Cloning and expression of the HN gene from the velogenic viscerotropic Newcastle disease virus strain AF2240 in Sf9 insect cells
The haemagglutinin-neuraminidase (HN) gene of Newcastle disease virus (NDV) strain AF2240, amplified from the viral genomic RNA (~1.8 kb) was directionally cloned and inserted into a baculovirus expression vector system. The recombinant glycoprotein expressed in Spodoptera frugiperda (Sf9) cells sho...
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Springer Science and Business Media LLC
2000
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