A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography
Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society for Microbiology and Biotechnology
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12790/1/A%20preparative%20purification%20process%20for%20recombinant%20Hepatitis%20B%20core%20antigen%20using%20online%20capture%20by%20expanded%20bed%20adsorption%20followed%20by%20size.pdf |
| _version_ | 1825945111398187008 |
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| author | Ho, Chin Woi Tan, Wen Siang Chong, Fui Chin Ling, Tau Chuan Tey, Beng Ti |
| author_facet | Ho, Chin Woi Tan, Wen Siang Chong, Fui Chin Ling, Tau Chuan Tey, Beng Ti |
| author_sort | Ho, Chin Woi |
| collection | UPM |
| description | Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was first captured by anion exchange expanded bed adsorption chromatography integrated with a cell disruption process. This online capture process has shortened the process time and eliminated the “hold-up” period that may be detrimental to the quality of target protein. The eluted product from the expanded bed adsorption chromatography was subsequently purified using size-exclusion chromatography. The results showed that this novel purification protocol achieved a recovery yield of 45.1% with a product purity of 88.2%, which corresponds to a purification factor of 4.5. The recovered HBcAg is still biologically active as shown by ELISA test. |
| first_indexed | 2024-03-06T07:26:25Z |
| format | Article |
| id | upm.eprints-12790 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:26:25Z |
| publishDate | 2009 |
| publisher | Korean Society for Microbiology and Biotechnology |
| record_format | dspace |
| spelling | upm.eprints-127902015-09-17T00:10:47Z http://psasir.upm.edu.my/id/eprint/12790/ A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography Ho, Chin Woi Tan, Wen Siang Chong, Fui Chin Ling, Tau Chuan Tey, Beng Ti Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was first captured by anion exchange expanded bed adsorption chromatography integrated with a cell disruption process. This online capture process has shortened the process time and eliminated the “hold-up” period that may be detrimental to the quality of target protein. The eluted product from the expanded bed adsorption chromatography was subsequently purified using size-exclusion chromatography. The results showed that this novel purification protocol achieved a recovery yield of 45.1% with a product purity of 88.2%, which corresponds to a purification factor of 4.5. The recovered HBcAg is still biologically active as shown by ELISA test. Korean Society for Microbiology and Biotechnology 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12790/1/A%20preparative%20purification%20process%20for%20recombinant%20Hepatitis%20B%20core%20antigen%20using%20online%20capture%20by%20expanded%20bed%20adsorption%20followed%20by%20size.pdf Ho, Chin Woi and Tan, Wen Siang and Chong, Fui Chin and Ling, Tau Chuan and Tey, Beng Ti (2009) A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography. Journal of Microbiology and Biotechnology, 19 (4). pp. 416-423. ISSN 1017-7825; ESSN: 1738-8872 http://www.jmb.or.kr/journal/viewJournal.html?year=2009&vol=19&num=4&page=416 10.4014/jmb.0804.254 |
| spellingShingle | Ho, Chin Woi Tan, Wen Siang Chong, Fui Chin Ling, Tau Chuan Tey, Beng Ti A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography |
| title | A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography |
| title_full | A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography |
| title_fullStr | A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography |
| title_full_unstemmed | A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography |
| title_short | A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography |
| title_sort | preparative purification process for recombinant hepatitis b core antigen using online capture by expanded bed adsorption followed by size exclusion chromatography |
| url | http://psasir.upm.edu.my/id/eprint/12790/1/A%20preparative%20purification%20process%20for%20recombinant%20Hepatitis%20B%20core%20antigen%20using%20online%20capture%20by%20expanded%20bed%20adsorption%20followed%20by%20size.pdf |
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