Continuous antibody production by phytohemagglutinin-L-aggregated hybridoma cells

Phytohemagglutinin-L (PHA-L) induced aggregation of SP2 myeloma and antibody-producing hybridoma cells. The aggregates were of diverse shapes, and major-axis length of the hybridoma aggregates was mostly 50-80 μm at 1 μg/ml PHA-L and 100-150 μm at 5 μg/ml. PHA-L did not suppress growth rate at these...

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Bibliographic Details
Main Authors: Takamatsu, Hiroyuki, Kawajiri, Hiroo, Takahashi, Yoshitaka, Ali, Abdul Manaf
Format: Article
Language:English
Published: Elsevier 1999
Online Access:http://psasir.upm.edu.my/id/eprint/114072/1/114072.pdf