A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus
A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification an...
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| Format: | Article |
| Language: | English |
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Society for General Microbiology
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/12859/1/A%20simplified%20multiplex%20PCR%20assay%20for%20fast%20and%20easy%20discrimination%20of%20globally%20distributed%20staphylococcal%20cassette%20chromosome%20mec%20types%20in%20meticillin.pdf |
| _version_ | 1825945124893360128 |
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| author | Ghaznavi-Rad, Ehsanollah Shamsudin, Mariana Nor Sekawi, Zamberi Belkum, Alex van Neela, Vasantha Kumari |
| author_facet | Ghaznavi-Rad, Ehsanollah Shamsudin, Mariana Nor Sekawi, Zamberi Belkum, Alex van Neela, Vasantha Kumari |
| author_sort | Ghaznavi-Rad, Ehsanollah |
| collection | UPM |
| description | A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCCmec types that we tested were correctly characterized by our PCR assay. SCCmec type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type. |
| first_indexed | 2024-03-06T07:26:38Z |
| format | Article |
| id | upm.eprints-12859 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:26:38Z |
| publishDate | 2010 |
| publisher | Society for General Microbiology |
| record_format | dspace |
| spelling | upm.eprints-128592015-11-27T03:00:50Z http://psasir.upm.edu.my/id/eprint/12859/ A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus Ghaznavi-Rad, Ehsanollah Shamsudin, Mariana Nor Sekawi, Zamberi Belkum, Alex van Neela, Vasantha Kumari A multiplex PCR assay was developed for the identification of major types and subtypes of staphylococcal cassette chromosome mec (SCCmec) in meticillin-resistant Staphylococcus aureus (MRSA) strains. The method uses a novel 9 valent multiplex PCR plus two primer pairs for S. aureus identification and detection of meticillin resistance. All 389 clinical MRSA isolates from Malaysia and 18 European isolates from the Harmony collection harbouring different SCCmec types that we tested were correctly characterized by our PCR assay. SCCmec type III and V were by far the most common types among both hospital- and community-acquired Malaysian MRSA isolates, with an apparent emergence of MRSA harbouring the IVh type. Society for General Microbiology 2010-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/12859/1/A%20simplified%20multiplex%20PCR%20assay%20for%20fast%20and%20easy%20discrimination%20of%20globally%20distributed%20staphylococcal%20cassette%20chromosome%20mec%20types%20in%20meticillin.pdf Ghaznavi-Rad, Ehsanollah and Shamsudin, Mariana Nor and Sekawi, Zamberi and Belkum, Alex van and Neela, Vasantha Kumari (2010) A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus. Journal of Medical Microbiology, 59 (10). pp. 1135-1139. ISSN 0022-2615; ESSN: 1473-5644 http://jmm.sgmjournals.org/content/59/10/1135.abstract 10.1099/jmm.0.021956-0. Epub 2010 Jul 8 |
| spellingShingle | Ghaznavi-Rad, Ehsanollah Shamsudin, Mariana Nor Sekawi, Zamberi Belkum, Alex van Neela, Vasantha Kumari A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus |
| title | A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus |
| title_full | A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus |
| title_fullStr | A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus |
| title_full_unstemmed | A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus |
| title_short | A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus |
| title_sort | simplified multiplex pcr assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin resistant staphylococcus aureus |
| url | http://psasir.upm.edu.my/id/eprint/12859/1/A%20simplified%20multiplex%20PCR%20assay%20for%20fast%20and%20easy%20discrimination%20of%20globally%20distributed%20staphylococcal%20cassette%20chromosome%20mec%20types%20in%20meticillin.pdf |
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