Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata
Bacterial cellulose (BC) is a biopolymer synthesized extracellularly by certain bacteria through the polymerization of glucose monomers. This study aimed to produce BC using Enterobacter chuandaensis with fruit extracts from Phoenix dactylifera (D) and Musa acuminata (M) as carbon sources. Attenuate...
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Springer Science and Business Media Deutschland GmbH
2024
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| author | AL-Hasabe, Ashraf Sami Hassan Abdull Razis, Ahmad Faizal Baharum, Nadiya Akmal Yu, Choo Yee Mat Isa, Nurulfiza |
| author_facet | AL-Hasabe, Ashraf Sami Hassan Abdull Razis, Ahmad Faizal Baharum, Nadiya Akmal Yu, Choo Yee Mat Isa, Nurulfiza |
| author_sort | AL-Hasabe, Ashraf Sami Hassan |
| collection | UPM |
| description | Bacterial cellulose (BC) is a biopolymer synthesized extracellularly by certain bacteria through the polymerization of glucose monomers. This study aimed to produce BC using Enterobacter chuandaensis with fruit extracts from Phoenix dactylifera (D) and Musa acuminata (M) as carbon sources. Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) showed characteristic cellulose vibrations, while X-ray diffraction (XRD) identified distinct peaks at 15.34°, 19.98°, 22.58°, and 34.6°, confirming the cellulose structure. Whole-genome sequencing of E. chuandaensis identified key genes involved in BC production. The BC produced then exhibited a molecular weight of 1,857,804 g/mol, with yields of 2.8 g/L and 2.5 g/L for treatments D and M, respectively. The crystallinity index of the purified BC was 74.1, and 13C NMR analysis confirmed the dominant cellulose Iα crystalline form. The BC showed high biocompatibility in cytotoxicity assays, with cell viability between 92% and 100%, indicating its potential for use in biomedical applications. This investigation represents the first report of BC production by E. chuandaensis, which promises a new avenue for sustainable and efficient BC synthesis using fruit extracts as carbon sources. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. |
| first_indexed | 2025-02-19T02:51:37Z |
| format | Article |
| id | upm.eprints-115004 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-02-19T02:51:37Z |
| publishDate | 2024 |
| publisher | Springer Science and Business Media Deutschland GmbH |
| record_format | dspace |
| spelling | upm.eprints-1150042025-02-14T03:55:36Z http://psasir.upm.edu.my/id/eprint/115004/ Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata AL-Hasabe, Ashraf Sami Hassan Abdull Razis, Ahmad Faizal Baharum, Nadiya Akmal Yu, Choo Yee Mat Isa, Nurulfiza Bacterial cellulose (BC) is a biopolymer synthesized extracellularly by certain bacteria through the polymerization of glucose monomers. This study aimed to produce BC using Enterobacter chuandaensis with fruit extracts from Phoenix dactylifera (D) and Musa acuminata (M) as carbon sources. Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) showed characteristic cellulose vibrations, while X-ray diffraction (XRD) identified distinct peaks at 15.34°, 19.98°, 22.58°, and 34.6°, confirming the cellulose structure. Whole-genome sequencing of E. chuandaensis identified key genes involved in BC production. The BC produced then exhibited a molecular weight of 1,857,804 g/mol, with yields of 2.8 g/L and 2.5 g/L for treatments D and M, respectively. The crystallinity index of the purified BC was 74.1, and 13C NMR analysis confirmed the dominant cellulose Iα crystalline form. The BC showed high biocompatibility in cytotoxicity assays, with cell viability between 92% and 100%, indicating its potential for use in biomedical applications. This investigation represents the first report of BC production by E. chuandaensis, which promises a new avenue for sustainable and efficient BC synthesis using fruit extracts as carbon sources. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Science and Business Media Deutschland GmbH 2024 Article PeerReviewed AL-Hasabe, Ashraf Sami Hassan and Abdull Razis, Ahmad Faizal and Baharum, Nadiya Akmal and Yu, Choo Yee and Mat Isa, Nurulfiza (2024) Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata. Archives of Microbiology, 206 (11). art. no. 447. ISSN 0302-8933; eISSN: 1432-072X https://link.springer.com/article/10.1007/s00203-024-04182-2?error=cookies_not_supported&code=40506060-176c-4bc9-8a02-74f7072aa095 10.1007/s00203-024-04182-2 |
| spellingShingle | AL-Hasabe, Ashraf Sami Hassan Abdull Razis, Ahmad Faizal Baharum, Nadiya Akmal Yu, Choo Yee Mat Isa, Nurulfiza Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata |
| title | Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata |
| title_full | Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata |
| title_fullStr | Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata |
| title_full_unstemmed | Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata |
| title_short | Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata |
| title_sort | production and characterization of bacterial cellulose synthesized by enterobacter chuandaensis strain aec using phoenix dactylifera and musa acuminata |
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