Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum
Microbes that can progress plant productivity and health are recognized as PGPR. Rhizobacteria perform a main role in the phytoremediation process by increasing phytoremediation efficacy, thus the need to identify the superior rhizobacteria has been gaining serious attention. In the present study, P...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Ltd
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115058/1/115058.pdf |
| _version_ | 1824452517668323328 |
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| author | Rahman, Md Ekhlasur Shamsuzzaman, S.M. Mahmud, Khairil Uddin, Md Kamal Abd Ghani, Siti Salwa Abd Shukor, Mohd Yunus Chompa, Sayma Serine Akter, Amaily Nabayi, Abba Sadeq, Buraq Musa Halmi, Mohd Izuan Effendi |
| author_facet | Rahman, Md Ekhlasur Shamsuzzaman, S.M. Mahmud, Khairil Uddin, Md Kamal Abd Ghani, Siti Salwa Abd Shukor, Mohd Yunus Chompa, Sayma Serine Akter, Amaily Nabayi, Abba Sadeq, Buraq Musa Halmi, Mohd Izuan Effendi |
| author_sort | Rahman, Md Ekhlasur |
| collection | UPM |
| description | Microbes that can progress plant productivity and health are recognized as PGPR. Rhizobacteria perform a main role in the phytoremediation process by increasing phytoremediation efficacy, thus the need to identify the superior rhizobacteria has been gaining serious attention. In the present study, Pennisetum purpureum were exposed to a series of As-polluted sand including control (0, 5, 20, 40, 60, and 80 mg kg−1) in plastic crates in a greenhouse environment. Four weeks after exposure, the plants could survive with toxicity of As pollution up to 40 mg kg−1. A total of 22 groups of bacterial isolates were confirmed based on the morphological characteristics, biochemical characteristics, and Gram staining. Preliminary and secondary As screening tests were also conducted. Identification was directed utilizing a molecular identification system. After As screening tests, 17 rhizobacterial isolates presented possible tolerance to As. Based on the molecular identification, 9 bacterial isolates were confirmed which were two [28N A UPM (Bacillus subtilis) and 28N U UPM (Bacillus australimaris)] gram-positive and seven [28N H UPM (Burkholderia seminalis), 28N G UPM (Enterobacter cloacae), 28N D UPM (Pseudomonas stutzeri), 28N I UPM (Sphingobacterium thalpophilum), 28N M UPM (Proteus mirabilis), 28N K UPM (Neisseria perflava) and 28N S UPM (Pseudomonas boreopolis)] gram-negative bacteria. All the nine identified rhizobacteria showed excellent results in terms of PGP characters. Among them, 28N U UPM (Bacillus australimaris) performed the highest PGP traits. The findings revealed that these highly As-resistant rhizobacteria have the potential to produce PGP traits and can help P. purpureum growth and productivity. © 2024 Elsevier Ltd |
| first_indexed | 2025-02-19T02:51:47Z |
| format | Article |
| id | upm.eprints-115058 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2025-02-19T02:51:47Z |
| publishDate | 2024 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | upm.eprints-1150582025-02-18T08:30:50Z http://psasir.upm.edu.my/id/eprint/115058/ Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum Rahman, Md Ekhlasur Shamsuzzaman, S.M. Mahmud, Khairil Uddin, Md Kamal Abd Ghani, Siti Salwa Abd Shukor, Mohd Yunus Chompa, Sayma Serine Akter, Amaily Nabayi, Abba Sadeq, Buraq Musa Halmi, Mohd Izuan Effendi Microbes that can progress plant productivity and health are recognized as PGPR. Rhizobacteria perform a main role in the phytoremediation process by increasing phytoremediation efficacy, thus the need to identify the superior rhizobacteria has been gaining serious attention. In the present study, Pennisetum purpureum were exposed to a series of As-polluted sand including control (0, 5, 20, 40, 60, and 80 mg kg−1) in plastic crates in a greenhouse environment. Four weeks after exposure, the plants could survive with toxicity of As pollution up to 40 mg kg−1. A total of 22 groups of bacterial isolates were confirmed based on the morphological characteristics, biochemical characteristics, and Gram staining. Preliminary and secondary As screening tests were also conducted. Identification was directed utilizing a molecular identification system. After As screening tests, 17 rhizobacterial isolates presented possible tolerance to As. Based on the molecular identification, 9 bacterial isolates were confirmed which were two [28N A UPM (Bacillus subtilis) and 28N U UPM (Bacillus australimaris)] gram-positive and seven [28N H UPM (Burkholderia seminalis), 28N G UPM (Enterobacter cloacae), 28N D UPM (Pseudomonas stutzeri), 28N I UPM (Sphingobacterium thalpophilum), 28N M UPM (Proteus mirabilis), 28N K UPM (Neisseria perflava) and 28N S UPM (Pseudomonas boreopolis)] gram-negative bacteria. All the nine identified rhizobacteria showed excellent results in terms of PGP characters. Among them, 28N U UPM (Bacillus australimaris) performed the highest PGP traits. The findings revealed that these highly As-resistant rhizobacteria have the potential to produce PGP traits and can help P. purpureum growth and productivity. © 2024 Elsevier Ltd Elsevier Ltd 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115058/1/115058.pdf Rahman, Md Ekhlasur and Shamsuzzaman, S.M. and Mahmud, Khairil and Uddin, Md Kamal and Abd Ghani, Siti Salwa and Abd Shukor, Mohd Yunus and Chompa, Sayma Serine and Akter, Amaily and Nabayi, Abba and Sadeq, Buraq Musa and Halmi, Mohd Izuan Effendi (2024) Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum. Biocatalysis and Agricultural Biotechnology, 59. art. no. 103237. ISSN 1878-8181; eISSN: 1878-8181 https://linkinghub.elsevier.com/retrieve/pii/S1878818124002214 10.1016/j.bcab.2024.103237 |
| spellingShingle | Rahman, Md Ekhlasur Shamsuzzaman, S.M. Mahmud, Khairil Uddin, Md Kamal Abd Ghani, Siti Salwa Abd Shukor, Mohd Yunus Chompa, Sayma Serine Akter, Amaily Nabayi, Abba Sadeq, Buraq Musa Halmi, Mohd Izuan Effendi Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum |
| title | Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum |
| title_full | Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum |
| title_fullStr | Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum |
| title_full_unstemmed | Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum |
| title_short | Isolation, screening, identification, and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from Pennisetum purpureum |
| title_sort | isolation screening identification and characterization of arsenic resistant plant growth promoting rhizobacteria isolated from pennisetum purpureum |
| url | http://psasir.upm.edu.my/id/eprint/115058/1/115058.pdf |
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