Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
The use of probiotics as an alternative to antibiotic growth promoter has gained popularity in the commercial poultry industry in view of the hazards posed by antibiotics to human and animal health. However, the science behind the probiotic microorganisms has been poor with respect to their ident...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12059/1/IB_2009_16_A.pdf |
| _version_ | 1796968162093170688 |
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| author | Lee, Chin Mei |
| author_facet | Lee, Chin Mei |
| author_sort | Lee, Chin Mei |
| collection | UPM |
| description | The use of probiotics as an alternative to antibiotic growth promoter has gained
popularity in the commercial poultry industry in view of the hazards posed by
antibiotics to human and animal health. However, the science behind the probiotic
microorganisms has been poor with respect to their identity at the species and strain
level, their interaction with the host animal, and their efficacy in poultry practices.
Thus, a reliable and efficient method is essential to monitor the probiotic
microorganisms and to perform quality control of commercial probiotic products. In
the present study, molecular methods were applied for reidentification,
characterisation and enumeration of 12 probiotic Lactobacillus strains which were
previously identified with classical biochemical tests.
Based on comparative sequence analyses of the 16S ribosomal RNA (rRNA) gene
and 16S-23S rRNA gene intergenic spacer region (ISR), discrepancies were found in
the identification of nine out of the 12 Lactobacillus strains, namely, L. brevis C 1, L.
brevis C 10, L. fermentum C 16, L. brevis C 17, L. crispatus I12, L. acidophilus I 16, L. fermentum I 24, L. fermentum I 25 and L. acidophilus I 26. These strains were
reidentified as L. reuteri C 1, L. reuteri C 10, L. reuteri C 16, L. panis C 17, L. brevis
I 12, L. gallinarum I 16, L. salivarius I 24, L. brevis I 25 and L. gallinarum I 26. The
rate of misidentification is high when conventional identification methods are used. |
| first_indexed | 2024-03-06T07:24:23Z |
| format | Thesis |
| id | upm.eprints-12059 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:24:23Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | upm.eprints-120592013-05-27T07:50:56Z http://psasir.upm.edu.my/id/eprint/12059/ Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry Lee, Chin Mei The use of probiotics as an alternative to antibiotic growth promoter has gained popularity in the commercial poultry industry in view of the hazards posed by antibiotics to human and animal health. However, the science behind the probiotic microorganisms has been poor with respect to their identity at the species and strain level, their interaction with the host animal, and their efficacy in poultry practices. Thus, a reliable and efficient method is essential to monitor the probiotic microorganisms and to perform quality control of commercial probiotic products. In the present study, molecular methods were applied for reidentification, characterisation and enumeration of 12 probiotic Lactobacillus strains which were previously identified with classical biochemical tests. Based on comparative sequence analyses of the 16S ribosomal RNA (rRNA) gene and 16S-23S rRNA gene intergenic spacer region (ISR), discrepancies were found in the identification of nine out of the 12 Lactobacillus strains, namely, L. brevis C 1, L. brevis C 10, L. fermentum C 16, L. brevis C 17, L. crispatus I12, L. acidophilus I 16, L. fermentum I 24, L. fermentum I 25 and L. acidophilus I 26. These strains were reidentified as L. reuteri C 1, L. reuteri C 10, L. reuteri C 16, L. panis C 17, L. brevis I 12, L. gallinarum I 16, L. salivarius I 24, L. brevis I 25 and L. gallinarum I 26. The rate of misidentification is high when conventional identification methods are used. 2009-12 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12059/1/IB_2009_16_A.pdf Lee, Chin Mei (2009) Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry. Masters thesis, Universiti Putra Malaysia. Probiotics Lactobacillus - Molecular aspects Poultry English |
| spellingShingle | Probiotics Lactobacillus - Molecular aspects Poultry Lee, Chin Mei Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry |
| title | Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
|
| title_full | Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
|
| title_fullStr | Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
|
| title_full_unstemmed | Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
|
| title_short | Molecular Approaches For Identification, Characterisation And Quantification Of Probiotic Lactobacillus Strains For Poultry
|
| title_sort | molecular approaches for identification characterisation and quantification of probiotic lactobacillus strains for poultry |
| topic | Probiotics Lactobacillus - Molecular aspects Poultry |
| url | http://psasir.upm.edu.my/id/eprint/12059/1/IB_2009_16_A.pdf |
| work_keys_str_mv | AT leechinmei molecularapproachesforidentificationcharacterisationandquantificationofprobioticlactobacillusstrainsforpoultry |