Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere
Laboratory study was conducted to characterize the beneficial properties of plant growth-promoting rhizobacterial (PGPR) strains isolated from sweet potato rhizosphere. Fifteen rhizobacterial (PGPR) strains were screened for indole acetic acid (IAA) production with and without addition of the precur...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11491/1/Characterization_of_beneficial_properties.pdf |
| _version_ | 1796968089270616064 |
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| author | Yasmin, Farzana Othman, Radziah Sijam, Kamaruzaman Saad, Mohd Said |
| author_facet | Yasmin, Farzana Othman, Radziah Sijam, Kamaruzaman Saad, Mohd Said |
| author_sort | Yasmin, Farzana |
| collection | UPM |
| description | Laboratory study was conducted to characterize the beneficial properties of plant growth-promoting rhizobacterial (PGPR) strains isolated from sweet potato rhizosphere. Fifteen rhizobacterial (PGPR) strains were screened for indole acetic acid (IAA) production with and without addition of the precursor L-tryptophan (L-TRP), phosphate-solubilizing activity, and nitrogen production, antagonistic activity against fungal pathogens, siderophore production and intrinsic antibiotic resistance. Results of the laboratory study showed that 15 rhizobacterial isolates were able to produce indole acetic acid (IAA). The concentration of IAA produced ranged from 3.84 - 13.33mg L-1. Addition of L-tryptophan (L-TRP) to the bacterial isolates increased the production of IAA ranging from 4.94 - 46.66 mg L-1. Six isolates (40%) were able to solubilize insoluble phosphate as evident by production of clear zone on calcium phosphate medium. All isolates were able to grow in N-free media indicating their abilities to produce nitrogen which ranged from 0.74 - 1.32 ppm. Three of the isolates produced fluorescent pigment on agar plate indicated their abilities to produce siderophores. Four isolates were able to inhibit the fungal pathogens Rhizoctonia sp.and Pythium sp. The intrinsic antibiotic test showed that all isolates were resistant against chloramphenicol (10 and 30 µg mL-1), Streptomycin (10 µg mL-1), Kanamycin (5 and 30 µg mL-1), Penicillin (10 µg mL-1) and tetracyclin (30 µg mL-1). The rhizobacterial isolates showed the several beneficial traits that could improve plant growth. |
| first_indexed | 2024-03-06T07:23:18Z |
| format | Article |
| id | upm.eprints-11491 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:23:18Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-114912017-11-20T03:03:03Z http://psasir.upm.edu.my/id/eprint/11491/ Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere Yasmin, Farzana Othman, Radziah Sijam, Kamaruzaman Saad, Mohd Said Laboratory study was conducted to characterize the beneficial properties of plant growth-promoting rhizobacterial (PGPR) strains isolated from sweet potato rhizosphere. Fifteen rhizobacterial (PGPR) strains were screened for indole acetic acid (IAA) production with and without addition of the precursor L-tryptophan (L-TRP), phosphate-solubilizing activity, and nitrogen production, antagonistic activity against fungal pathogens, siderophore production and intrinsic antibiotic resistance. Results of the laboratory study showed that 15 rhizobacterial isolates were able to produce indole acetic acid (IAA). The concentration of IAA produced ranged from 3.84 - 13.33mg L-1. Addition of L-tryptophan (L-TRP) to the bacterial isolates increased the production of IAA ranging from 4.94 - 46.66 mg L-1. Six isolates (40%) were able to solubilize insoluble phosphate as evident by production of clear zone on calcium phosphate medium. All isolates were able to grow in N-free media indicating their abilities to produce nitrogen which ranged from 0.74 - 1.32 ppm. Three of the isolates produced fluorescent pigment on agar plate indicated their abilities to produce siderophores. Four isolates were able to inhibit the fungal pathogens Rhizoctonia sp.and Pythium sp. The intrinsic antibiotic test showed that all isolates were resistant against chloramphenicol (10 and 30 µg mL-1), Streptomycin (10 µg mL-1), Kanamycin (5 and 30 µg mL-1), Penicillin (10 µg mL-1) and tetracyclin (30 µg mL-1). The rhizobacterial isolates showed the several beneficial traits that could improve plant growth. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11491/1/Characterization_of_beneficial_properties.pdf Yasmin, Farzana and Othman, Radziah and Sijam, Kamaruzaman and Saad, Mohd Said (2009) Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere. African Journal of Microbiology Research, 3 (11). art. no. B03FCB214835. pp. 815-821. ISSN 1996-0808 http://www.academicjournals.org/journal/AJMR/article-abstract/B03FCB214835 |
| spellingShingle | Yasmin, Farzana Othman, Radziah Sijam, Kamaruzaman Saad, Mohd Said Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere |
| title | Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere |
| title_full | Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere |
| title_fullStr | Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere |
| title_full_unstemmed | Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere |
| title_short | Characterization of beneficial properties of plant growth-promoting rhizobacteria isolated from sweet potato rhizosphere |
| title_sort | characterization of beneficial properties of plant growth promoting rhizobacteria isolated from sweet potato rhizosphere |
| url | http://psasir.upm.edu.my/id/eprint/11491/1/Characterization_of_beneficial_properties.pdf |
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