Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans

Incubation of unfermented and partly fermented cocoa beans in acetate buffer, pH 5.5, at 45 °C increased yellowness, total colour differences and fermentation index value of the cocoa bean powders and decreased cocoa procyanidins (monomers to pentamers), and their astringency. Fermentation index and...

Full description

Bibliographic Details
Main Authors: Misnawi, Jinap, S., Jamilah, B., Nazamid, S.
Format: Article
Language:English
Published: Blackwell Publishing 2003
Online Access:http://psasir.upm.edu.my/id/eprint/114444/1/114444.pdf
_version_ 1824452409234030592
author Misnawi
Jinap, S.
Jamilah, B.
Nazamid, S.
author_facet Misnawi
Jinap, S.
Jamilah, B.
Nazamid, S.
author_sort Misnawi
collection UPM
description Incubation of unfermented and partly fermented cocoa beans in acetate buffer, pH 5.5, at 45 °C increased yellowness, total colour differences and fermentation index value of the cocoa bean powders and decreased cocoa procyanidins (monomers to pentamers), and their astringency. Fermentation index and (-)-epicatechin content, equivalent to those of fully fermented beans, were reached by unfermented beans after 4-8-h incubation, but not by partly fermented beans even after 16 h. During incubation of partly fermented cocoa beans enriched with polyphenol oxidase, yellowness and fermentation index value were increased, whilst (-)-epicatechin was decreased. Tyrosinase had a less significant effect in yellow colour formation, but showed a significant reduction of (-)-epicatechin and increase in fermentation index compared with crude cocoa polyphenol oxidase. However, both enzymes have similar effects on procyanidin degradation and astringent taste reduction. Incubation of cocoa beans for 16 h increased the cut test score of unfermented and partly fermented beans by 50 and 30%, respectively.
first_indexed 2025-02-19T02:50:04Z
format Article
id upm.eprints-114444
institution Universiti Putra Malaysia
language English
last_indexed 2025-02-19T02:50:04Z
publishDate 2003
publisher Blackwell Publishing
record_format dspace
spelling upm.eprints-1144442025-02-05T03:53:04Z http://psasir.upm.edu.my/id/eprint/114444/ Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans Misnawi Jinap, S. Jamilah, B. Nazamid, S. Incubation of unfermented and partly fermented cocoa beans in acetate buffer, pH 5.5, at 45 °C increased yellowness, total colour differences and fermentation index value of the cocoa bean powders and decreased cocoa procyanidins (monomers to pentamers), and their astringency. Fermentation index and (-)-epicatechin content, equivalent to those of fully fermented beans, were reached by unfermented beans after 4-8-h incubation, but not by partly fermented beans even after 16 h. During incubation of partly fermented cocoa beans enriched with polyphenol oxidase, yellowness and fermentation index value were increased, whilst (-)-epicatechin was decreased. Tyrosinase had a less significant effect in yellow colour formation, but showed a significant reduction of (-)-epicatechin and increase in fermentation index compared with crude cocoa polyphenol oxidase. However, both enzymes have similar effects on procyanidin degradation and astringent taste reduction. Incubation of cocoa beans for 16 h increased the cut test score of unfermented and partly fermented beans by 50 and 30%, respectively. Blackwell Publishing 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114444/1/114444.pdf Misnawi and Jinap, S. and Jamilah, B. and Nazamid, S. (2003) Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans. International Journal of Food Science and Technology, 38 (3). pp. 285-295. ISSN 0950-5423; eISSN: 0950-5423 https://ifst.onlinelibrary.wiley.com/doi/10.1046/j.1365-2621.2003.00674.x 10.1046/j.1365-2621.2003.00674.x
spellingShingle Misnawi
Jinap, S.
Jamilah, B.
Nazamid, S.
Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans
title Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans
title_full Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans
title_fullStr Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans
title_full_unstemmed Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans
title_short Effects of incubation and polyphenol oxidase enrichment on colour, fermentation index, procyanidins and astringency of unfermented and partly fermented cocoa beans
title_sort effects of incubation and polyphenol oxidase enrichment on colour fermentation index procyanidins and astringency of unfermented and partly fermented cocoa beans
url http://psasir.upm.edu.my/id/eprint/114444/1/114444.pdf
work_keys_str_mv AT misnawi effectsofincubationandpolyphenoloxidaseenrichmentoncolourfermentationindexprocyanidinsandastringencyofunfermentedandpartlyfermentedcocoabeans
AT jinaps effectsofincubationandpolyphenoloxidaseenrichmentoncolourfermentationindexprocyanidinsandastringencyofunfermentedandpartlyfermentedcocoabeans
AT jamilahb effectsofincubationandpolyphenoloxidaseenrichmentoncolourfermentationindexprocyanidinsandastringencyofunfermentedandpartlyfermentedcocoabeans
AT nazamids effectsofincubationandpolyphenoloxidaseenrichmentoncolourfermentationindexprocyanidinsandastringencyofunfermentedandpartlyfermentedcocoabeans