Functional expression, purification, biochemical and biophysical characterizations, and molecular dynamics simulation of a histidine acid phosphatase from Saccharomyces cerevisiae

Functional expression, purification, biochemical and biophysical characterizations, and molecular dynamics simulation of a histidine acid phosphatase from Saccharomyces cerevisiae

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A histidine acid phosphatase (HAP) (PhySc) with 99.50% protein sequence similarity with PHO5 from Saccharomyces cerevisiae was expressed functionally with the molecular mass of ∼110 kDa through co-expression along with the set of molecular chaperones dnaK, dnaJ, GroESL. The purified HAP illustrated...

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Bibliographic Details
Main Authors: Nezhad, Nima Ghahremani, Jamaludin, Siti Zahra, Raja Abd Rahman, Raja Noor Zaliha, Mohd Yahaya, Normi, Oslan, Siti Nurbaya, Mohd Shariff, Fairolniza, Mat Isa, Nurulfiza, Leow, Thean Chor
Format: Article
Language:English
Published: Springer Science and Business Media 2024
Online Access:http://psasir.upm.edu.my/id/eprint/115409/1/115409.pdf

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