Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers
The effects of different concentrations of BAP and TDZ on somaclonal variation based on genetic and phenotypic variability among micropropagated shoot tips of banana cultivars; ‘Berangan Intan’, ‘Berangan’ and ‘Rastali’ were studied. With the highest concentrations of both BAP and TDZ up to 44.4µM a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Canadian Center of Science and Education
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/11434/1/Study%20of%20Genetic%20and%20Phenotypic%20Variability%20among%20Somaclones%20Induced%20by%20BAP%20and%20TDZ%20in%20Micropropagated%20Shoot%20Tips%20of%20Banana.pdf |
| _version_ | 1825944908752486400 |
|---|---|
| author | Bidabadi, Siamak Shirani Meon, Sariah Wahab, Zakaria Mahmood, Maziah |
| author_facet | Bidabadi, Siamak Shirani Meon, Sariah Wahab, Zakaria Mahmood, Maziah |
| author_sort | Bidabadi, Siamak Shirani |
| collection | UPM |
| description | The effects of different concentrations of BAP and TDZ on somaclonal variation based on genetic and phenotypic variability among micropropagated shoot tips of banana cultivars; ‘Berangan Intan’, ‘Berangan’ and ‘Rastali’ were studied. With the highest concentrations of both BAP and TDZ up to 44.4µM and 7.5 µM, respectively, most shoots were curled and other morphological changes such as hyperhydricity and undifferentiated shoots were observed. Detectable genetic changes to reveal stable variations among 27 micropropagated variants produced by BAP and TDZ were confirmed using RAPD markers. The percentage of polymorphism among somaclones of cultivars; Berangan Intan, Berangan and Rastali regenerated by TDZ was 24.4, 27.3 and 29.5, respectively which was higher than BAP with the percentages of 16.7, 24.2 and 24.7, respectively. The number of scorable bands among the RAPD profiles obtained with 19 primers tested in this study varied from 6 for opa11 and opk03 to 18 by opc01 in somaclones derived from BAP and 4 for opk03 to 14 with opa04, opb18 and opc04 in the case of TDZ. Cluster analysis of the somaclones regenerated with each BAP and TDZ resulted in the formation of two major groups, one belonging to Berangan Intan and Berangan (AAA) and the other to Rastali (AAB). From each dendrogram caused by BAP and TDZ, it could be concluded that regenerated somaclones by higher concentrations of both BAP and TDZ accumulated much genetic variation compared to control and lower concentrations of each BAP and TDZ. |
| first_indexed | 2024-03-06T07:23:16Z |
| format | Article |
| id | upm.eprints-11434 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:23:16Z |
| publishDate | 2010 |
| publisher | Canadian Center of Science and Education |
| record_format | dspace |
| spelling | upm.eprints-114342015-09-02T01:10:20Z http://psasir.upm.edu.my/id/eprint/11434/ Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers Bidabadi, Siamak Shirani Meon, Sariah Wahab, Zakaria Mahmood, Maziah The effects of different concentrations of BAP and TDZ on somaclonal variation based on genetic and phenotypic variability among micropropagated shoot tips of banana cultivars; ‘Berangan Intan’, ‘Berangan’ and ‘Rastali’ were studied. With the highest concentrations of both BAP and TDZ up to 44.4µM and 7.5 µM, respectively, most shoots were curled and other morphological changes such as hyperhydricity and undifferentiated shoots were observed. Detectable genetic changes to reveal stable variations among 27 micropropagated variants produced by BAP and TDZ were confirmed using RAPD markers. The percentage of polymorphism among somaclones of cultivars; Berangan Intan, Berangan and Rastali regenerated by TDZ was 24.4, 27.3 and 29.5, respectively which was higher than BAP with the percentages of 16.7, 24.2 and 24.7, respectively. The number of scorable bands among the RAPD profiles obtained with 19 primers tested in this study varied from 6 for opa11 and opk03 to 18 by opc01 in somaclones derived from BAP and 4 for opk03 to 14 with opa04, opb18 and opc04 in the case of TDZ. Cluster analysis of the somaclones regenerated with each BAP and TDZ resulted in the formation of two major groups, one belonging to Berangan Intan and Berangan (AAA) and the other to Rastali (AAB). From each dendrogram caused by BAP and TDZ, it could be concluded that regenerated somaclones by higher concentrations of both BAP and TDZ accumulated much genetic variation compared to control and lower concentrations of each BAP and TDZ. Canadian Center of Science and Education 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11434/1/Study%20of%20Genetic%20and%20Phenotypic%20Variability%20among%20Somaclones%20Induced%20by%20BAP%20and%20TDZ%20in%20Micropropagated%20Shoot%20Tips%20of%20Banana.pdf Bidabadi, Siamak Shirani and Meon, Sariah and Wahab, Zakaria and Mahmood, Maziah (2010) Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers. Journal of Agricultural Science, 2 (3). pp. 49-60. ISSN 1916-9752 http://www.ccsenet.org/journal/index.php/jas/article/view/5556 English |
| spellingShingle | Bidabadi, Siamak Shirani Meon, Sariah Wahab, Zakaria Mahmood, Maziah Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers |
| title | Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers |
| title_full | Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers |
| title_fullStr | Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers |
| title_full_unstemmed | Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers |
| title_short | Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers |
| title_sort | study of genetic and phenotypic variability among somaclones induced by bap and tdz in micropropagated shoot tips of banana musa spp using rapd markers |
| url | http://psasir.upm.edu.my/id/eprint/11434/1/Study%20of%20Genetic%20and%20Phenotypic%20Variability%20among%20Somaclones%20Induced%20by%20BAP%20and%20TDZ%20in%20Micropropagated%20Shoot%20Tips%20of%20Banana.pdf |
| work_keys_str_mv | AT bidabadisiamakshirani studyofgeneticandphenotypicvariabilityamongsomaclonesinducedbybapandtdzinmicropropagatedshoottipsofbananamusasppusingrapdmarkers AT meonsariah studyofgeneticandphenotypicvariabilityamongsomaclonesinducedbybapandtdzinmicropropagatedshoottipsofbananamusasppusingrapdmarkers AT wahabzakaria studyofgeneticandphenotypicvariabilityamongsomaclonesinducedbybapandtdzinmicropropagatedshoottipsofbananamusasppusingrapdmarkers AT mahmoodmaziah studyofgeneticandphenotypicvariabilityamongsomaclonesinducedbybapandtdzinmicropropagatedshoottipsofbananamusasppusingrapdmarkers |