Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species
Application of commercial hormone failed to promote breeding in certain Pangasius species due to the differences of gonad-otropin-releasing hormone specific peptide with species-specific bioactivities. Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide in the reproductive system tha...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Fisheries and Aquatic Sciences
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/113650/1/113650.pdf |
| _version_ | 1825940358937182208 |
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| author | Zamri, Amirah Syafiqah Sahadan, Fatin Nabilah Zulperi, Zarirah Syukri, Fadhil Esa, Yuzine |
| author_facet | Zamri, Amirah Syafiqah Sahadan, Fatin Nabilah Zulperi, Zarirah Syukri, Fadhil Esa, Yuzine |
| author_sort | Zamri, Amirah Syafiqah |
| collection | UPM |
| description | Application of commercial hormone failed to promote breeding in certain Pangasius species due to the differences of gonad-otropin-releasing hormone specific peptide with species-specific bioactivities. Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide in the reproductive system that plays a crucial role in the regulation of reproductive processes. This study was performed to determine and analyse the GnRH genes from commercially important Pangasius sp., Pangasianodon hypophthalmus and Pangasius nasutus. The GnRH1 and GnRH2 genes were amplified and cloned into TOPO vector, followed by phylogenetic analysis of a complete open reading frame (ORF) of GnRH genes. The GnRH1 and GnRH2 genes of P. hypophthal-mus and P. nasutus were detected at 300 bp and 360 bp, encoded for 81 and 87 amino acids, respectively. Amino acid sequence identities revealed high homology of P. hypophthalmus and P. nasutus GnRH1 and GnRH2 genes in comparison with other fish and vertebrates. Phylogenetic tree showed that fish from various families were aggregated into a group of the same order due to their highest identity similarities. It revealed that the vertebrate formed clusters and are grouped according to their GnRH decapeptide and GnRH-associated peptide (GAP) region, indicating a close relationship among GnRH decapeptide and GAP in different vertebrate species. |
| first_indexed | 2024-12-09T02:26:54Z |
| format | Article |
| id | upm.eprints-113650 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-12-09T02:26:54Z |
| publishDate | 2024 |
| publisher | Korean Society of Fisheries and Aquatic Sciences |
| record_format | dspace |
| spelling | upm.eprints-1136502024-11-25T01:23:36Z http://psasir.upm.edu.my/id/eprint/113650/ Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species Zamri, Amirah Syafiqah Sahadan, Fatin Nabilah Zulperi, Zarirah Syukri, Fadhil Esa, Yuzine Application of commercial hormone failed to promote breeding in certain Pangasius species due to the differences of gonad-otropin-releasing hormone specific peptide with species-specific bioactivities. Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide in the reproductive system that plays a crucial role in the regulation of reproductive processes. This study was performed to determine and analyse the GnRH genes from commercially important Pangasius sp., Pangasianodon hypophthalmus and Pangasius nasutus. The GnRH1 and GnRH2 genes were amplified and cloned into TOPO vector, followed by phylogenetic analysis of a complete open reading frame (ORF) of GnRH genes. The GnRH1 and GnRH2 genes of P. hypophthal-mus and P. nasutus were detected at 300 bp and 360 bp, encoded for 81 and 87 amino acids, respectively. Amino acid sequence identities revealed high homology of P. hypophthalmus and P. nasutus GnRH1 and GnRH2 genes in comparison with other fish and vertebrates. Phylogenetic tree showed that fish from various families were aggregated into a group of the same order due to their highest identity similarities. It revealed that the vertebrate formed clusters and are grouped according to their GnRH decapeptide and GnRH-associated peptide (GAP) region, indicating a close relationship among GnRH decapeptide and GAP in different vertebrate species. Korean Society of Fisheries and Aquatic Sciences 2024-06 Article PeerReviewed text en cc_by_nc_4 http://psasir.upm.edu.my/id/eprint/113650/1/113650.pdf Zamri, Amirah Syafiqah and Sahadan, Fatin Nabilah and Zulperi, Zarirah and Syukri, Fadhil and Esa, Yuzine (2024) Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species. Fisheries and Aquatic Sciences, 27 (6). pp. 366-378. ISSN 2234-1749; eISSN: 2234-1757 https://www.e-fas.org/archive/view_article?pid=fas-27-6-366 10.47853/FAS.2024.e36 |
| spellingShingle | Zamri, Amirah Syafiqah Sahadan, Fatin Nabilah Zulperi, Zarirah Syukri, Fadhil Esa, Yuzine Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species |
| title | Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species |
| title_full | Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species |
| title_fullStr | Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species |
| title_full_unstemmed | Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species |
| title_short | Molecular characterization of gonadotropin-releasing hormone (GnRH) genes and their role in reproductive system of Pangasius species |
| title_sort | molecular characterization of gonadotropin releasing hormone gnrh genes and their role in reproductive system of pangasius species |
| url | http://psasir.upm.edu.my/id/eprint/113650/1/113650.pdf |
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