Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate
The performance of direct fermentation of sago starch to ethanol by three strains of genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase), YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and glucoamylase), was investigated. In fermentation using 2 L...
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| Format: | Thesis |
| Language: | English English |
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2001
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11856/1/FSMB_2001_39.pdf |
| _version_ | 1811137324848775168 |
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| author | Ang, Dek Chang |
| author_facet | Ang, Dek Chang |
| author_sort | Ang, Dek Chang |
| collection | UPM |
| description | The performance of direct fermentation of sago starch to ethanol by three strains of
genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase),
YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and
glucoamylase), was investigated. In fermentation using 2 L fermenter, we found that
strains YKU 107, YKU 131 and YKU 132 was capable to hydrolyse 83.45%,67.45%
and 71.9% of sago starch, respectively. However, only strain YKU 131 has the ability
to produce significant amount of ethanol (2.16 gL-1) from sago starch. The superiority
of strain YKU 131 as compared to strain YKU 107 and YKU 132 was found to be
correlated with its ability to secrete glucoamylase. S. cerevisiae YKU 131 was selected
for further studies. To investigate the performance of S. cerevlszae YKU 131 in ethanol
production using various types of starch, initial starch concentration was fixed at 20
gL-1 Among different types of starch investigated, com starch was the most susceptible
to the glucoamylase, followed by sago, cassava, potato and rice starch. |
| first_indexed | 2024-03-06T07:23:55Z |
| format | Thesis |
| id | upm.eprints-11856 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-09-25T03:32:30Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | upm.eprints-118562024-06-26T09:04:30Z http://psasir.upm.edu.my/id/eprint/11856/ Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate Ang, Dek Chang The performance of direct fermentation of sago starch to ethanol by three strains of genetically modified Saccharomyces cerevisiae, YKU 107 (expressing a.-amylase), YKU 131 (expressing glucoamylase) and YKU 132 (expressing a.-amylase and glucoamylase), was investigated. In fermentation using 2 L fermenter, we found that strains YKU 107, YKU 131 and YKU 132 was capable to hydrolyse 83.45%,67.45% and 71.9% of sago starch, respectively. However, only strain YKU 131 has the ability to produce significant amount of ethanol (2.16 gL-1) from sago starch. The superiority of strain YKU 131 as compared to strain YKU 107 and YKU 132 was found to be correlated with its ability to secrete glucoamylase. S. cerevisiae YKU 131 was selected for further studies. To investigate the performance of S. cerevlszae YKU 131 in ethanol production using various types of starch, initial starch concentration was fixed at 20 gL-1 Among different types of starch investigated, com starch was the most susceptible to the glucoamylase, followed by sago, cassava, potato and rice starch. 2001-06 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/11856/1/FSMB_2001_39.pdf Ang, Dek Chang (2001) Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate. Masters thesis, Universiti Putra Malaysia. Saccharomyces cerevisiae. Transgenic organisms. Fermentation. English |
| spellingShingle | Saccharomyces cerevisiae. Transgenic organisms. Fermentation. Ang, Dek Chang Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate |
| title | Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate |
| title_full | Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate |
| title_fullStr | Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate |
| title_full_unstemmed | Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate |
| title_short | Production Of Ethanol By Genetically Modified Saccharomyces Cerevisiae Using Sago Starch As Substrate |
| title_sort | production of ethanol by genetically modified saccharomyces cerevisiae using sago starch as substrate |
| topic | Saccharomyces cerevisiae. Transgenic organisms. Fermentation. |
| url | http://psasir.upm.edu.my/id/eprint/11856/1/FSMB_2001_39.pdf |
| work_keys_str_mv | AT angdekchang productionofethanolbygeneticallymodifiedsaccharomycescerevisiaeusingsagostarchassubstrate |