Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection
Purpose: Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we r...
| Main Authors: | , , , |
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| Format: | Article |
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Springer Science and Business Media Deutschland
2024
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| author | Yahaya, H. Cheah, Y.K. Chee, H.Y. Than, L.T.L. |
| author_facet | Yahaya, H. Cheah, Y.K. Chee, H.Y. Than, L.T.L. |
| author_sort | Yahaya, H. |
| collection | UPM |
| description | Purpose: Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata.
Methodology: The specific LAMP primers for C. glabrata detection were designed and evaluated.
Results: The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species.
Conclusion: The developed molecular method would be a promising tool in the management of invasive candidiasis. |
| first_indexed | 2025-02-19T02:49:27Z |
| format | Article |
| id | upm.eprints-114237 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-02-19T02:49:27Z |
| publishDate | 2024 |
| publisher | Springer Science and Business Media Deutschland |
| record_format | dspace |
| spelling | upm.eprints-1142372025-01-08T04:44:03Z http://psasir.upm.edu.my/id/eprint/114237/ Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection Yahaya, H. Cheah, Y.K. Chee, H.Y. Than, L.T.L. Purpose: Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata. Methodology: The specific LAMP primers for C. glabrata detection were designed and evaluated. Results: The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species. Conclusion: The developed molecular method would be a promising tool in the management of invasive candidiasis. Springer Science and Business Media Deutschland 2024-09-23 Article PeerReviewed Yahaya, H. and Cheah, Y.K. and Chee, H.Y. and Than, L.T.L. (2024) Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection. European Journal of Clinical Microbiology and Infectious Diseases, 43. pp. 2211-2214. ISSN 0934-9723; eISSN: 1435-4373 https://link.springer.com/article/10.1007/s10096-024-04934-4?error=cookies_not_supported&code=4082e6b1-a021-4412-9a8d-95655f03156c 10.1007/s10096-024-04934-4 |
| spellingShingle | Yahaya, H. Cheah, Y.K. Chee, H.Y. Than, L.T.L. Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection |
| title | Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection |
| title_full | Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection |
| title_fullStr | Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection |
| title_full_unstemmed | Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection |
| title_short | Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection |
| title_sort | development and evaluation of a loop mediated isothermal amplification lamp method for candida glabrata detection |
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