A predominant β-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity
Low reaction yields and the high cost of obtaining a single type of pure CD make γ-CD costly. Using rational design and with the aid of 3D modeling structures, recombinant CGTase from Bacillus sp. G1 was molecularly engineered with the aim of producing a higher percentage of γ-CD. A single mutation...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/12782/1/A%20predominant%20%CE%B2.pdf |