Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers
Male inflorescences have potential to be used as explants for rapid micropropagation of Musa sp. The male flowers of four banana cultivars, namely ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Abu’ belonging to three genome types in Musa(AAA, AAB, and ABB), were cultured onto Murashige and Skoog (MS) medium...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12399/1/Micropropagation_of_some_Malaysian_banana_and.pdf |
| _version_ | 1825945044101627904 |
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| author | Darvari, Fatemeh Mahdavi Meon, Sariah Abdullah, Mohd Puad Mahmood, Maziah |
| author_facet | Darvari, Fatemeh Mahdavi Meon, Sariah Abdullah, Mohd Puad Mahmood, Maziah |
| author_sort | Darvari, Fatemeh Mahdavi |
| collection | UPM |
| description | Male inflorescences have potential to be used as explants for rapid micropropagation of Musa sp. The male flowers of four banana cultivars, namely ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Abu’ belonging to three genome types in Musa(AAA, AAB, and ABB), were cultured onto Murashige and Skoog (MS) medium which was supplemented with 1 mg/L of TDZ, BAP, Kin, 2-ip and Zea. The number of shoots was found to significantly increase in both TDZ and BAP treatments, as compared to other cytokinins. TDZ at 0.4, 0.6 and 0.8 mg/L, in particular, appeared to be optimum for shoot induction in ‘Berangan-AAA’, ‘Rastali-AAB’ and ‘Nangka-AAB’ and ‘Abu-ABB’, respectively. However, all the cultivars showed their highest response to regeneration at 8 mg/L of BAP. After the initiation of the explants onto the MS media for all the cultivars, the highest number of cauliflower-like bodies’ (CLBs) clusters was observed at two months of culture. The number of induced ‘CLBs’ cluster is dependent on the size of male buds. Male inflorescences with the size of 20 mm were found to induce more ‘CLBs’ clusters. Meanwhile, the number of shoots produced is dependent on both the cytokinins and cultivars used. |
| first_indexed | 2024-03-06T07:25:22Z |
| format | Article |
| id | upm.eprints-12399 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:25:22Z |
| publishDate | 2010 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-123992017-11-09T09:09:12Z http://psasir.upm.edu.my/id/eprint/12399/ Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers Darvari, Fatemeh Mahdavi Meon, Sariah Abdullah, Mohd Puad Mahmood, Maziah Male inflorescences have potential to be used as explants for rapid micropropagation of Musa sp. The male flowers of four banana cultivars, namely ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Abu’ belonging to three genome types in Musa(AAA, AAB, and ABB), were cultured onto Murashige and Skoog (MS) medium which was supplemented with 1 mg/L of TDZ, BAP, Kin, 2-ip and Zea. The number of shoots was found to significantly increase in both TDZ and BAP treatments, as compared to other cytokinins. TDZ at 0.4, 0.6 and 0.8 mg/L, in particular, appeared to be optimum for shoot induction in ‘Berangan-AAA’, ‘Rastali-AAB’ and ‘Nangka-AAB’ and ‘Abu-ABB’, respectively. However, all the cultivars showed their highest response to regeneration at 8 mg/L of BAP. After the initiation of the explants onto the MS media for all the cultivars, the highest number of cauliflower-like bodies’ (CLBs) clusters was observed at two months of culture. The number of induced ‘CLBs’ cluster is dependent on the size of male buds. Male inflorescences with the size of 20 mm were found to induce more ‘CLBs’ clusters. Meanwhile, the number of shoots produced is dependent on both the cytokinins and cultivars used. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12399/1/Micropropagation_of_some_Malaysian_banana_and.pdf Darvari, Fatemeh Mahdavi and Meon, Sariah and Abdullah, Mohd Puad and Mahmood, Maziah (2010) Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers. African Journal of Biotechnology, 9 (16). art. no. 76E98D420962. pp. 2360-2366. ISSN 1684–5315 http://academicjournals.org/journal/AJB/article-abstract/76E98D420962 |
| spellingShingle | Darvari, Fatemeh Mahdavi Meon, Sariah Abdullah, Mohd Puad Mahmood, Maziah Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers |
| title | Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers |
| title_full | Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers |
| title_fullStr | Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers |
| title_full_unstemmed | Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers |
| title_short | Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers |
| title_sort | micropropagation of some malaysian banana and plantain musa sp cultivars using male flowers |
| url | http://psasir.upm.edu.my/id/eprint/12399/1/Micropropagation_of_some_Malaysian_banana_and.pdf |
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