Recent insight into the advances and prospects of microbial lipases and their potential applications in industry
Enzymes play a crucial role in various industrial sectors. These biocatalysts not only ensure sustainability and safety but also enhance process efficiency through their unique specificity. Lipases possess versatility as biocatalysts and find utilization in diverse bioconversion reactions. Presently...
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| Format: | Article |
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Hemeroteca Científica Catalana
2024
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| author | Eskandari, Azadeh Thean, Chor Leow Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya |
| author_facet | Eskandari, Azadeh Thean, Chor Leow Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya |
| author_sort | Eskandari, Azadeh |
| collection | UPM |
| description | Enzymes play a crucial role in various industrial sectors. These biocatalysts not only ensure sustainability and safety but also enhance process efficiency through their unique specificity. Lipases possess versatility as biocatalysts and find utilization in diverse bioconversion reactions. Presently, microbial lipases are gaining significant focus owing to the rapid progress in enzyme technology and their widespread implementation in multiple industrial procedures. This updated review presents new knowledge about various origins of microbial lipases, such as fungi, bacteria, and yeast. It highlights both the traditional and modern purification methods, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, the aqueous two-phase system (ATPS), and aqueous two-phase flotation (ATPF), moreover, delves into the diverse applications of microbial lipases across several industries, such as food, vitamin esters, textile, detergent, biodiesel, and bioremediation. Furthermore, the present research unveils the obstacles encountered in employing lipase, the patterns observed in lipase engineering, and the application of CRISPR/Cas genome editing technology for altering the genes responsible for lipase production. Additionally, the immobilization of microorganisms’ lipases onto various carriers also contributes to enhancing the effectiveness and efficiencies of lipases in terms of their catalytic activities. This is achieved by boosting their resilience to heat and ionic conditions (such as inorganic solvents, high-level pH, and temperature). The process also facilitates the ease of recycling them and enables a more concentrated deposition of the enzyme onto the supporting material. Consequently, these characteristics have demonstrated their suitability for application as biocatalysts in diverse industries. |
| first_indexed | 2025-02-19T02:51:39Z |
| format | Article |
| id | upm.eprints-115015 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-02-19T02:51:39Z |
| publishDate | 2024 |
| publisher | Hemeroteca Científica Catalana |
| record_format | dspace |
| spelling | upm.eprints-1150152025-02-17T04:08:08Z http://psasir.upm.edu.my/id/eprint/115015/ Recent insight into the advances and prospects of microbial lipases and their potential applications in industry Eskandari, Azadeh Thean, Chor Leow Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya Enzymes play a crucial role in various industrial sectors. These biocatalysts not only ensure sustainability and safety but also enhance process efficiency through their unique specificity. Lipases possess versatility as biocatalysts and find utilization in diverse bioconversion reactions. Presently, microbial lipases are gaining significant focus owing to the rapid progress in enzyme technology and their widespread implementation in multiple industrial procedures. This updated review presents new knowledge about various origins of microbial lipases, such as fungi, bacteria, and yeast. It highlights both the traditional and modern purification methods, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, the aqueous two-phase system (ATPS), and aqueous two-phase flotation (ATPF), moreover, delves into the diverse applications of microbial lipases across several industries, such as food, vitamin esters, textile, detergent, biodiesel, and bioremediation. Furthermore, the present research unveils the obstacles encountered in employing lipase, the patterns observed in lipase engineering, and the application of CRISPR/Cas genome editing technology for altering the genes responsible for lipase production. Additionally, the immobilization of microorganisms’ lipases onto various carriers also contributes to enhancing the effectiveness and efficiencies of lipases in terms of their catalytic activities. This is achieved by boosting their resilience to heat and ionic conditions (such as inorganic solvents, high-level pH, and temperature). The process also facilitates the ease of recycling them and enables a more concentrated deposition of the enzyme onto the supporting material. Consequently, these characteristics have demonstrated their suitability for application as biocatalysts in diverse industries. Hemeroteca Científica Catalana 2024-03-15 Article PeerReviewed Eskandari, Azadeh and Thean, Chor Leow and Abdul Rahman, Mohd Basyaruddin and Oslan, Siti Nurbaya (2024) Recent insight into the advances and prospects of microbial lipases and their potential applications in industry. International Microbiology, 27 (6). pp. 1597-1631. ISSN 1139-6709; eISSN: 1618-1905 https://link.springer.com/article/10.1007/s10123-024-00498-7?error=cookies_not_supported&code=257052f8-c29a-44f5-8898-3afb8a2d1fa2 10.1007/s10123-024-00498-7 |
| spellingShingle | Eskandari, Azadeh Thean, Chor Leow Abdul Rahman, Mohd Basyaruddin Oslan, Siti Nurbaya Recent insight into the advances and prospects of microbial lipases and their potential applications in industry |
| title | Recent insight into the advances and prospects of microbial lipases and their potential applications in industry |
| title_full | Recent insight into the advances and prospects of microbial lipases and their potential applications in industry |
| title_fullStr | Recent insight into the advances and prospects of microbial lipases and their potential applications in industry |
| title_full_unstemmed | Recent insight into the advances and prospects of microbial lipases and their potential applications in industry |
| title_short | Recent insight into the advances and prospects of microbial lipases and their potential applications in industry |
| title_sort | recent insight into the advances and prospects of microbial lipases and their potential applications in industry |
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