for MAC antibodies detection in primates, at serum and Protein-G dilutions of 1:100-0.05 and 1:300-1 µg/ml, both have shown sensitivity and specificity of 100%, positive predictive value and negative predictive value of 100%, respectively. The serum and anti-monkey IgG have shown extremely low sensitivity and specificity. In conclusion, the performance of an in-house aPPD ELISA using three different dilutions on serum and conjugates in detecting MAC in a primate has shown that Protein-G horseradish peroxidase, as secondary conjugates were able to detect MAC antibodies. Universiti Putra Malaysia 2024 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/113552/1/113552.pdf Lekko, Yusuf Madaki and Che-Amat, Azlan and Ooi, Peck Toung and Omar, Sharina and Ramanoon, Siti Zubaidah and Mazlan, Mazlina and Abdullah Jesse, Faez Firdaus (2024) Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates. Pertanika Journal of Tropical Agricultural Science, 47 (2). pp. 549-557. ISSN 1511-3701; eISSN: 2231-8542 http://www.pertanika.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-2911-2023 10.47836/pjtas.47.2.14 |
| spellingShingle | Lekko, Yusuf Madaki Che-Amat, Azlan Ooi, Peck Toung Omar, Sharina Ramanoon, Siti Zubaidah Mazlan, Mazlina Abdullah Jesse, Faez Firdaus Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates |
| title | Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates |
| title_full | Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates |
| title_fullStr | Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates |
| title_full_unstemmed | Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates |
| title_short | Development of an in-house appd elisa for Mycobacterium avium complex (MAC) antibodies detection in zoo primates |
| title_sort | development of an in house appd elisa for mycobacterium avium complex mac antibodies detection in zoo primates |
| url | http://psasir.upm.edu.my/id/eprint/113552/1/113552.pdf |
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