Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
A study was conducted to determine if goat ovaries obtained from a slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae, (Gelidiella acerosa), with a chemical structure similar to h...
| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
1997
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/12327/1/FPV_1997_6_A.pdf |
| _version_ | 1825945031396032512 |
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| author | Chandrasiri, Alawattage Don Nimal |
| author_facet | Chandrasiri, Alawattage Don Nimal |
| author_sort | Chandrasiri, Alawattage Don Nimal |
| collection | UPM |
| description | A study was conducted to determine if goat ovaries obtained from a
slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were
conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae,
(Gelidiella acerosa), with a chemical structure similar to heparin could be used for
sperm capacitation.
The acrosome reaction was tested by a dual staining technique (Experiment 1 )
and the zona free hamster penetration test (Experiment 2). In vitro maturation o f goat
oocytes and fertilisation were assessed by the number of zygotes cleaving beyond the
2-cell stage (Experiment 3).
Freshly ejaculated semen from two fertile bucks were used for sperm
capacitation using two defined media (DM-H and DM-Ca). Heparin and S-ACT-l
were tested at concentrations of 10, 20, 50 and 100 ug/ml. After incubating for 15
min. at 39° C with 5 % CO2 in air under maximum humidity, samples were stained
with trypan blue and Giemsa stain (dual stain). The results indicated that the sperm
treated with heparin and S-ACT - 1 showed similar staining characteristics. |
| first_indexed | 2024-03-06T07:25:10Z |
| format | Thesis |
| id | upm.eprints-12327 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:25:10Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | upm.eprints-123272011-09-12T01:34:30Z http://psasir.upm.edu.my/id/eprint/12327/ Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat Chandrasiri, Alawattage Don Nimal A study was conducted to determine if goat ovaries obtained from a slaughterhouse could be used for in vitro fertilisation (lVF). Three experiments were conducted to ascertain if a substance named S-ACT-l, extracted from a marine algae, (Gelidiella acerosa), with a chemical structure similar to heparin could be used for sperm capacitation. The acrosome reaction was tested by a dual staining technique (Experiment 1 ) and the zona free hamster penetration test (Experiment 2). In vitro maturation o f goat oocytes and fertilisation were assessed by the number of zygotes cleaving beyond the 2-cell stage (Experiment 3). Freshly ejaculated semen from two fertile bucks were used for sperm capacitation using two defined media (DM-H and DM-Ca). Heparin and S-ACT-l were tested at concentrations of 10, 20, 50 and 100 ug/ml. After incubating for 15 min. at 39° C with 5 % CO2 in air under maximum humidity, samples were stained with trypan blue and Giemsa stain (dual stain). The results indicated that the sperm treated with heparin and S-ACT - 1 showed similar staining characteristics. 1997-03 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12327/1/FPV_1997_6_A.pdf Chandrasiri, Alawattage Don Nimal (1997) Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat. PhD thesis, Universiti Putra Malaysia. Goats - Fertility Fertilization in vitro English |
| spellingShingle | Goats - Fertility Fertilization in vitro Chandrasiri, Alawattage Don Nimal Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat |
| title | Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
|
| title_full | Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
|
| title_fullStr | Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
|
| title_full_unstemmed | Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
|
| title_short | Extract Of Gelidiella Acerosa (S-Act-1) As A Capacitation Agent For In Vitro Fertilisation In Goat
|
| title_sort | extract of gelidiella acerosa s act 1 as a capacitation agent for in vitro fertilisation in goat |
| topic | Goats - Fertility Fertilization in vitro |
| url | http://psasir.upm.edu.my/id/eprint/12327/1/FPV_1997_6_A.pdf |
| work_keys_str_mv | AT chandrasirialawattagedonnimal extractofgelidiellaacerosasact1asacapacitationagentforinvitrofertilisationingoat |