GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma
The aim of this study is to investigate the potential correlation between the expression of phosphorylated glycogen synthase kinase-3β (phospho-GSK-3β) and β-catenin, and the mutations of β-catenin gene at the consensus GSK-3β phosphorylation site. The reason for this approach is to gain a better un...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier Ireland
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114566/1/114566.pdf |
| _version_ | 1825940513932443648 |
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| author | Ban, Ke Chen Singh, Harjit Krishnan, R. Seow, Heng Fong |
| author_facet | Ban, Ke Chen Singh, Harjit Krishnan, R. Seow, Heng Fong |
| author_sort | Ban, Ke Chen |
| collection | UPM |
| description | The aim of this study is to investigate the potential correlation between the expression of phosphorylated glycogen synthase kinase-3β (phospho-GSK-3β) and β-catenin, and the mutations of β-catenin gene at the consensus GSK-3β phosphorylation site. The reason for this approach is to gain a better understanding of the molecular mechanisms of hepatocarcinogenesis in Malaysia. The expression of phospho-GSK-3β and β-catenin by immunohistochemistry and the mutations of β-catenin were studied in 23 hepatocellular carcinoma (HCC) and surrounding tissues. Overexpression of phospho-GSK-3β and β-catenin was found in 12/23 (52.2%) and 13/23 (56.5%) in HCC tissues, 6/23 (26.1%) and 9/23 (39.1%) in surrounding tissues, respectively. Overexpression of β-catenin in HCC tissues compared to the surrounding liver tissue was found to be higher in HCC tissues (p=0.015). In addition, we found that the expression of phospho-GSK-3β was related with the accumulation of β-catenin in surrounding tissues (p<0.05). The expression of phospho-GSK-3β and its association with the development of HCC is reported for the first time. In addition, this is the first report from Malaysia which shows that there are no mutations at the GSK-3β consensus phosphorylation sites on β-catenin gene in all 23 paired HCC and surrounding tissues. This result differed from HCC in geographical areas with high aflatoxin exposure. |
| first_indexed | 2025-03-07T13:02:51Z |
| format | Article |
| id | upm.eprints-114566 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2025-03-07T13:02:51Z |
| publishDate | 2003 |
| publisher | Elsevier Ireland |
| record_format | dspace |
| spelling | upm.eprints-1145662025-03-04T08:03:03Z http://psasir.upm.edu.my/id/eprint/114566/ GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma Ban, Ke Chen Singh, Harjit Krishnan, R. Seow, Heng Fong The aim of this study is to investigate the potential correlation between the expression of phosphorylated glycogen synthase kinase-3β (phospho-GSK-3β) and β-catenin, and the mutations of β-catenin gene at the consensus GSK-3β phosphorylation site. The reason for this approach is to gain a better understanding of the molecular mechanisms of hepatocarcinogenesis in Malaysia. The expression of phospho-GSK-3β and β-catenin by immunohistochemistry and the mutations of β-catenin were studied in 23 hepatocellular carcinoma (HCC) and surrounding tissues. Overexpression of phospho-GSK-3β and β-catenin was found in 12/23 (52.2%) and 13/23 (56.5%) in HCC tissues, 6/23 (26.1%) and 9/23 (39.1%) in surrounding tissues, respectively. Overexpression of β-catenin in HCC tissues compared to the surrounding liver tissue was found to be higher in HCC tissues (p=0.015). In addition, we found that the expression of phospho-GSK-3β was related with the accumulation of β-catenin in surrounding tissues (p<0.05). The expression of phospho-GSK-3β and its association with the development of HCC is reported for the first time. In addition, this is the first report from Malaysia which shows that there are no mutations at the GSK-3β consensus phosphorylation sites on β-catenin gene in all 23 paired HCC and surrounding tissues. This result differed from HCC in geographical areas with high aflatoxin exposure. Elsevier Ireland 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114566/1/114566.pdf Ban, Ke Chen and Singh, Harjit and Krishnan, R. and Seow, Heng Fong (2003) GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma. Cancer Letters, 199 (2). pp. 201-208. ISSN 0304-3835; eISSN: 0304-3835 https://linkinghub.elsevier.com/retrieve/pii/S030438350300421X 10.1016/S0304-3835(03)00421-X |
| spellingShingle | Ban, Ke Chen Singh, Harjit Krishnan, R. Seow, Heng Fong GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma |
| title | GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma |
| title_full | GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma |
| title_fullStr | GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma |
| title_full_unstemmed | GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma |
| title_short | GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma |
| title_sort | gsk 3β phosphorylation and alteration of β catenin in hepatocellular carcinoma |
| url | http://psasir.upm.edu.my/id/eprint/114566/1/114566.pdf |
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