Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans
Particulate fractions of Thiobacillus denitrificans catalyse the phosphorylation of ADP to ATP during the oxidation of various inorganic sulphur compounds or NADH via an electron transport chain. On the other hand, a "soluble" cell-free fraction synthesized ATP from APS and inorganic phosp...
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| Format: | Article |
| Language: | English |
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Springer-Verlag
1980
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114777/1/114777.pdf |
| _version_ | 1824452468218527744 |
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| author | Aminuddin, M. |
| author_facet | Aminuddin, M. |
| author_sort | Aminuddin, M. |
| collection | UPM |
| description | Particulate fractions of Thiobacillus denitrificans catalyse the phosphorylation of ADP to ATP during the oxidation of various inorganic sulphur compounds or NADH via an electron transport chain. On the other hand, a "soluble" cell-free fraction synthesized ATP from APS and inorganic phosphate. The production of ATP was verified either by the firefly luciferin-luciferase enzyme system or by the incorporation of 32Pi into ATP. During the oxidation of sulphide, sulphite and NADH the production of ATP from ADP by particulate fractions is inhibited by compounds that inhibit electron transfer and by uncouplers of oxidative phosphorylation. However, these compounds had little effect on the production of ATP from AMP during the oxidation of sulphite by the soluble fraction. NADH was the most effective electron donor for oxidative phosphorylation. The soluble fraction contained high activities of ATP sulphurylase, inorganic pyrophosphatase and adenylate kinase but ADP sulphurylase activity was relatively low. The effects of inhibitors on ATP production from APS and Pi are compared with those on adenylate kinase and ATP sulphurylase. © 1980 Springer-Verlag. |
| first_indexed | 2025-02-19T02:51:00Z |
| format | Article |
| id | upm.eprints-114777 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2025-02-19T02:51:00Z |
| publishDate | 1980 |
| publisher | Springer-Verlag |
| record_format | dspace |
| spelling | upm.eprints-1147772025-01-31T02:35:54Z http://psasir.upm.edu.my/id/eprint/114777/ Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans Aminuddin, M. Particulate fractions of Thiobacillus denitrificans catalyse the phosphorylation of ADP to ATP during the oxidation of various inorganic sulphur compounds or NADH via an electron transport chain. On the other hand, a "soluble" cell-free fraction synthesized ATP from APS and inorganic phosphate. The production of ATP was verified either by the firefly luciferin-luciferase enzyme system or by the incorporation of 32Pi into ATP. During the oxidation of sulphide, sulphite and NADH the production of ATP from ADP by particulate fractions is inhibited by compounds that inhibit electron transfer and by uncouplers of oxidative phosphorylation. However, these compounds had little effect on the production of ATP from AMP during the oxidation of sulphite by the soluble fraction. NADH was the most effective electron donor for oxidative phosphorylation. The soluble fraction contained high activities of ATP sulphurylase, inorganic pyrophosphatase and adenylate kinase but ADP sulphurylase activity was relatively low. The effects of inhibitors on ATP production from APS and Pi are compared with those on adenylate kinase and ATP sulphurylase. © 1980 Springer-Verlag. Springer-Verlag 1980 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114777/1/114777.pdf Aminuddin, M. (1980) Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans. Archives of Microbiology, 128 (1). pp. 19-25. ISSN 1432-072X; eISSN: 0302-8933 https://link.springer.com/article/10.1007/BF00422300?error=cookies_not_supported&code=e141395f-0da4-4715-9caa-aaa95ab66743 10.1007/BF00422300 |
| spellingShingle | Aminuddin, M. Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans |
| title | Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans |
| title_full | Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans |
| title_fullStr | Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans |
| title_full_unstemmed | Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans |
| title_short | Substrate level versus oxidative phosphorylation in the generation of ATP in Thiobacillus denitrificans |
| title_sort | substrate level versus oxidative phosphorylation in the generation of atp in thiobacillus denitrificans |
| url | http://psasir.upm.edu.my/id/eprint/114777/1/114777.pdf |
| work_keys_str_mv | AT aminuddinm substratelevelversusoxidativephosphorylationinthegenerationofatpinthiobacillusdenitrificans |