A preparative purification process for recombinant Hepatitis B core antigen using online capture by expanded bed adsorption followed by size-exclusion chromatography
Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was...
Main Authors: | Ho, Chin Woi, Tan, Wen Siang, Chong, Fui Chin, Ling, Tau Chuan, Tey, Beng Ti |
---|---|
Format: | Article |
Language: | English |
Published: |
Korean Society for Microbiology and Biotechnology
2009
|
Online Access: | http://psasir.upm.edu.my/id/eprint/12790/1/A%20preparative%20purification%20process%20for%20recombinant%20Hepatitis%20B%20core%20antigen%20using%20online%20capture%20by%20expanded%20bed%20adsorption%20followed%20by%20size.pdf |
Similar Items
-
Purification of recombinant nucleocapsid protein of Newcastle disease virus from unclarified feedstock using expanded bed adsorption chromatography
by: Tan, Yan Peng, et al.
Published: (2006) -
Direct recovery of recombinant nucleocapsid protein of Nipah virus from unclarified Escherichia coli homogenate using hydrophobic interaction expanded bed adsorption chromatography
by: Chong, Fui Chin, et al.
Published: (2010) -
A preparative hydrophobic interaction chromatography for purification of recombinant nucleocapsid protein of Nipah virus from clarified Escherichia coli homogenate
by: Chong, Fui Chin, et al.
Published: (2010) -
Purification of histidine-tagged nucleocapsid protein of Nipah virus using immobilized metal affinity chromatography
by: Fui , Chin Chong, et al.
Published: (2009) -
Purification of His-tagged hepatitis B core antigen from unclarified bacterial homogenate using immobilized metal affinity-expanded bed adsorption chromatography
by: Yap, Wei Boon, et al.
Published: (2010)