Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7
Extensive studies on γ-aminobutyric acid (GABA) over decades highlight its significant physiological and pharmacological effects on humans. GABA produced using microbe is favoured compared to enzymatic and chemical methods due to operational ease and reduced harmful pollutant formation. This study f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114266/1/114266.pdf |
| _version_ | 1824452376565645312 |
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| author | Pannerchelvan, Sangkaran Faizal Wong, Fadzlie Wong Wasoh, Helmi Mohamed, Mohd Shamzi Mohamad, Rosfarizan Halim, Murni |
| author_facet | Pannerchelvan, Sangkaran Faizal Wong, Fadzlie Wong Wasoh, Helmi Mohamed, Mohd Shamzi Mohamad, Rosfarizan Halim, Murni |
| author_sort | Pannerchelvan, Sangkaran |
| collection | UPM |
| description | Extensive studies on γ-aminobutyric acid (GABA) over decades highlight its significant physiological and pharmacological effects on humans. GABA produced using microbe is favoured compared to enzymatic and chemical methods due to operational ease and reduced harmful pollutant formation. This study focused on increasing γ-aminobutyric acid (GABA) biosynthesis from Lactiplantibacillus plantarum B7, employing a multi-step optimisation strategy. An unoptimised cultivation approach yielded a maximum GABA of 11.68 ± 0.04 g/L and viable cell count of 10.47 ± 0.01 log CFU/mL at 48 h. A nutrient-rich medium was developed through single-parameter optimisation, comprising 1%, 2.5% and 0.0002% of glucose, yeast extract and each trace element (CaCO3, KI, and Tween 80) respectively. Temperature, pH, incubation period, initial concentration of monosodium glutamate (MSG) and pyridoxal-5’-phosphate (PLP) demonstrated significant contributions towards GABA production and cell growth as determined using a two-level factorial design. Steepest ascent identified optimal conditions (36°C, pH 5.5, 370 mM MSG, and 0.7 mM PLP), resulting in 30.50 g/L GABA and 11.51 log CFU/mL at 60 h. Further refinement via a central composite experiment yielded optimal conditions (temperature-35.6°C, pH-5.66, initial MSG concentration-335.61 mM, PLP concentration-0.723 mM) with improved GABA production (32.18 g/L) and cell growth (11.52 log CFU/mL) over 63.66 h. Therefore, this approach utilising lactic acid bacteria capable of GABA synthesis holds promise for mass-produced, enhanced-functional foods. |
| first_indexed | 2025-02-19T02:49:33Z |
| format | Article |
| id | upm.eprints-114266 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2025-02-19T02:49:33Z |
| publishDate | 2024 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | dspace |
| spelling | upm.eprints-1142662025-01-13T02:58:11Z http://psasir.upm.edu.my/id/eprint/114266/ Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7 Pannerchelvan, Sangkaran Faizal Wong, Fadzlie Wong Wasoh, Helmi Mohamed, Mohd Shamzi Mohamad, Rosfarizan Halim, Murni Extensive studies on γ-aminobutyric acid (GABA) over decades highlight its significant physiological and pharmacological effects on humans. GABA produced using microbe is favoured compared to enzymatic and chemical methods due to operational ease and reduced harmful pollutant formation. This study focused on increasing γ-aminobutyric acid (GABA) biosynthesis from Lactiplantibacillus plantarum B7, employing a multi-step optimisation strategy. An unoptimised cultivation approach yielded a maximum GABA of 11.68 ± 0.04 g/L and viable cell count of 10.47 ± 0.01 log CFU/mL at 48 h. A nutrient-rich medium was developed through single-parameter optimisation, comprising 1%, 2.5% and 0.0002% of glucose, yeast extract and each trace element (CaCO3, KI, and Tween 80) respectively. Temperature, pH, incubation period, initial concentration of monosodium glutamate (MSG) and pyridoxal-5’-phosphate (PLP) demonstrated significant contributions towards GABA production and cell growth as determined using a two-level factorial design. Steepest ascent identified optimal conditions (36°C, pH 5.5, 370 mM MSG, and 0.7 mM PLP), resulting in 30.50 g/L GABA and 11.51 log CFU/mL at 60 h. Further refinement via a central composite experiment yielded optimal conditions (temperature-35.6°C, pH-5.66, initial MSG concentration-335.61 mM, PLP concentration-0.723 mM) with improved GABA production (32.18 g/L) and cell growth (11.52 log CFU/mL) over 63.66 h. Therefore, this approach utilising lactic acid bacteria capable of GABA synthesis holds promise for mass-produced, enhanced-functional foods. Journal of Pure and Applied Microbiology 2024-08-29 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/114266/1/114266.pdf Pannerchelvan, Sangkaran and Faizal Wong, Fadzlie Wong and Wasoh, Helmi and Mohamed, Mohd Shamzi and Mohamad, Rosfarizan and Halim, Murni (2024) Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7. Journal of Pure and Applied Microbiology, 18 (3). art. no. 9495. pp. 1931-1948. ISSN 0973-7510; eISSN: 2581-690X https://microbiologyjournal.org/medium-formulation-and-optimisation-of-fermentation-condition-enhancing-%ce%b3-aminobutyric-acid-gaba-biosynthesis-by-lactiplantibacillus-plantarum-b7/ 10.22207/JPAM.18.3.44 |
| spellingShingle | Pannerchelvan, Sangkaran Faizal Wong, Fadzlie Wong Wasoh, Helmi Mohamed, Mohd Shamzi Mohamad, Rosfarizan Halim, Murni Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7 |
| title | Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7 |
| title_full | Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7 |
| title_fullStr | Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7 |
| title_full_unstemmed | Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7 |
| title_short | Medium formulation and optimisation of fermentation condition enhancing g-aminobutyric Acid (GABA) biosynthesis by Lactiplantibacillus plantarum B7 |
| title_sort | medium formulation and optimisation of fermentation condition enhancing g aminobutyric acid gaba biosynthesis by lactiplantibacillus plantarum b7 |
| url | http://psasir.upm.edu.my/id/eprint/114266/1/114266.pdf |
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