The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo
Blackberry polysaccharides with certain molecular weight distribution have good bioactivity. In this research, type 2 diabetes mice were used to investigate the hypoglycemic effect of blackberry polysaccharides with three different molecular weights, BBP (603.59 kDa), BBP-8 (408.13 kDa) and BBP-24 (...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Royal Society of Chemistry
2024
|
| _version_ | 1824452342419816448 |
|---|---|
| author | Xi, Lai Weibing, Xu Shuyong, Fu Sheng-Hua, Li Xiong, Fu Chin-Ping, Tan Ping-Ping, Wang Zu-Man, Dou Chun, Chen |
| author_facet | Xi, Lai Weibing, Xu Shuyong, Fu Sheng-Hua, Li Xiong, Fu Chin-Ping, Tan Ping-Ping, Wang Zu-Man, Dou Chun, Chen |
| author_sort | Xi, Lai |
| collection | UPM |
| description | Blackberry polysaccharides with certain molecular weight distribution have good bioactivity. In this research, type 2 diabetes mice were used to investigate the hypoglycemic effect of blackberry polysaccharides with three different molecular weights, BBP (603.59 kDa), BBP-8 (408.13 kDa) and BBP-24 (247.62 kDa), through gut microbiota modulation. Blackberry polysaccharides exhibited stronger hypoglycemic activity after degradation, and the FBG of BBP, BBP-8 and BBP-24 was reduced to 20.21 ± 4.17 mmol L−1, 20.6 ± 7.23 mmol L−1 and 17.32 ± 6.59 mmol L−1 and OGTT-AUC was reduced by 14.76%, 19.80% and 25.04%, respectively, after 8-week intervention. Furthermore, 16S rRNA gene sequencing analysis indicated that BBP, BBP-8 and BBP-24 could reshape the diversity and composition of the gut microbiota. From 0 to 4 weeks, the F/B of BBP, BBP-8 and BBP-24 reduced by 56.44%, 47.19% and 62.04%, reaching 3.39, 6.54, and 3.11 in the 8th week, respectively, which suggested the faster utilization of BBP-24. Moreover, the intervention the three blackberry polysaccharides increased the relative abundance of the targeted beneficial bacteria Oscillospira and Bacteroidaceae Bacteroides and decreased the relative abundance of the pathogenic bacterium Allobaculum. In general, the result demonstrated that blackberry polysaccharides with a lower molecular weight are more easily fermented, making the theoretical basis for the development of blackberry polysaccharides as a probiotic food to rapidly regulate intestinal flora for type 2 diabetes. |
| first_indexed | 2025-02-19T02:49:00Z |
| format | Article |
| id | upm.eprints-113837 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-02-19T02:49:00Z |
| publishDate | 2024 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | upm.eprints-1138372025-02-10T07:30:15Z http://psasir.upm.edu.my/id/eprint/113837/ The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo Xi, Lai Weibing, Xu Shuyong, Fu Sheng-Hua, Li Xiong, Fu Chin-Ping, Tan Ping-Ping, Wang Zu-Man, Dou Chun, Chen Blackberry polysaccharides with certain molecular weight distribution have good bioactivity. In this research, type 2 diabetes mice were used to investigate the hypoglycemic effect of blackberry polysaccharides with three different molecular weights, BBP (603.59 kDa), BBP-8 (408.13 kDa) and BBP-24 (247.62 kDa), through gut microbiota modulation. Blackberry polysaccharides exhibited stronger hypoglycemic activity after degradation, and the FBG of BBP, BBP-8 and BBP-24 was reduced to 20.21 ± 4.17 mmol L−1, 20.6 ± 7.23 mmol L−1 and 17.32 ± 6.59 mmol L−1 and OGTT-AUC was reduced by 14.76%, 19.80% and 25.04%, respectively, after 8-week intervention. Furthermore, 16S rRNA gene sequencing analysis indicated that BBP, BBP-8 and BBP-24 could reshape the diversity and composition of the gut microbiota. From 0 to 4 weeks, the F/B of BBP, BBP-8 and BBP-24 reduced by 56.44%, 47.19% and 62.04%, reaching 3.39, 6.54, and 3.11 in the 8th week, respectively, which suggested the faster utilization of BBP-24. Moreover, the intervention the three blackberry polysaccharides increased the relative abundance of the targeted beneficial bacteria Oscillospira and Bacteroidaceae Bacteroides and decreased the relative abundance of the pathogenic bacterium Allobaculum. In general, the result demonstrated that blackberry polysaccharides with a lower molecular weight are more easily fermented, making the theoretical basis for the development of blackberry polysaccharides as a probiotic food to rapidly regulate intestinal flora for type 2 diabetes. Royal Society of Chemistry 2024-07 Article PeerReviewed Xi, Lai and Weibing, Xu and Shuyong, Fu and Sheng-Hua, Li and Xiong, Fu and Chin-Ping, Tan and Ping-Ping, Wang and Zu-Man, Dou and Chun, Chen (2024) The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo. Food and Function, 15 (16). pp. 8586-8603. ISSN 2042-6496; eISSN: 2042-650X https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo01989j 10.1039/d4fo01989j |
| spellingShingle | Xi, Lai Weibing, Xu Shuyong, Fu Sheng-Hua, Li Xiong, Fu Chin-Ping, Tan Ping-Ping, Wang Zu-Man, Dou Chun, Chen The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo |
| title | The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo |
| title_full | The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo |
| title_fullStr | The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo |
| title_full_unstemmed | The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo |
| title_short | The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo |
| title_sort | effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo |
| work_keys_str_mv | AT xilai theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT weibingxu theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT shuyongfu theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT shenghuali theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT xiongfu theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT chinpingtan theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT pingpingwang theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT zumandou theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT chunchen theeffectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT xilai effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT weibingxu effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT shuyongfu effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT shenghuali effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT xiongfu effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT chinpingtan effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT pingpingwang effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT zumandou effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo AT chunchen effectofthemolecularweightofblackberrypolysaccharidesongutmicrobiotamodulationandhypoglycemiceffectinvivo |