Development of a modified method for sequencing high G:C regions in chicken anemia virus genome
Two areas in the chicken anemia virus (CAV) genome have high G:C content with secondary structures. These two G:C rich areas could not be sequenced with Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit. Several modifications were carried out to solve the problem. Finally, a package of mod...
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| Format: | Article |
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Taylor & Francis
2002
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| author | Chowdhury, S.M.Z.H. Omar, A.R. Aini, I. Hair-Bejo, M. |
| author_facet | Chowdhury, S.M.Z.H. Omar, A.R. Aini, I. Hair-Bejo, M. |
| author_sort | Chowdhury, S.M.Z.H. |
| collection | UPM |
| description | Two areas in the chicken anemia virus (CAV) genome have high G:C content with secondary structures. These two G:C rich areas could not be sequenced with Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit. Several modifications were carried out to solve the problem. Finally, a package of modified method was developed to sequence the high G:C areas. The result showed that the Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit with the normal procedures are not suitable for sequencing the high G:C regions of the CAV genome. The present developed method made the Perkin Elmer's Kit useful for the first time to sequence the G:C rich hairpin structures of the CAV genome. The system may be useful to sequence all other G:C rich DNA templates. |
| first_indexed | 2025-03-07T13:05:01Z |
| format | Article |
| id | upm.eprints-115317 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2025-03-07T13:05:01Z |
| publishDate | 2002 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-1153172025-03-03T02:56:37Z http://psasir.upm.edu.my/id/eprint/115317/ Development of a modified method for sequencing high G:C regions in chicken anemia virus genome Chowdhury, S.M.Z.H. Omar, A.R. Aini, I. Hair-Bejo, M. Two areas in the chicken anemia virus (CAV) genome have high G:C content with secondary structures. These two G:C rich areas could not be sequenced with Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit. Several modifications were carried out to solve the problem. Finally, a package of modified method was developed to sequence the high G:C areas. The result showed that the Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit with the normal procedures are not suitable for sequencing the high G:C regions of the CAV genome. The present developed method made the Perkin Elmer's Kit useful for the first time to sequence the G:C rich hairpin structures of the CAV genome. The system may be useful to sequence all other G:C rich DNA templates. Taylor & Francis 2002 Article PeerReviewed Chowdhury, S.M.Z.H. and Omar, A.R. and Aini, I. and Hair-Bejo, M. (2002) Development of a modified method for sequencing high G:C regions in chicken anemia virus genome. Journal of Biochemistry, Molecular Biology and Biophysics, 6 (3). pp. 229-232. ISSN 1025-8140; eISSN: 1025-8140 https://taylorandfrancis.com/ 10.1080/10258140290022310 |
| spellingShingle | Chowdhury, S.M.Z.H. Omar, A.R. Aini, I. Hair-Bejo, M. Development of a modified method for sequencing high G:C regions in chicken anemia virus genome |
| title | Development of a modified method for sequencing high G:C regions in chicken anemia virus genome |
| title_full | Development of a modified method for sequencing high G:C regions in chicken anemia virus genome |
| title_fullStr | Development of a modified method for sequencing high G:C regions in chicken anemia virus genome |
| title_full_unstemmed | Development of a modified method for sequencing high G:C regions in chicken anemia virus genome |
| title_short | Development of a modified method for sequencing high G:C regions in chicken anemia virus genome |
| title_sort | development of a modified method for sequencing high g c regions in chicken anemia virus genome |
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