Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers
Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein compone...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8.pdf |
| _version_ | 1825944951390732288 |
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| author | Teo, Chee How |
| author_facet | Teo, Chee How |
| author_sort | Teo, Chee How |
| collection | UPM |
| description | Retrotransposons are the most common class of eukaryotic transposable elements
that make up over 50% of nuclear DNA found in many plants with large and
complex genomes. The long terminal repeat (LTR) retrotransposons have a high
degree of autonomy and encode at least five distinct protein components, which are
required for their movement in the genome. We have exploited the repetitive,
dispersed nature of many LTR-containing retrotransposon families for the
visualization of genomic polymorphism (genome constitution) and the visualization
of varieties polymorphism (varieties classification). The IRAP-PCR (Inter-
Retrotransposon Amplified Polymorphism-Polymerase Chain Reaction) markers are
generated by the proximity of two LTRs or reverse transcriptase CRT) genes using
outward-facing primers annealing to LTR target sequence. This method could
distinguish between banana genome constitution (A and B genome) and varieties.
This method can track new insertion of retrotransposon in banana tissue culture
materials. The evolution of Malaysian bananas can also be tracked using this
phylogenetic analysis. Since the method is PCR-based, it may allow the detection
and isolation of the active, newly inserted retrotransposon copies easily, by analyzing the subsequent generations within an otherwise genetically homogeneous material.
This method is applicable to other plants with dispersed families of LTR retrotransposons
where either the LTR or nearby internal sequences are known. |
| first_indexed | 2024-03-06T07:23:56Z |
| format | Thesis |
| id | upm.eprints-11859 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-09-25T03:32:30Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | upm.eprints-118592024-06-27T01:30:55Z http://psasir.upm.edu.my/id/eprint/11859/ Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers Teo, Chee How Retrotransposons are the most common class of eukaryotic transposable elements that make up over 50% of nuclear DNA found in many plants with large and complex genomes. The long terminal repeat (LTR) retrotransposons have a high degree of autonomy and encode at least five distinct protein components, which are required for their movement in the genome. We have exploited the repetitive, dispersed nature of many LTR-containing retrotransposon families for the visualization of genomic polymorphism (genome constitution) and the visualization of varieties polymorphism (varieties classification). The IRAP-PCR (Inter- Retrotransposon Amplified Polymorphism-Polymerase Chain Reaction) markers are generated by the proximity of two LTRs or reverse transcriptase CRT) genes using outward-facing primers annealing to LTR target sequence. This method could distinguish between banana genome constitution (A and B genome) and varieties. This method can track new insertion of retrotransposon in banana tissue culture materials. The evolution of Malaysian bananas can also be tracked using this phylogenetic analysis. Since the method is PCR-based, it may allow the detection and isolation of the active, newly inserted retrotransposon copies easily, by analyzing the subsequent generations within an otherwise genetically homogeneous material. This method is applicable to other plants with dispersed families of LTR retrotransposons where either the LTR or nearby internal sequences are known. 2002-04 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8.pdf Teo, Chee How (2002) Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers. Masters thesis, Universiti Putra Malaysia. Bananas - Malaysia Food technology English |
| spellingShingle | Bananas - Malaysia Food technology Teo, Chee How Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers |
| title | Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers |
| title_full | Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers |
| title_fullStr | Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers |
| title_full_unstemmed | Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers |
| title_short | Classification Of The Malaysian Banana Varieties Using Retrotransposon Markers |
| title_sort | classification of the malaysian banana varieties using retrotransposon markers |
| topic | Bananas - Malaysia Food technology |
| url | http://psasir.upm.edu.my/id/eprint/11859/1/FSMB_2002_8.pdf |
| work_keys_str_mv | AT teocheehow classificationofthemalaysianbananavarietiesusingretrotransposonmarkers |