Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
A study on the characteristics of the Malaysian field and vaccine strains of Pasteurella multocida type B was conducted . In addition , the immunogenic potential of haemorrhagic septicaemia ( HS ) vaccines was evaluated in buffaloes . A total of 48 P. multocida isolates including the five HS va...
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English English |
| Published: |
1993
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/12302/1/FPV_1993_1_A.pdf |
| _version_ | 1825945026539028480 |
|---|---|
| author | Subramaniam, Chandrasekaran |
| author_facet | Subramaniam, Chandrasekaran |
| author_sort | Subramaniam, Chandrasekaran |
| collection | UPM |
| description | A study on the characteristics of the Malaysian field and
vaccine strains of Pasteurella multocida type B was conducted .
In addition , the immunogenic potential of haemorrhagic
septicaemia ( HS ) vaccines was evaluated in buffaloes .
A total of 48 P. multocida isolates including the five HS
vaccine strains were included in the study . Initial studies
showed significant cross-protection in mice amongst the five
vaccine strains . The 48 isolates , when subjected to
biochemical tests had very little or no variability amongst
them. Type-specific hyper immune sera were prepared in rabbits
and buffaloes . The sera were then absorbed using antigens
prepared from the 48 isolates . A series of serological tests
were conducted using the pre- and post-absorbed sera and the
antigen preparations . The protein profile of the five vaccine
and five randomly selected field strains of this organism
revealed that there appeared to be no variation amongst the
field and vaccine strains .
The antibody responses of buffaloes immunised with three
conventional HS vaccines viz . , broth bacterin ( BB ) ,
alum-precipitated vaccine ( APV ) and oil adjuvant vaccine ( OAV )
and one experimental double emulsion vaccine ( DEV ) were
determined by an enzyme-linked immunosorbent assay ( ELISA ) .
Antibody levels were significantly higher in buffaloes
immunised with the adjuvanted vaccines ( APV , OAV , DEV ) .
Buffaloes immunised with BB alone were protected at 6 weeks but
not at 12 weeks post-immunisation . Buffaloes immunised with
APV were protected at 24 weeks but only partially at 52 weeks
post-immunisation . On the other hand , buffaloes immunised with
OAV or DEV were fully protected at 52 weeks but only partially
at 76 weeks post-immunisation .There was also a positive
relationship between the ELISA titres and protection in
buffaloes .
|
| first_indexed | 2024-03-06T07:25:06Z |
| format | Thesis |
| id | upm.eprints-12302 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:25:06Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | upm.eprints-123022011-09-07T07:32:59Z http://psasir.upm.edu.my/id/eprint/12302/ Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes Subramaniam, Chandrasekaran A study on the characteristics of the Malaysian field and vaccine strains of Pasteurella multocida type B was conducted . In addition , the immunogenic potential of haemorrhagic septicaemia ( HS ) vaccines was evaluated in buffaloes . A total of 48 P. multocida isolates including the five HS vaccine strains were included in the study . Initial studies showed significant cross-protection in mice amongst the five vaccine strains . The 48 isolates , when subjected to biochemical tests had very little or no variability amongst them. Type-specific hyper immune sera were prepared in rabbits and buffaloes . The sera were then absorbed using antigens prepared from the 48 isolates . A series of serological tests were conducted using the pre- and post-absorbed sera and the antigen preparations . The protein profile of the five vaccine and five randomly selected field strains of this organism revealed that there appeared to be no variation amongst the field and vaccine strains . The antibody responses of buffaloes immunised with three conventional HS vaccines viz . , broth bacterin ( BB ) , alum-precipitated vaccine ( APV ) and oil adjuvant vaccine ( OAV ) and one experimental double emulsion vaccine ( DEV ) were determined by an enzyme-linked immunosorbent assay ( ELISA ) . Antibody levels were significantly higher in buffaloes immunised with the adjuvanted vaccines ( APV , OAV , DEV ) . Buffaloes immunised with BB alone were protected at 6 weeks but not at 12 weeks post-immunisation . Buffaloes immunised with APV were protected at 24 weeks but only partially at 52 weeks post-immunisation . On the other hand , buffaloes immunised with OAV or DEV were fully protected at 52 weeks but only partially at 76 weeks post-immunisation .There was also a positive relationship between the ELISA titres and protection in buffaloes . 1993-06 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12302/1/FPV_1993_1_A.pdf Subramaniam, Chandrasekaran (1993) Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes. Masters thesis, Universiti Pertanian Malaysia. English |
| spellingShingle | Subramaniam, Chandrasekaran Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes |
| title | Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
|
| title_full | Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
|
| title_fullStr | Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
|
| title_full_unstemmed | Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
|
| title_short | Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes
|
| title_sort | pasteurella multocida type b in malaysia characterisation immune response and protection in vaccinated buffaloes |
| url | http://psasir.upm.edu.my/id/eprint/12302/1/FPV_1993_1_A.pdf |
| work_keys_str_mv | AT subramaniamchandrasekaran pasteurellamultocidatypebinmalaysiacharacterisationimmuneresponseandprotectioninvaccinatedbuffaloes |