Polyphenoloxidase from guava (Psidium guajava L.)
Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. Polyacrylamide gel electrophoresis indicated t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Published: |
John Wiley & Sons
1985
|
| _version_ | 1824452388660969472 |
|---|---|
| author | Augustin, M.A. Ghazali, Hasanah M. Hashim, Hasimah |
| author_facet | Augustin, M.A. Ghazali, Hasanah M. Hashim, Hasimah |
| author_sort | Augustin, M.A. |
| collection | UPM |
| description | Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. Polyacrylamide gel electrophoresis indicated that PPO had a molecular weight in the region of 120000–140000. The pH optimum of the enzyme was found to be 7.2. Heat inactivation studies showed that heating for 1.1, 3.5, and 10.1 min at 75, 65 and 55°C respectively caused a 50% loss in enzymic activity. The enzyme‐catalysed browning reaction was significantly inhibited in the presence of sodium metabisulphite, L‐cysteine hydrochloride and ascorbic acid. |
| first_indexed | 2025-02-19T02:49:44Z |
| format | Article |
| id | upm.eprints-114332 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-02-19T02:49:44Z |
| publishDate | 1985 |
| publisher | John Wiley & Sons |
| record_format | dspace |
| spelling | upm.eprints-1143322025-01-14T02:57:23Z http://psasir.upm.edu.my/id/eprint/114332/ Polyphenoloxidase from guava (Psidium guajava L.) Augustin, M.A. Ghazali, Hasanah M. Hashim, Hasimah Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. Polyacrylamide gel electrophoresis indicated that PPO had a molecular weight in the region of 120000–140000. The pH optimum of the enzyme was found to be 7.2. Heat inactivation studies showed that heating for 1.1, 3.5, and 10.1 min at 75, 65 and 55°C respectively caused a 50% loss in enzymic activity. The enzyme‐catalysed browning reaction was significantly inhibited in the presence of sodium metabisulphite, L‐cysteine hydrochloride and ascorbic acid. John Wiley & Sons 1985 Article PeerReviewed Augustin, M.A. and Ghazali, Hasanah M. and Hashim, Hasimah (1985) Polyphenoloxidase from guava (Psidium guajava L.). Journal of the Science of Food and Agriculture, 36 (12). pp. 1259-1265. ISSN 0022-5142; eISSN: 1097-0010 https://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740361209 10.1002/jsfa.2740361209 |
| spellingShingle | Augustin, M.A. Ghazali, Hasanah M. Hashim, Hasimah Polyphenoloxidase from guava (Psidium guajava L.) |
| title | Polyphenoloxidase from guava (Psidium guajava L.) |
| title_full | Polyphenoloxidase from guava (Psidium guajava L.) |
| title_fullStr | Polyphenoloxidase from guava (Psidium guajava L.) |
| title_full_unstemmed | Polyphenoloxidase from guava (Psidium guajava L.) |
| title_short | Polyphenoloxidase from guava (Psidium guajava L.) |
| title_sort | polyphenoloxidase from guava psidium guajava l |
| work_keys_str_mv | AT augustinma polyphenoloxidasefromguavapsidiumguajaval AT ghazalihasanahm polyphenoloxidasefromguavapsidiumguajaval AT hashimhasimah polyphenoloxidasefromguavapsidiumguajaval |