Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight
The Central Composite Design (CCD) within the Response Surface Methodology (RSM) was applied to optimize the enzymatic hydrolysis process. This process used Alcalase® to hydrolyze Bactronophorus thoracites protein with the goal of maximizing its antimicrobial effects. Four distinct parameters were i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Science, Universiti Malaya
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/114783/1/114783.pdf |
| _version_ | 1824452469477867520 |
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| author | Jamal, Siti Norazura Lamasudin, Dhilia Udie Muhialdin, Belal J. Saidi, Noor Baity Song, Lai Kok Yusof, Mohd Termizi |
| author_facet | Jamal, Siti Norazura Lamasudin, Dhilia Udie Muhialdin, Belal J. Saidi, Noor Baity Song, Lai Kok Yusof, Mohd Termizi |
| author_sort | Jamal, Siti Norazura |
| collection | UPM |
| description | The Central Composite Design (CCD) within the Response Surface Methodology (RSM) was applied to optimize the enzymatic hydrolysis process. This process used Alcalase® to hydrolyze Bactronophorus thoracites protein with the goal of maximizing its antimicrobial effects. Four distinct parameters were identified as independent variables: pH (A: 8.5–10.5), temperature (B: 45–65 °C), hydrolysis time (C: 120–360 min), and enzyme-to-substrate ratio (D: 1.45%–2.65% w/v). Meanwhile, the antimicrobial activity was chosen as the response variable, specifically against Pantoea ananatis (Y1) and Pantoea stewartii (Y2). According to the findings, the constructed quadratic polynomial model showed a significant correlation with the experimental data, as evidenced by the coefficient of determination (R2) values for antimicrobial activity: Y1 being 0.9893 (p < 0.0001) and Y2 at 0.9848 (p < 0.0001). Optimal antimicrobial activity for Bactronophorus thoracites protein hydrolysates (BTPH) was recorded at 46.748% against P. ananatis and 40.768% against P. stewartii. This result was observed under the optimal conditions of pH 9.5, temperature 55ºC, hydrolysis duration of 240 minutes, and 2.05% w/v enzyme-to-substrate ratio. There was a notable alignment between the actual and predicted values from our models, with the Residual Standard Error (RSE) values falling under 5%. Furthermore, the established Minimum Inhibitory Concentration (MIC) was 250µg/mL, and the Minimum Bactericidal Concentration (MBC) was 500µg/mL for both P. ananatis and P. stewartii. In conclusion, the findings suggest that the refined BTPH has great promise as an effective bioactive component for agricultural use. |
| first_indexed | 2025-02-19T02:51:01Z |
| format | Article |
| id | upm.eprints-114783 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2025-02-19T02:51:01Z |
| publishDate | 2024 |
| publisher | Faculty of Science, Universiti Malaya |
| record_format | dspace |
| spelling | upm.eprints-1147832025-01-31T03:10:25Z http://psasir.upm.edu.my/id/eprint/114783/ Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight Jamal, Siti Norazura Lamasudin, Dhilia Udie Muhialdin, Belal J. Saidi, Noor Baity Song, Lai Kok Yusof, Mohd Termizi The Central Composite Design (CCD) within the Response Surface Methodology (RSM) was applied to optimize the enzymatic hydrolysis process. This process used Alcalase® to hydrolyze Bactronophorus thoracites protein with the goal of maximizing its antimicrobial effects. Four distinct parameters were identified as independent variables: pH (A: 8.5–10.5), temperature (B: 45–65 °C), hydrolysis time (C: 120–360 min), and enzyme-to-substrate ratio (D: 1.45%–2.65% w/v). Meanwhile, the antimicrobial activity was chosen as the response variable, specifically against Pantoea ananatis (Y1) and Pantoea stewartii (Y2). According to the findings, the constructed quadratic polynomial model showed a significant correlation with the experimental data, as evidenced by the coefficient of determination (R2) values for antimicrobial activity: Y1 being 0.9893 (p < 0.0001) and Y2 at 0.9848 (p < 0.0001). Optimal antimicrobial activity for Bactronophorus thoracites protein hydrolysates (BTPH) was recorded at 46.748% against P. ananatis and 40.768% against P. stewartii. This result was observed under the optimal conditions of pH 9.5, temperature 55ºC, hydrolysis duration of 240 minutes, and 2.05% w/v enzyme-to-substrate ratio. There was a notable alignment between the actual and predicted values from our models, with the Residual Standard Error (RSE) values falling under 5%. Furthermore, the established Minimum Inhibitory Concentration (MIC) was 250µg/mL, and the Minimum Bactericidal Concentration (MBC) was 500µg/mL for both P. ananatis and P. stewartii. In conclusion, the findings suggest that the refined BTPH has great promise as an effective bioactive component for agricultural use. Faculty of Science, Universiti Malaya 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114783/1/114783.pdf Jamal, Siti Norazura and Lamasudin, Dhilia Udie and Muhialdin, Belal J. and Saidi, Noor Baity and Song, Lai Kok and Yusof, Mohd Termizi (2024) Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight. Malaysian Journal of Science, 43 (3). pp. 1-12. ISSN 1394-3065; eISSN: 2600-8688 https://mjs.um.edu.my/index.php/MJS/article/view/44631 10.22452/mjs.vol43no3.1 |
| spellingShingle | Jamal, Siti Norazura Lamasudin, Dhilia Udie Muhialdin, Belal J. Saidi, Noor Baity Song, Lai Kok Yusof, Mohd Termizi Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight |
| title | Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight |
| title_full | Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight |
| title_fullStr | Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight |
| title_full_unstemmed | Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight |
| title_short | Optimization of enzymatic hydrolysis conditions for antimicrobial activity against Pantoea spp. causing rice leaf blight |
| title_sort | optimization of enzymatic hydrolysis conditions for antimicrobial activity against pantoea spp causing rice leaf blight |
| url | http://psasir.upm.edu.my/id/eprint/114783/1/114783.pdf |
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