Functional analysis of root-preferential oil palm metallothionein promoter in tobacco
Root-specific or preferential promoters are essential to genetically modify plants with beneficial root traits. We have characterised the promoter from an oil palm metallothionein gene (EgMT) and performed a serial 5' deletion analysis to identify the region(s) essential for transgenes expressi...
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| Format: | Article |
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Springer Science and Business Media Deutschland GmbH
2024
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| author | Masura, Subhi Siti Shaharuddin, Noor Azmi Masani, Mat Yunus Abdul Chan, Kuang-Lim Low, Eng-Ti Leslie Chan, Pek-Lan Rahmah, Abdul Rahman Siti Amiruddin, Nadzirah Abdullah, Mohd Puad Azzeme, Azzreena Mohamad Parveez, Ghulam Kadir Ahmad Abd Rasid, Omar |
| author_facet | Masura, Subhi Siti Shaharuddin, Noor Azmi Masani, Mat Yunus Abdul Chan, Kuang-Lim Low, Eng-Ti Leslie Chan, Pek-Lan Rahmah, Abdul Rahman Siti Amiruddin, Nadzirah Abdullah, Mohd Puad Azzeme, Azzreena Mohamad Parveez, Ghulam Kadir Ahmad Abd Rasid, Omar |
| author_sort | Masura, Subhi Siti |
| collection | UPM |
| description | Root-specific or preferential promoters are essential to genetically modify plants with beneficial root traits. We have characterised the promoter from an oil palm metallothionein gene (EgMT) and performed a serial 5' deletion analysis to identify the region(s) essential for transgenes expression in roots. Stable functional characterisation of tobacco transgenic lines using the T1 generation showed that a deletion construct, designated as RSP-2D (1107 bp), directed strong GUS expression at all stages of root development, particularly in mature roots. Other constructs, RSP-2A (2481 bp) and RSP-2C (1639 bp), drove GUS expression in roots with an intensity lower than RSP-2D. The promoter activity was also detectable in seed pods and immature seeds, albeit at lower levels than CaMV35S. The promoter activity may also be induced by wounding as intact GUS staining was observed at the flower- and leaf-cutting sites of T1 samples carrying either RSP-2C or RSP-2D constructs. The promoter sequence contains cis-acting elements that may act as negative regulators and be responsible for root specificity. The results further indicated that the 5' UTR and ATATT sequences are essential for strong promoter activity. This study highlights the potential of RSP-2D promoter as a tool for modifying root traits through genetic engineering. |
| first_indexed | 2025-02-19T02:49:11Z |
| format | Article |
| id | upm.eprints-113899 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-02-19T02:49:11Z |
| publishDate | 2024 |
| publisher | Springer Science and Business Media Deutschland GmbH |
| record_format | dspace |
| spelling | upm.eprints-1138992025-01-16T08:45:55Z http://psasir.upm.edu.my/id/eprint/113899/ Functional analysis of root-preferential oil palm metallothionein promoter in tobacco Masura, Subhi Siti Shaharuddin, Noor Azmi Masani, Mat Yunus Abdul Chan, Kuang-Lim Low, Eng-Ti Leslie Chan, Pek-Lan Rahmah, Abdul Rahman Siti Amiruddin, Nadzirah Abdullah, Mohd Puad Azzeme, Azzreena Mohamad Parveez, Ghulam Kadir Ahmad Abd Rasid, Omar Root-specific or preferential promoters are essential to genetically modify plants with beneficial root traits. We have characterised the promoter from an oil palm metallothionein gene (EgMT) and performed a serial 5' deletion analysis to identify the region(s) essential for transgenes expression in roots. Stable functional characterisation of tobacco transgenic lines using the T1 generation showed that a deletion construct, designated as RSP-2D (1107 bp), directed strong GUS expression at all stages of root development, particularly in mature roots. Other constructs, RSP-2A (2481 bp) and RSP-2C (1639 bp), drove GUS expression in roots with an intensity lower than RSP-2D. The promoter activity was also detectable in seed pods and immature seeds, albeit at lower levels than CaMV35S. The promoter activity may also be induced by wounding as intact GUS staining was observed at the flower- and leaf-cutting sites of T1 samples carrying either RSP-2C or RSP-2D constructs. The promoter sequence contains cis-acting elements that may act as negative regulators and be responsible for root specificity. The results further indicated that the 5' UTR and ATATT sequences are essential for strong promoter activity. This study highlights the potential of RSP-2D promoter as a tool for modifying root traits through genetic engineering. Springer Science and Business Media Deutschland GmbH 2024-10 Article PeerReviewed Masura, Subhi Siti and Shaharuddin, Noor Azmi and Masani, Mat Yunus Abdul and Chan, Kuang-Lim and Low, Eng-Ti Leslie and Chan, Pek-Lan and Rahmah, Abdul Rahman Siti and Amiruddin, Nadzirah and Abdullah, Mohd Puad and Azzeme, Azzreena Mohamad and Parveez, Ghulam Kadir Ahmad and Abd Rasid, Omar (2024) Functional analysis of root-preferential oil palm metallothionein promoter in tobacco. Transgenic Research, 33. pp. 383-397. ISSN 0962-8819; eISSN: 1573-9368 https://link.springer.com/article/10.1007/s11248-024-00396-8?error=cookies_not_supported&code=6a280bfa-4e58-40a2-b9ef-f96e51753270 10.1007/s11248-024-00396-8 |
| spellingShingle | Masura, Subhi Siti Shaharuddin, Noor Azmi Masani, Mat Yunus Abdul Chan, Kuang-Lim Low, Eng-Ti Leslie Chan, Pek-Lan Rahmah, Abdul Rahman Siti Amiruddin, Nadzirah Abdullah, Mohd Puad Azzeme, Azzreena Mohamad Parveez, Ghulam Kadir Ahmad Abd Rasid, Omar Functional analysis of root-preferential oil palm metallothionein promoter in tobacco |
| title | Functional analysis of root-preferential oil palm metallothionein promoter in tobacco |
| title_full | Functional analysis of root-preferential oil palm metallothionein promoter in tobacco |
| title_fullStr | Functional analysis of root-preferential oil palm metallothionein promoter in tobacco |
| title_full_unstemmed | Functional analysis of root-preferential oil palm metallothionein promoter in tobacco |
| title_short | Functional analysis of root-preferential oil palm metallothionein promoter in tobacco |
| title_sort | functional analysis of root preferential oil palm metallothionein promoter in tobacco |
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