Development And Isolation Of Dna Microsatellite Markers For The Characterisation And Identification Of Mystus Nemurus (C & V)
Mystus nemurus or better known as 'ikan Baung' is an indigenous species In Malaysia. The popularity of this freshwater fish among the locals had made it an economically important aquaculture candidate. Of the so many DNA marker systems available, microsatellites are particularly useful...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11657/1/FSAS_2003_36.pdf |
| Summary: | Mystus nemurus or better known as 'ikan Baung' is an indigenous species In
Malaysia. The popularity of this freshwater fish among the locals had made it an
economically important aquaculture candidate. Of the so many DNA marker systems
available, microsatellites are particularly useful for population studies and have been
proven to be the most efficient due to their codominant modes of inheritance.
Two methods were used to isolate microsatellites in M. nemurus, namely Random
Amplified Hybridisation Microsatellites (RAHMs) and Random Amplified
Microsatellites (RAMs). A total of 88 micro satellite sequences and 18 cryptic simple
regions were produced. The majority (90%) of these microsatellites were detected by
the RAMs method while the RAHMs method required further optimisation and
modification. The isolation of microsatellites resulted in 33 primer pairs being
designed. |
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